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Food Allergen Screening Test Kit

FASTKIT ELISA "PEANUT," "EGG," "WHEAT,""BUCK WHEAT," "MILK," and "SOYBEAN"


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Background

Amendments to Food Sanitation Law in Japan have rendered it mandatory to label 5 specific raw food materials, (Eggs, Milk, Wheat, Buckwheat and Peanuts), identified to have a high possibility of triggering food allergies when foods contain more than a predetermined amount of µg/mL or µg/g of the specified raw ingredients, as of April 2002. In addition to clearly labeling the fore mentioned food products, screening of such products is becoming increasingly important in order to confirm the amount of proteins into contained in the raw ingredient or confirm the existence by a result of contamination due to the mixing of these raw materials with other foodstuffs and contamination on production lines. FASTKIT ELISA Version II Peanut is a test kit for detecting peanut proteins in foodstuffs by using enzyme-linked immunosorbent assay (ELISA). This kit is an enhanced version of FASTKIT ELISA PEANUT with a greater efficiency in terms of peanut protein extraction. The kit complies with the notification Monitoring Methods for Foods With Allergic Substances (#1106001, #1011002) issued by the Pharmaceutical and Food Safety Bureau of the Ministry of Health, Labor, and Welfare.* [Characteristics]

1. The FASTKIT ELISA PEANUT is able to accurately measure the amount of peanut proteins in foods.
2. The FASTKIT ELISA PEANUT can simultaneously identify the presence of multiple peanut proteins.
3. The FASTKIT ELISA PEANUT is widely applicable to samples ranging from raw ingredients to processed foods.

Product Features

¥ The present monitoring kit can measure the peanut proteins in foods.
¥ The kit can measure the standard peanut protein at concentrations ranging from 0.78 to 50 ng/mL.*

Components of the Kit

A. Antibody-immobilized microtiter plate (with a cover) A 96-well microtiter plate
(8 wells x 12 lanes) x 1
B. Standard solution (50 ng/mL);manufactured by Nippon Gean Co., Ltd. 1.8 mL x 1
C. Dilution buffer 100 mL x 1
D. Biotin-conjugated antibody 150 µL x 1
E. Streptavidin-conjugated enzyme (peroxidase) 150 µL x 1
F. Chromogenic substrate (TMB) 12 mL x 1
G. Solution to stop reaction (0.5N H2SO4) 12 mL x 1
H. Condensed wash solution (10-fold condensate) 100 mL x 1
I. Condensed extraction buffer 1. (20-fold condensate) 50 mL x 1
J. Condensed extraction buffer 2. (20-fold condensate) 50 mL x 1
K. Condensed extraction buffer 3. (20-fold condensate) 50 mL x 1
L. Instruction leaflet 1 copy

Screening Principles

1. Various antibodies that recognize peanut proteins solidify in the wells of the microtiter plate.
2. Various peanut proteins (e1, e2…) from a sample are trapped by the solidifying antibodies, and peanut protein-antibody complexes are produced.
3. Biotin-conjugated antibodies are bound to these complexes to form sandwich complexes.
4. Streptavidin-conjugated enzymes are bound to the complexes through biotin–streptavidin reactions, and then the enzyme substrate (TMB) is added to develop the color.

Product List

Product Name Cat# Quantity Price

FASTKIT ELISA Ver. II EGG DataSheet ,

NPH-999100420EX

1 KIT see also
# NPH-999100430EX
Size 1

FASTKIT ELISA Ver. II WHEAT, Wheat DataSheet

NPH-999100421EX

1 KIT N/A

FASTKIT ELISA Ver. II PEANUT, Peanut DataSheet

NPH-999100422EX

1 KIT N/A

FASTKIT ELISA Ver. II BUCKWHEAT DataSheet ,

NPH-999100423EX

1 KIT N/A

FASTKIT ELISA Ver. II MILK DataSheet ,

NPH-999100424EX

1 KIT N/A

FAST ELISA Ver. II Soybean, Soybean DataSheet

NPH-999100425EX

1 KIT N/A

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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