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a general oxidative stress marker in the body

Anti Dibromo-Tyrosine (DiBrY) monoclonal antibody


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Background

Tyrosine is one of the main targets of protein oxidation, and it have been reported form nitrotyrosine, dityrosine and halogenated tyrosines depending on the type of free radicals. For example, hypochlorous acid (HOCl) and hypobromous acid (HOBr) are derived from neutrophil myeloperoxidase (MPO) and eosinophil peroxidase (EPO), and known to form dihalogenated tyrosines such as DiBrY, DiClY and DiIY. It is expected that DiBrY may be a novel biomarker for tissue damage which is related to inflammatory and allergic disorders.

<dibromotyrosine>
Anti dityrosine monoclonal antibody clone 1C3 has been established by Dr. Toshihiko Osawa (Nagoya Univ.) and Dr. Yoji Kato (Univ. Hyogo)1). They have prepared this antibody using a dimer of 3-(p-hydroxyphenyl) propionic acid as a hapten, and screened using dityrosine-conjugated BSA. This antibody is highly specific for dityrosine, and can react with both free dityrosine and dityrosine containing protein or peptides. Suitable for immunohistochemistry, western blotting and ELISA.

Monoclonal antibody clone 3A5 have been established by Dr. Toshihiko Osawa (Nagoya Univ.) and Dr. Yoji Kato (Univ. Hyogo). This antibody is specific for 3,5-Dibrominated tyrosine and other di-halogenated tyrosine at 3- and 5- position. Suitable for immunohistochemical analysys of oxidative stress.

Application

Immunohistochemistry, western blotting and ELISA

Immunoshistochemical protocolPDF

<Specificity>
This antibody can react with dibromotyrosine (DiBrY), N-acetylated dichloro tyrosine (NAcDiClY), diiodo tyrosine (DiIY), N-bromosuccinimide-treated BSA, HOCl-treated BSA and dibromo-tyrosone conjugated BSA (halogene-modified tyrosine at both 3- and 5-position).
Cross reactivity to following analogues have been tested:
dityrosine-conjugated BSA, peroxynitrite-treated BSA, tyrosine (Y), chrolotyrosine(ClY), 3-3’-dityrosine (DiY), 3-aminotyrosine (NH2Y), 3-nitrotyrosine (NO2Y), phenylalanine (Phe), o-phosphotyrosine, 3,4-dihydroxyphenylacetic acid (DOPA) and 2-hydroxy-4-phenylacetic acid. Weakly react with Bromotyrosine (BrY).

<Applicable species>
This antibody can be applied to tissue samples from human, rabbit, rat and other animals, because lipid oxidation is species independent. But if you are planning to apply to mouse tissue, blocking of internal mouse IgG will be needed before staining. For example, Vector M.O.M. Immunodetection kit (code.PK2200) may be useful for blocking of mouse IgG.

[ Oxidative modification of tyrosine ]
Tyrosine is one of the main targets of protein oxidation, and it have been reported form nitrotyrosine, dityrosine and halogenated tyrosines depending on the type of free radicals. For example, hypochlorous acid (HOCl) and hypobromous acid (HOBr) are derived from neutrophil myeloperoxidase (MPO) and eosinophil peroxidase (EPO), and known to form dihalogenated tyrosines such as DiBrY, DiClY and DiIY. It is expected that DiBrY may be a novel biomarker for tissue damage which is related to inflammatory and allergic disorders.

[ Halogenated tyrosine related diseases ]
Cystic fibrosis: VD Vliet, et.al.: Myeloperoxidase and protein oxidation in cystic fibrosis. Am J Physiol Lung Cell Mol Physiol. 279, L537-L546 (2000)
Atherosclerotic intima: Hazen SL: 3-chlorotyrosine, a specific marker of myeloperoxidase ? catalyzed oxidation, is markedly elevated in low density lipoprotein isolated from human atherosclerotic intima.J Clin Invest 99, p2075-2081 (1997)
Sepsis: Gaut JP, et.al.: Neutrophils employ the myeloperoxidase system to generate antimicrobial brominating and chlorinating oxidants during sepsis. PNAS 98, p11961-11966 (2001)
Asthma: Wu W, et. al.: Eosinophils generate brominating oxidants in allergen - induced asthma. J Clin Invest 105, p1455-1463 (2000)

<Reference>
1) Y Kato, Y Kawai, H Morinaga, H Kondo, N Dozaki, N Kitamoto and T Osawa. Immunogenicity of a brominated protein and successive establishment of a monoclonal antibody to dihalogenated tyrosine. Free Rad Biol Med, 38, p24-31 (2005)
2)Wu W, Chen Y, d'Avignon A, Hazen SL. 3-Bromotyrosine and 3,5-dibromotyrosine are major products of protein oxidation by eosinophil peroxidase: potential markers for eosinophil-dependent tissue injury in vivo. Biochemistry.38(12):3538-48 (1999)

Product List

Product Name Cat# Quantity Price

Anti Dibromo-Tyrosine (DiBrY) (Mouse) Unlabeled DataSheet

NNS-MBY-020P-EX

20 UG ¥30,800
$411

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