Reagents for oxidative stress research

Anti 4-HNE Monoclonal Antibody


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Background


4-Hydroxy-2-Nonenal
Immunohistochemcal detection of 4-HNE in colorectal carcinoma cells. Adenocarcinoma (arrows) and non tumorous epithelial cells (star).
(S. Kondo, et al.:Free Radical Biology & Medicine 27, p401-410, 1999)

Source Mouse
Immunogen 4-HNE-modified Keyhole Limpet Hemocyanin
Subclass IgG1(kappa)
Clone HNEJ-2
Form Lyophilized Powder
Specificity This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.
Applications Immunohistochemistry and Western blotting.
Storage Less than -20°C for 5 years
List of references
1) S.Toyokuni, N.Miyake, H.Hiai, M.Hagiwara, S.Kawakishi, T.Osawa and K.Uchida:
The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct.
FEBS Lett. 359, p189-191 (1995)
2) T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni:
Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion.
Lab. Invest. 77(2), p145-155 (1997)
3) Y.Hattori, C.Nishigori, T.Tanaka, K.Uchida, O.Nikaido, T.Osawa, H.Hiai, S.Imamura, and S.Toyokuni:
J. Invest. Dermatol. 107, p733-737 (1997)
4)

S.Toyokuni:
Reactive oxygen species-induced molecular damage and its application in pathology.

Pathol. Internat. 49, p91-102 (1999)

5) S.Kondo, S.Toyokuni, Y.Iwasa, T.Tanaka, H.Onodera, H.Hiai and M.Imamura:
Persistent oxidative stress in human colorectal carcinoma, but not in adenoma.
Free Rad. Biol. Med. 27, p401-410 (1999)
6)

T.D. Oberley, S.Toyokuni, and L.I.Szweda:
Localization of hydroxynonenal protein adducts in normal human kidney and selected human kidney cancers.
Free Rad. Biol. Med. 27, p695-703 (1999)

Product List

Product Name Cat# Quantity Price

Anti 4-HNE (Mouse)  DataSheet

NNS-MHN-100P-EX

100 UG ¥89,600
$1195

Anti 4-HNE (Mouse)  DataSheet

NNS-MHN-020P-EX

20 UG ¥23,100
$308

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