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GLP-1, gucagon-like peptide,insulin secretagogue,GLP1?

Glucagon Like Peptide-1 EIA Kit and Related products


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Background

GLP-1 is a peptide hormone from the intestinal mucosa, which is produced from its precursor, proglucagon by post transnational processing. The mammalian proglucagon is synthesized in the neuroendocrine L-cell of the intestine and the alpha-cells of the pancreas. It contains within its structure the sequences of glucagon and two glucagon-like peptides (GLP-1 and GLP-2) in tandem flanked at their amino and carboxyl termini by dibasic residues. GLP-1 is a 37 amino acids peptide and produced in the small intestine and in the pancreas in the human, in either C-terminal-amidated on glycine-extended form. GLP1 (7-36) amide and its receptor are present in several brain regions and may play a role in the physiological control of feeding. Several reports have been presented as follows as to the biological activities of GLP-1. GLP-1 (7-37) and (7-36) amide is known as one of the most potent insulin secretagogues.
Yanaihara Institute Inc. developed a quantitative EIA kit with high specificity and sensitivity (detection limit 0.206ng/mL) for rat/mouse/human GLP-1 (YK160) as a useful tool for these necessaries.


Quantitative determination kits for Biomarker Flyer download [PDF]

Features

This kit recognizes Total GLP-1(PDF)PDF

  • The assay kit can measure Rat, Mouse&Human GLP-1 in the range of 0.206 - 50 ng/mL.
  • The assay completes within 16-18 hr.+1.5 hr.
  • With one assay kit, 41 samples can be measured in duplicate.
  • Test sample: plasma (rat/mouse/human)
    Sample volume: 30 µL
  • The 96-well plate in kit was consisted by 8-wells strips. The kit can be used separately.
  • Precision and reproducibility
    (Rat plasma)
    Intra-assay CV(%) 5.36 - 6.60
    Inter-assay CV(%) 5.51 -18.87
    (Human plasma)
    Intra-assay CV(%) 4.69 -10.67
    Inter-assay CV(%) 9.63 - 17.57
  • Stability and Storage
    Store all of the components at 2-8.
    12 months from the date of manufacturing.
    The expiry date is described on the label of kit.

<Specificity>
The EIA kit has high specificity to rat/mouse/human GLP-1 and shows cross reactivity neither rat/human/mouse glucagons, human glicentin nor rat/mouse/human GLP-2.

<Analytical recovery>
Rat plasma 89.20-110.4%, Human plasma 86.0-101.7%

Component

    Form Quantity Main Ingredient
1. Antibody coated plate MTP*1 1 plate (96 wells) Goat anti rabbit IgG
2. GLP-1 standard
(25ng/vial)
lyophilized 1 vial Synthetic GLP-1 (7-36) amide
3. Labeled antigen lyophilized 1 vial Biotinylated GLP-1 (7-36) amide
4. GLP-1 antibody liquid 1 bottle (6 mL) Rabbit anti-GLP-1 (7-36) amide
5. SA-HRP liquid 1 tube (0.2 mL) HRP labeled streptoavidin
6. Diluent for SA-HRP liquid 1 bottle (12 mL) Phosphate buffer
7. Substrate buffer liquid 1 bottle (26 mL) 0.015% Hydrogen Peroxide
8. OPD tablet tablet 2 tablets o-Phenylenediamine dihydrochloride
9. Stopping solution liquid 1 bottle (10 mL) 2N H2SO4
10. Buffer solution liquid 1 bottle (10 mL) Phosphate buffer
11. Washing solution
(Concentrated)
Liquid 1 bottle (50 mL) Concentrated saline
12. Adhesive foil   3 sheets  

MTP*1 ….. Microtiter plate

Product List

Product Name Cat# Quantity Price

GlucagonLike Peptide 1 EIA KIT DataSheet ,

YII-YK160-EX

1 KIT ¥76,000
$1014

Related Products[Kit]


