Novel protein oxdation marker

Anti dityrosine (DT) monoclonal antybody


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Background

Tyrosine is one of the major targets of protein oxidation, and until today various tyrosine derivatives such as nitrotyrosine, dityrosine and halogenated tyrosine depending on the type of free radicals. DT is a tyrosine dimer derived from tyrosyl radicals which is formed by reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. DT have been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains. Dityrosine is one of the specific biomarkers for protein oxidation

Anti dityrosine monoclonal antibody clone 1C3 has been established by Dr. Toshihiko Osawa (Nagoya Univ.) and Dr. Yoji Kato (Univ. Hyogo)1). They have prepared this antibody using a dimer of 3-(p-hydroxyphenyl) propionic acid as a hapten, and screened using dityrosine-conjugated BSA. This antibody is highly specific for dityrosine, and can react with both free dityrosine and dityrosine containing protein or peptides. Suitable for immunohistochemistry, western blotting and ELISA.


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Application

Immunohistochemistry, western blotting and ELISA

< Specificity >
Specific for free dityrosine, 3-(p-hydroxyphenyl) propionic acid dimer, dityrosine-BSA conjugate, and dityrosine in protein or peptides. Cross reactivity have been tested for following analogues: Nitrotyrosine, chlorotyrosine, aminotyrosine, phosphotyrosine, tyrosine, 3-(p-hydroxyphenyl) propionic acid, phenylalanine, 3,4-dihydroxyphenylacetic acid (DOPA)

 

< Applicable species >
This antibody can be applied to tissue samples from human, rabbit, rat and other animals, because lipid oxidation is species independent.
But if you are planning to apply to mouse tissue, blocking of internal mouse IgG will be needed before staining. For example, Vector M.O.M. Immunodetection kit (code.PK2200) may be useful for blocking of mouse IgG.

 

< Immunohistochemistry >

DT staining of mouse skin (Dr. Sugiyama, Tottori Univ.)

 

< Reference >
1) Kato Y, Wu X, Naito M, Nomura H, Kitamoto N, Osawa T.
Immunochemical detection of protein dityrosine in atherosclerotic lesion of apo-E-deficient mice using a novel monoclonal antibody.
Biochem Biophys Res Commun. 275(1), p11-15 (2000).

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Product List

Product Name Cat# Quantity Price

Anti Dityrosine (DT), Mouse (Mouse) Unlabeled DataSheet

NNS-MDT-020P-EX

20 UG ¥30,800
$411

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