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Enhancing antigen-antibody reaction in Western blotting?dot blotting and ELISA

Can Get Signal® Immunoreaction Enhancer Solution


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Feature and Advantages

1: High signal and low background

  • This solution greatly enhances antigen-antibody reactions, yielding signals several ten times higher than obtained with conventional methods.
  • Two kinds of solution is optimized for primary and secondary antibodies, which can suppress the background and increase the S/N ratio.

2: Wide versatility

  • It is applicable to various assay system involving antigen-antibody reactions such as Western blotting, dot blotting and ELISA.
  • It is compatible with all of the detection system involving chemical coloration, chemiluminescence and fluorescence. The composition has no affect on the activity of labeled antibodies such as peroxidase and alkaline phosphatase.

3: Ready to use

  • It is supplied at a ready-for-use concentration.
  • It can be directly used as an antibody diluent instead of the PBS or TBS diluents used with conventional methods.

 

Example

 

Detection of phosphorylated protein by Western blotting

Fig 1 shows the result for detecting phosphorylated Akt. Detection of phosphorylated Akt increased as the insulin concentration increased. There was a clear signal enhancement and background suppression in the assay using Can Get Signal compared with the conventional method using TBS-T. This result suggest the use of Can Get Signal yield higher signals and lower backgrounds in Western blotting with antibodies against phosphprylated protein compared with conventional assay methods using TBS-T as a diluent.

(* This data was provided by Dr. Toshihiko Yanagita of Pharmacology, Faculty of Medicine, University of Miyazaki)

 


Fig 1 Detection of phosphorylated Aktt with anti-phpspho-Akt antibody

Sample: Cultured bovine adrenomedullary cells

1. Control (H2O)
2. Insulin (1 nM, stimulated for 5min.)
3. Insulin (10 nM, stimulated for 5min.)
4. Insulin (100 nM, stimulated for 5min.)

Product List

Product Name Cat# Quantity Price

Can Get Signal Immunoreaction Enhancer Solution DataSheet ,

TYB-NKB-101

2*250 ML ¥40,100
$535

Can Get Signal Immunoreaction Enhancer Solution DataSheet ,

TYB-NKB-101T

2*50 ML ¥13,400
$179

Can Get Signal for primary antibody DataSheet ,

TYB-NKB-201

250 ML ¥22,700
$303

Can Get Signal for secondary antibody DataSheet ,

TYB-NKB-301

250 ML ¥22,700
$303

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TYB-NKB-401

2*5 ML ¥16,100
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Can Get Signal immunostain Solution A DataSheet ,

TYB-NKB-501

1*20 ML ¥40,100
$535

Can Get Signal immunostain Solution A DataSheet ,

TYB-NKB-502

4*20 ML N/A

Can Get Signal immunostain Solution B DataSheet ,

TYB-NKB-601

1*20 ML ¥40,100
$535

Can Get Signal immunostain Solution B DataSheet ,

TYB-NKB-602

4*20 ML N/A

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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