useful to a general oxidative stress marker in the body.

New 8-OHdG Check ELISA KIT Trial Package


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8-OHdG top

This product is a subset of KOG-200SE New 8-OHdG Check ELISA,
with less test samples and lower price.

What is 8-OHdG?
Oxidative stress is known to play an important role in the development of various diseases and aging process. 8-hydroxy-2?f-deoxyguanosine (8-OHdG) is formed when DNA is oxidatively damaged by reactive oxygen species (ROS). 8-OHdG is one of the most sensitive biomarker for oxidative stress and can be detected in urine, serum, tissue DNA from human and animals. It should be noted that oxidative stress can be assessed non-invasively using urinary 8-OHdG.

Features of 8-OHdG Trial kit

1) High specificity high sensitivity:
Measuring range: 0.5 to 200 ng/mL. 50 micro L / test.
Cross reactivity was checked for 19 analogues.
dA, dC, dT, 2?f-dG, 8-OHdA, 2?f-dI, 2?f-dU, O6-methyl-dG, G, 7-methyl-G, 8-Br-G, 8-sulfhydryl-G, 4-SH-G, 8-OH-G, Gua, O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatinine, creatine (G: Guanosine, Gua: Guanine)

2) Easy operation and speedy:
There is no need for expensive equipment and sample pretreatment. Assays can be completed within 3.5 hours.
Sample volume required: 50 micro L (N=1)

3) Cost effective: 427US$, 345EURO/ 32 wells
Suitable for small-scale experiments. Contains 8 wells * 4 splits (total 32 wells).

Kit Contents

1. A 8-OHdG-coated microplate wells (32 wells).
2. Primary antibody (Anti 8-OHdG monoclonal antibody N45.1).
3. Primary antibody dissolving solution.
4. Secondary antibody (HRP-conjugated anti-mouse antibody).
5. Secondary antibody dissolving solution.
6. Chromatic solution (TMBZ).
7. Substrate solution.
8. Washing solution.
9. Reaction terminating solution.
10. Standard 8-OHdG solution(0.5, 2, 8, 20, 80, 200 ng/ml).
11. Plate seal

Required but not provided

50fÊl pipettor and tips.
8-channel micropipettor and tips.
Trays for 8-channel pipettor.
Incubator (37 degree C).
Distilled water.
96 wells Microplate reader (450nm).

<Reference>
1. S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi, and T.Osawa:
Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model.
Lab. Invest. 76(3), p365-374 (1997)
2. M.Erhola, S.Toyokuni, K.Okada, T.Tanaka, H.Hiai, H.Ochi, K.Uchida, T.Osawa, M.M.Nieminen, H.Alho, and P.K-Lehtinen:
Biomarker evidence of DNA oxidation in lung cancer patients: association of urinary 8-hydroxy-2?Edeoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment.
FEBS Lett. 409, p287-291 (1997)
3. J.Leinonen, T.Lehtimaki, S.Toyokuni, K.Okada, T.Tanaka, H.Hiai, H.Ochi, P.Laippala, V.Rantalaiho, O.Wirta, A.Pasternack, and H.Alho:
New biomarker evidence of oxidative DNA damage in patients with non-insulin-dependent diabetes mellitus.
FEBS Lett. 417, p150-152 (1997)
4. W. Y. Fan, K. Ogusu, K. Kouda, H. Nakamura, T. Satoh, H. Ochi and H. Takeuchi:
Reduced oxidative DNA damage by vegetable juice intake: A controlled trial.
J. Physiol. Anthropol. 19(6), p287-289 (2000)
5. S.Saito, H.Yamauchi, Y.Hasui, J.Kurashige, H.Ochi and K.Yoshida:
Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OHdG) by using ELISA.
Res. Commun. Mol. Pathol.Pharmacol. 107, p39-44

Product List

Product Name Cat# Quantity Price

8-OHdG Check ELISA Trial Package (32wells) DataSheet ,

NNS-KOG-200TE-EX

32 WELL N/A

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