Product Name Cat# Quantity Price

Glucagon EIA DataSheet ,

YII-YK090-EX

1 KIT ¥76,000
$1014

Glicentin, EIA, Rat, Unlabeled DataSheet

YII-YK111-EX

1 KIT ¥76,000
$1014

Glucagon-Like Peptide 2 EIA, Human DataSheet

YII-YK141-EX

1 KIT ¥76,000
$1014

Glucagon-Like Peptide 2 EIA, Rat DataSheet

YII-YK140-EX

1 KIT ¥76,000
$1014

Glucagon-Like Peptide 2 EIA, Mouse DataSheet

YII-YK142-EX

1 KIT ¥76,000
$1014

Related Products[antibody]


Product Name Cat# Quantity Price

Anti Total Glucagon, Human (Rabbit) Unlabeled DataSheet

YII-YP040-EX

50 UL ¥40,000
$534

Antigen : Total Glucagon
IHC : 1:1,000-5,000 (frozen section)
RIA : 1:80,000
Specificity :
Pancreatic glucagon 100%, glicentin (porcine) 100%, glucagon (1-12) < 0.001% glucagon (13-17) <0.001%,
glucagon (19-29) 0%, secretin (porcine) 0%, VIP (porcine) 0%


Product Name Cat# Quantity Price

Anti GLP 1 (7-36)amide, Human (Rabbit) Unlabeled DataSheet

YII-Y320-EX

50 UL ¥35,000
$467

Antigen : GLP-1 (7-36)-NH2
IHC : 1:1000-4,000
EIA : 1:8,000
RIA : 1:21,000
Specificity :
Base on EIA: GLP-1 (7-36)-NH2 100%, GLP-1(1-37)-OH<0.01%, GLP-1(7-37)-OH<0.01%
GLP-1(1-36)-NH2 0.31%, GLP-2 (rat) < 0.1%, GLP-2 (human) < 0.1%
glicentin (human) < 0.1%, glucagon < 0.1%


Product Name Cat# Quantity Price

Anti GLP 2 (14-33), Rat (Rabbit) Unlabeled DataSheet

YII-Y321-EX

50 UL ¥35,000
$467

Antigen : GLP-2 (14-33)(Rat)
IHC : 1:1,000-5,000
RIA : 1:10,500
Specificity : GLP-2 (14-33) (rat) 100%, GLP-2 (rat) 100%, GLP-1 (7-36)-NH2(rat) 0%, glucagon 0%


Product Name Cat# Quantity Price

Anti GLP 2, Rat (Rabbit) Unlabeled DataSheet

YII-Y322-EX

50 UL ¥35,000
$467

Antigen : GLP-2 (Rat)
IHC : 1:500-2,000
EIA : 1:3,000-50,000
Specificity :
Base on EIA: : GLP-2 (rat) 100%, GLP-2 (human) 100%, GLP-2 (3-33) (rat) 90%
GLP-2 (14-33) (rat) 36%, GLP-2 (1-18) (rat) < 0.1%, GLP-1(7-36)-NH2(rat) 0%
glicentin (rat) 0%


Product Name Cat# Quantity Price

Anti GLP-2, Mouse (Rabbit) Unlabeled DataSheet

YII-Y323-EX

50 UL ¥35,000
$467

Antigen : GLP-2 (Mouse)
IHC : 1:1,000- 5,000
EIA : 1:10,000-60,000
Specificity :
Based on competitive EIA: GLP-2 (mouse) 100%, GLP-2 (rat) 100%, GLP-2 (14-33) (rat) 117.4%
GLP-2 (human) 44.7%, GLP-2 (1-18) (rat) 0%, GLP-1 (7-36)-NH2 0%


Product Name Cat# Quantity Price

Anti Glicentin (1-32), Rat (Rabbit) Unlabeled DataSheet

YII-Y324-EX

50 UL ¥35,000
$467

Antigen : Glicentin (1-32) (Rat)
IHC : 1:1,000- 5,000
EIA : 1:5,000-25,000
Specificity :
Based on competitive EIA: Glicentin (1-32) (rat) 100%, Glicentin (rat) 100%
Glicentin (human) 0.2%, Glucagon < 0.1%, GLP-1 < 0.1% , GLP-2 (rat) < 0.1%


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