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Blocking Reagents for Immunological Measurements

Blocking Reagent-NB2025,NB3025 -Synthetic polymer type blocking reagent-


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Background

This reagents for assay consist mainly of synthetic polymers and contain no materials derived from animals, so there is no danger of infection (such as Creutzfeldt-Jacob disease) from animals. Since synthetic polymers are the main components, all lots are quite uniform, and they can be boiled and autoclaved to sterilize and deactivate DNase. This reagents for assey are also highly stable against freezing, agitation, etc.

Blocking and Stabilizer reagent flyerPDF

Feature and Advantages

¥ Blocking Reagent for Immunoassay
¥ Do not contain any bio-derived materials
¥ Excellent effect to non-specific binding

Usage

¥ Use it like usual blocking reagents
¥ Use from 1 to 25-fold dilution
---recommend 5-fold dilution by saline---
¥ Recommend dry-treatment after blocking#Precautions for use The product contain 0.09% sodium azide as a preservatives. Be careful of intake by mistake or waste.

Characteristics

¥ There are two products, NB2025 and NB3025 synthetic polymers, which are different in molecular structure.
¥ Highly effective for restraining nonspecific absorption [Figure 1]
¥ Highly effective for stabilizing physiologically active substances that have been immobilized on carriers [Figure 2]
¥ NB2025 is excellent for stabilization whereas NB3025is optimum for restraining nonspecific absorption. However, evaluation before each experiment is recommended.
¥ Liquid form enabling immediate use after dilution
¥ Suitable as sample dilution and cleaning solution
¥ Highly stable to Tween 20. [Figure 3]

Blocking

Figure 1 The amount of non-specific adsorbed POD-conjugate
Immunoplate well (Maxisorp F96, Nunc) is treated with a 5-fold diluted blocking reagent (at room temp., 15-60 min.), POD- conjugate solution was added and incubated at 37°C for 120 min. After washing with PBS containing 0.05% tween20 (PBST), added color reagent for POD (H2O2-TMBZ system) and evaluated the amount of POD which remained in the well. No significant difference between Casein and yNB2025, NB3025 z.
Stabilization of Immobilized-Antibody

Figure 2 Remaining activity of immobilized-antibody that was stored at 50°C
Immunoplate well (Maxisorp F96, Nunc) is immobilized with anti-mouse-IgG-antibody. After blocking with 5-fold diluted blocking reagent (at room temp., 120min.), the plate which has de-air-packed in aluminum bag, was stored in 50°C. The activity of immobilized-antibody is evaluated with sandwich-ELISA system.
Tolerance against detergent

Figure 3 The amount of non-specific adsorbed POD-conjugate
After blocking (see above), POD-conjugate diluted with PBST was added and incubated at 37°C for 120 min. After washing with PBST, added color reagent for POD (H2O2-TMBZ system) and evaluated the amount of POD which remained in the well.
Product List

Product Name Cat# Quantity Price

Blocking Reagent NB2025 DataSheet ,

NOF-51005011

250 ML N/A

Blocking Reagent NB3025 DataSheet ,

NOF-51005012

250 ML N/A

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To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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