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PNIPAAm-PEG a block co-polymer of poly(N-isopropylacrylamide) and poly(ethylene glycol) for 3D Cell Culture and other applications

Mebiol®Gel PNIPAAm-PEG 3D Thermoreversible Hydrogel


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Background


Mebiol (R) Gel 
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Hydrogels are a diverse class of polymeric materials characterized by their network-like structure and high water content. Hydrogels of many kinds have found a wide variety of applications in medicine and life science research weighted towards, but not at all limited to three-dimensional cell culture, tissue engineering, and drug delivery. Properties highly favorable to cell culture and tissue engineering applications prompted the commercialization of Mebiol Gel, a copolymer of poly(N-isopropylacrylamide) and poly(ethylene glycol) (PNIPAAm-PEG) for research purposes in the early 2000's.

Mebiol Gel's defining feature, in contrast to other commercially available hydrogels, is its temperature reversible sol-gel transition. When cooled, Mebiol Gel is a sol (handles like a liquid) but becomes a rigid hydrogel at higher temperatures. In practice, this means extremely easy cell handling. Cultures are seeded into cooled Mebiol Gel and recovered conveniently by cooling the culture vessel and centrifugation. In the gel state, the highly lipophylic environment of the Mebiol Gel presents an efficient niche for cell proliferation, cell communication, gas and mass exchange, and protection of cells and tissue from shear forces.

           

                      Low Temperature (Sol)                                  High Temperature (Gel)

 

Features

¥@Easy handling
¥@Non-toxic, biocompatible
¥@100% synthetic, pathogen free
¥@High transparency for cell observation
¥  Proven performance.

Application

¥@Stem cell and pluripotent stem cell culture, expansion, and differentiation
¥@Spheroid culture
¥@Cell implantation
¥@Organ and Tissue Regeneration
¥@Drug Delivery
¥@Non-cell culture application

Freeze-drying Polymer is put in each flask as follows. 
  • MBG-PMW20-1001 (10mL) : 1g 
  • MBG-PMW20-5001 (50mL) : 5g

Application Examples

1) Culture of primary cancer cells in Mebiol Gel
Selective growth of only primary cancer cells from human cancerous tissue in Mebiol Gel (courtesy Dr. S. Kubota, Dept. of General Surgery, St. Marianna University School of Medicine). This technology enables the characterization of patient-derived primary cancer cells and therefore enabling the evaluation of primary cells for chemosensitivity, malignancy, metastasis activity and other parameters that might influence patient therapy.

Human colon cancerous tissue was cultured in Mebiol Gel for 10 days.Only primary cancer cells proliferate from the tissue in Mebiol Gel.

Fibroblasts growth in Mebiol Gel is suppresed whereas In collagen and many other 3D gel culture matrices, fibroblasts overgrow and prevent proliferation of primary cancer cells.

         

2) Stem Cell Culture

3D culture of undifferentiated mouse and Macaca ES cells cultured without LIF or feeder layer cells performed in collaboration with with Dr. K. Hishikawa, Dept. of Clinical Renal Regeneration, University of Tokyo.

Left: 2D on Feeder Cells
Right: 3D Culture in Mebiol Gel
          (Day 7)

 

The strong positive alkaline phosphatase staining of Macaca (primate) ES cells cultured in Mebiol Gel® suggests undifferentiation.

Left: 2D on Feeder Cells
Right: 3D Culture in Mebiol Gel
          (without LF, day 5)

 

3) Selective separate culture of somatic stem cell (mouse embryo skin origin)

Epithelial Stem Cells from Dermis by a Three-dimensional Culture System”
Journal of Cellular Biochemistry, 98 (1), 174-184 (2006)

4) Cartilage tissue reproduction by in vitro 3-D culture of cartilage cell
Chondrocytes Containing Growth Factors in a Novel Thermoreversible Gelation Polymer Scaffold” Tissue Engineering, 12 (5), 1237-1245 (2006)

5) Bone induction by in vitro 3-D culture of human mesenchymal cell stem cell (hMSC)
“Gene expression profile of human mesenchymal stem cells during osteogenesis in three-dimensional thermoreversible gelation polymer”
Biochem. Biophys. Res. Commun., 317, 1103-1107 (2004).

6) Production of hepatitis C viruses (HCV) by 3-D culture of human hepatocyte cell lineE
“Production of infectious hepatitis C virus particles in three-dimensional cultures of the cell line carrying the genome-length dicistronic viral RNA of genotype 1b“@
Virology, 351 (2), 381-392 (2006)

7) Passage control by local heating (on chip cell sorter system)
“On-Chip Cell Sorting System Using Laser-Induced Heating of a Thermoreversible Gelation Polymer to Control Flow”, Y. Shirasaki, J. Tanaka, H. Makazu, K. Tashiro, S. Shoji, S. Tsukita, T. Funatsu,
Anal. Chem., 78, 695-701 (2006)

Technical Notes

Product List

This product can not be sold to customers in India


Product Name Cat# Quantity Price

Mebiol(R) Gel DataSheet ,

MBG-PMW20-1001

1*10 ML ¥30,000
$400

Mebiol(R) Gel DataSheet ,

MBG-PMW20-1005

5*10 ML ¥100,000
$1334

Mebio(R) Gel DataSheet ,

MBG-PMW20-1020

20*10 ML contact us

Mebiol(R) Gel DataSheet ,

MBG-PMW20-5001

1*50 ML ¥60,000
$800

Mebiol(R) Gel DataSheet ,

MBG-PMW20-5005

5*50 ML ¥200,000
$2667

Mebiol(R) Gel DataSheet ,

MBG-PMW20-5020

20*50 ML contact us

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