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very convenient tool for evaluating sugar chain - protein interaction

Gold Nano Particles for Bioseparation SGNP (Sugar chain immobilized Gold Nano Particle)


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Background

SGNP (Sugar chain - immobilized Gold Nano-Particle) is a gold nano particle immobilized with structurally defined sugar chains, showing purple-red color (Abs. max = ca. 530 nm).@In the present product (Heparin-GNP), heparin, a sulfated polysaccharide, is immobilized.The diameter of particle is ca.15 to 30 nm.

Application

[Application#1]
Concentration and Purification of Heparin Binding Sample (Protein, Virus, etc.) from a Crude Extract

Heparin binding sample can be easily concentrated/captured by mixing Heparin-GNP with a crude extract, followed by the centrifugation. The concentrated/captured heparin binding sample with Heparin-GNP can be analyzed by SDS-PAGE, Western-blotting, real-time PCR, etc.

Example for Identification-1: SDS-PAGE and Western-blotting
To the precipitate obtained using the protocol, add 10-30 μl of sample preparation buffer*, and boil for 10 min. The sample is directly analyzed by SDS-PAGE and Western-blot.

Example for Identification-2: Real-time PCR
To the precipitate obtained in the protocol, add 10 μl of H20, and boil for 10 min. The boiled samples are ready to use for Real-time PCR

[Application #2]
Probe for Dot-Blot Assay
The samples ability to bind to heparin can be visualized by soaking the nitrocellulose membrane spotted with sample in a solution of Heparin-GNP.

Product List

Product Name Cat# Quantity Price

Heparin-GNP ,

SXB-GC-HEP-500-EX

1 VIAL N/A

SGNPs : *One vial contains each different SGNP showing Abs530nm = 3.0[/cm] when the whole SGNP is dissolved in 1 mL of buffer.


Product Name Cat# Quantity Price

(α)Glucosyl GNP ,

SXB-G-00M-250-EX

1 VIAL ¥7,000
$94

(β)Glucosyl GNP ,

SXB-G-00C-250-EX

1 VIAL ¥7,000
$94

(α)Galactosyl GNP ,

SXB-G-00E-250-EX

1 VIAL ¥7,000
$94

(β)Galactosyl GNP ,

SXB-G-00L-250-EX

1 VIAL ¥7,000
$94

(β)GlcNAc GNP ,

SXB-G-BGN-250-EX

1 VIAL ¥18,000
$240

(α)GalNAc GNP ,

SXB-G-AAN-250-EX

1 VIAL ¥18,000
$240

(β)GalNAc GNP ,

SXB-G-BAN-250-EX

1 VIAL ¥18,000
$240

(α)Fucosyl GNP ,

SXB-G-0AF-250-EX

1 VIAL ¥18,000
$240

(β)Fucosyl GNP ,

SXB-G-0BF-250-EX

1 VIAL ¥18,000
$240

(α)Mannosyl GNP ,

SXB-G-AMA-250-EX

1 VIAL ¥9,000
$120

3SialylGalactosyl GNP ,

SXB-G-03S-250-EX

1 VIAL ¥18,000
$240

6SialylGalactosyl GNP ,

SXB-G-06S-250-EX

1 VIAL ¥18,000
$240

SGNP set : *Each vial in a set contains SGNP showing Abs530nm = 3.0m/cmn, when the whole SGNP is dissolved in 0.25 mL of buffer


Product Name Cat# Quantity Price

SGNPs#1 [Sugar immoblized Gold Nano particles #1] DataSheet ,

SXB-G-AB1-250-EX

1 SET ¥140,000
$1867

Contains following 11 kinds of SGNPs in a set. Alpha-Glucosyl-GNP; Beta-Glucosyl-GNP; Alpha-Galactosyl-GNP; Beta-Galactosyl–GNP; Alpha-GlcNAc-GNP; Beta-GlcNAc-GNP; Alpha-GalNAc-GNP; Beta-GalNAc-GNP; Alpha-Fucosyl-GNP, Beta-Fucosyl-GNP, Alpha-Mannosyl-GNP


Product Name Cat# Quantity Price

SGNPs#2 [Sugar immoblized Gold Nano particles #2] DataSheet ,

SXB-G-AB2-250-EX

1 SET ¥170,000
$2267

Contains following 13 kinds of SGNPs in a set. Alpha-Glucosyl-GNP; Beta-Glucosyl-GNP; Alpha-Galactosyl-GNP; Beta-Galactosyl–GNP, Alpha-GlcNAc-GNP; Beta-GlcNAc-GNP; Alpha-GalNAc-GNP, Beta-GalNAc-GNP, Alpha-Fucosyl-GNP; Beta-Fucosyl-GNP; Alpha-Mannosyl-GNP; 3SialylGalactosyl –GNP; 6SialylGalactosyl –GNP

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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