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Pin-point Protein Labeling An amber stop codon (UAG) is used for pin-point fluorescence or biotin labeling.

CloverDirect™ tRNA Reagents for Site-Directed Protein Functionalization_Details


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Background

CloverDirect™ tRNA Reagents for Site-Directed Protein Functionalization allow the incorporation of unnatural amino acids at defined positions of proteins using in vitro translation. Unnatural amino acids containing fluorescent groups, biotin, PEG, photo-crosslink, etc are available. Proteins with unnatural amino acids will be obtained within a few hours just by adding CloverDirect™ reagents and DNA template having an amber stop codon (UAG) or a four-base codon (CGGG) to an in vitro translation system. CloverDirect™ covers the following four applications. In addition, we provide custom services for the expression of proteins with unnatural amino acids (contact us).

Site-Directed Fluorescence Labeling

it is not easy to incorporate fluorescent groups into proteins in a site-direct and quantitative fashion by chemical modification. CloverDirect™ tRNA Reagents for Site-Directed Fluorescence Labeling allow the incorporation of fluorescent unnatural amino acids into proteins in a site-direct and quantitative fashion. Various fluorescent dyes are available including those for 488 nm, 543 nm and 633 nm excitation.

Site-Directed Biotin Labeling

CloverDirect™ tRNA Reagents for Site-Directed Biotin Labeling allow the incorporation of biotinylated unnatural amino acids into proteins in a site-direct and quantitative fashion. Labeling proteins are available for the oriented immobilization onto avidin-coated plates and beads. The biotinylated amino acids have one or two aminohexyl liners between amino acid and biotin.

Site-Directed Post-Translational Modification

It is not easy to prepare post-translationally modified proteins (phosphorylation, methylation, etc.). CloverDirect™ tRNA Reagents for Site-Directed Post-Translational Modification allow the incorporation of modified amino acids into proteins to obtain post-translationally modified proteins in a site-direct and quantitative fashion.

Site-Directed Unnatural Mutagenesis

By incorporation of unnatural amino acids containing functional groups, novel functional proteins can be designed and synthesized. CloverDirect™ tRNA Reagents for Site-Directed Unnatural Mutagenesis allow the incorporation of unnatural amino acids with PEG, photo-crosslinking, photo-isomerizable groups, etc.

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