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Antibodies for Aging Research

Anti SMP30 [Regucalcin,Gluconolactonase(GNL)] polyclonal antibody


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Background

SMP30 (Senescence Marker Protein-30) is a 34-kDa protein whose tissue levels in the liver, kidney, and lung decrease with aging (1, 2). SMP30 expressed in multiple tissues including the liver, kidney, brain, lung, adrenal gland, stomach, ovary, uterus, testis and epidermis. Recently, SMP30 was identified as the lactone-hydrolyzing enzyme gluconolactonases (GNL) of animal species (3). GNL is a key enzyme which involve in vitamin C (L-ascorbic acid) biosynthesis. SMP30 knockout mice displayed symptoms of scurvy when fed a vitamin C-deficient diet. Moreover, SMP30 protects mice lungs from oxidative stress associated with aging and smoking (4). Thus SMP30 is a key age-associated protein for aging, vitamin C and lung disease research.
š SMP30, Gluconolactonase and Regucalcin are all identical protein.

Immunohistochemical staining


Mouse liver stained with SMP30EGNL antibody at 1:300 dilution and developed by 3,3’-diaminobenzidine. Nucleus and cytoplasm of wild ype (SMP30+/+) mice stained, but not stained in liver from SMP30 knockout (SMP30-/-) mice.

Western Blot Analysis

Each lanes: Mouse Liver Extract
• Wild type Mouse : SMP30Y/+ and SMP30+/+
• SMP30 Knockout Mouse : SMP30Y/- and SMP30-/-
• Heterozygous Mouse : SMP30+/-
SMP30EGNL antibody at 1:1,000 dilution used.

Immunofluorescence staining


Primary cultured mouse hepatocytes stained with SMP30EGNL antibody at 1:200 dilution. Nucleus and cytoplasm stained in green.

 

<Reference>
1. Ishigami, A. et al., Senescence marker protein-30 knockout mouse liver are highly susceptible to TNF-alpha- and Fas-mediated apoptosis. Am. J. Pathol. 161 1273-1281 (2002)
2. Ishigami, A. et al., Nuclear localization of senescence marker protein-30 (SMP30) in cultured mouse hepatocytes and its homology to RNA polymerase. Biosci. Biotechnol. Biochem. 67 158-160 (2003)
3. Kondo, Y. et al., Senescence Marker Protein 30 Functions as Gluconolactonase in L-Ascorbic Acid Biosynthesis and Its Knockout Mice Are Prone to Scurvy. Proc. Nat. Acad. Sci. USA 103 5723-5728 (2006)
4. Sato, T. et al., Senescence Marker Protein-30 Protects Mice Lungs from Oxidative Stress, Aging and Smoking. Am. J. Respir. Crit. Care Med. 174 530-537 (2006)

Product List

Anti SMP30 [Regucalcin, Gluconolactonase (GNL)


Product Name Cat# Quantity Price

Anti SMP30 [Regucalcin, Gluconolactonase (GNL)], Rat (Rabbit) Unlabeled DataSheet

SML-ROI001-EX

0.1 ML ¥30,000
$400

Anti PAD2(Peptidylarginine Deiminase 2) polyclonal antibody


Product Name Cat# Quantity Price

Anti PAD2, Human (Rabbit) Unlabeled DataSheet

SML-ROI002-EX

0.1 ML ¥30,000
$400

Anti GFAP(Glial Fibrillary Acidic Protein) polyclonal antibody


Product Name Cat# Quantity Price

Anti GFAP, Human (Rabbit) Unlabeled DataSheet

SML-ROI003-EX

0.2 ML ¥30,000
$400

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