To detect Campylobacter in foods

NH IMMUNOCHROMATO CAMPYLOBACTER


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Background

There are known to be 17 species of Campylobacter as of 2005. Among them, Campylobacter jejuni and Campylobacter coli are designated as food poisoning bacteria 1).
Campylobacter food poisoning is extremely important for food hygiene, because in Japan, it has recently been one of the leading causes of food poisoning and ranked as the top cause in 2007. Contamination with Campylobacter has been frequently observed in chicken, so it is necessary to not only prevent secondary contamination from chicken, but also to take preventive measures at chicken farms and chicken processing factories 2).
This product is a kit for detecting Campylobacter in foods by immunochromatography. Tests can be conducted rapidly and simply by means of the kit.

Application

1) Detection of Campylobacter species (C. jejuni and C. coli) in foods

Features

#  The simple one-step operation of the kit.
#  The test gives rapid results.
#  There is no need for special test equipment.

1)  Sensitivity test
When the test is performed according to the “Preparation of the Sample Solution” and “Operating Procedures for Testing” in the manual, the test kit will show a positive result when C. jejuni is present at 1 × 105 CFU/mL or higher.
.

2)  Reproducibility test
When positive and negative test solutions of C. jejuni are simultaneously tested three times each, all positive test solutions exhibit positive results and all negative test solutions show negative results.

3)  Minimum detection sensitivity
The minimum detection sensitivity of the kit was confirmed to be 1 × 104 to 106 CFU/mL in the test with six standard strains of C. jejuni and 1 × 105 to 106 CFU/mL in the test with two standard strains of C. coli.
 Note 1:    The minimum detection sensitivity of this kit could vary depending on the effects of the components of the test solution.

4)  Cross-reactivity
(1) Cross-reactivity with the following bacterial strains has not been observed.

StrainsID of standard strainsResults
Campylobacter fetus subsp. fetus JCM2527 -
Campylobacter fetus subsp. venerealis JCM2528 -
Citrobacter freundii ATCC8090 -
Enterobacter aerogenes ATCC13048 -
Enterobacter cloacae ATCC13047 -
Enterobacter sakazakii ATCC51329 -
Escherichia coli O157 ATCC43888 -
Escherichia coli O26 RIMD05091876 -
Escherichia coli O1 ATCC11775 -
Escherichia hermannii JCM1473 -
Klebsiella pneumoniae ATCC4352 -
Klebsiella oxytoca ATCC8724 -
Pseudomonas aeruginosa ATCC9027 -
Proteus vulgaris ATCC6380 -
Salmonella Enteritidis ATCC13076 -
Salmonella Typhimurium ATCC13311 -
Serratia liquefaciens ATCC27592 -
Serratia marcescens ATCC8100 -
Serratia odorifera ATCC33077 -

(2) The test kit was found to be cross-reactive to Campylobacter lari and Campylobacter upsaliensis because they have the same antigen as C. jejuni and C. coli.

Illustration of Test plate and the Principle of assay

1) Part names

a. Sample dropping part (Take care not to touch it with your hands)
b. Reagent containing part
c. Spread area (Take care not to touch it with your hands or damage it)
d. Absorption pad
e. Measurement item entering area
f.  Test line appearance position (about 30 mm from the sample dropping part)
g.  Control line appearance position (about 38 mm from the sample dropping part)

2) Detection mechanism
When a sample solution is dropped at the sample dropping part of the test plate, the gold colloid labeled anti-Salmonella antibody (2) is dissolved to form complexes with Salmonella (1) in the sample solution.  The complexes migrate across the spread area by capillarity and are then trapped by the anti-Salmonella antibody (3) fixed at the test line appearance position to develop a reddish purple line by the gold colloid.  Thus, whether the sample solution contains Salmonella or not can be determined by visually inspecting the test line appearance position.
Irrespective of the presence/absence of Salmonella in the sample solution, the excess gold colloid labeled antibody further migrates across the spread area and is then trapped by the anti-rabbit immunoglobulin goat antibody (4) fixed at the control line appearance position to develop a reddish purple line.  It can be confirmed that the sample solution has migrated across the spread area by the development of the line.

Product List

Product Name Cat# Quantity Price

NH Immunochromato Campylobacter DataSheet ,

NPH-999600000

1 KIT ¥14,000
$187

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To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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