useful for quantitative determination of rat leptin in its plasma, serum & culture supernatant samples

Rat Leptin ELISA Kit


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Background

Leptin, which is a product of ob gene, is a protein consisting of 167 amino acids and it is secreted from white adipose tissue. It is known that leptin acts on hypothalamus to decrease food intake and to reduce body weight, body fat, blood sugar and blood insulin in a healthy and an ob/ob mouse. Further, gene expression of neuropeptide Y(NPY) is surpressed by leptin. Recently, radioimmunoassay for leptin determination in human plasma has become available and leptin level in human patient group with obesity was found to increase in comparison with that of normal group. The level well correlate with body fat and these observations show clearly that leptin concentration in human plasma reflects the tissue fat weight. The measurement of plasma leptin may be an excellent index of obesity. Although rat leptin shows a high homology(96%) with mouse leptin, it is observed that substitution of several amino acid residues occurs at both end N- and C- terminal region between human and rat leptin. These findings have required urgently to develop highly sensitive immunoassay system specific to rat leptin. Yanaihara Insutitute Inc. has developed the enzyme immunoassay (EIA) kit which is a stable and convenient assay system for rat leptin in its plasma, serum and culture supernatant.

YII-YK050-EX "Rat Leptin ELISA KitPDF


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Features

This ELISA kit is used for quantitative determination of rat leptin in its plasma, serum & culture supernatant samples. It has a lot of advantage to perform the assay, such as good quantification, no influence with other body fluid factors or physiological active substances and needlessness of sample pretreatment.

  • The assay kit can measure Leptin in the range of 312.5-20,000pg/mL (serum or plasma) and 78.1-5,000pg/ml (non-plasma & non-serum sample, culture supernatant)
  • The assay completes within 5.5 hours.
  • With one assay kit, 40 samples can be measured in duplicate
  • Test sample : Serum,plasma or non-plasma & non-serum sample, culture supernatant
        Sample volume: plasma or serum 20 μL
        Non-plasma & non-serum sample, culture supernatant 50 μL
  • The 96-well plate in kit was consisted by 8-wells strips. The kit can be used separately.
  • Precision and reproducibility
        Intra-assay CVi“jSerum 3.9 - 4.5
        Inter-assay CVi“jSerum 6.2 - 9.5
  • Stability and storage  Store all the components at 2-8°C.
    The kit is stable under the condition 8 months from the date of manufacturing.

<Specificity>
The EIA kit has high specificity to rat leptin and shows less than 0.02% – 0.04% cross reactivity to human Leptin. And it has no cross reactivity with rat IL-1α, IL-1β, rat TNF-α, human TNF-α and other cytokines.

<Test Principle>
This EIA kit for determination of rat leptin in plasma, serum and culture supernatant samples is based on a sandwich enzyme immunoassay. The 96 wells plate is coated with anti rat leptin monoclonal antibody. Rat leptin standard or samples and HRP-labeled anti rat leptin polyclonal antibody are added to the wells for one step sandwich immunoreaction. During this immunoreaction, monoclonal antibody – antigen –HRP labeled antibody complex are formed. After rinsing out excess HRP labeled antibody, HRP enzyme activity is determined and the concentration of rat leptin is calculated.

Composition

Component Form Quantity Main Ingredient
1. Antibody coated plate MTP*1 1plate
(96 wells)
Anti rat Leptin monoclonal antibody
2. Rat Leptin standard lyophilized 1 vial Rat Leptin (20ng)
3. HRP labeled antibody liquid 1 bottle
(6 mL)
HRP labeled antibody from rabbit
4. Substrate buffer liquid 1 bottle
(24 mL)
0.015% Hydrogen Peroxide
5. OPD tablet tablet 2 tablets o-Phenylenediamine hydrochloride
6. Stopping solution liquid 1 bottle
(12 mL)
2N-H2SO4
7. Buffer solution A liquid 1 bottle
(20 mL)
Phosphate buffer including animal serum
8. Buffer solution B liquid 1 bottle
(15 mL)
Phosphate buffer including surfactant
9. Washing solution
(concentrated)
liquid 1 bottle
(50 mL)
Concentrated saline
10.Adhesive foil   2 sheets  

MTP*1 …. . Microtiration plate

Product List

Product Name Cat# Quantity Price

Leptin ELISA, Rat DataSheet

YII-YK050-EX

1 KIT ¥60,000
$800

Related Products [ Kit ]


Product Name Cat# Quantity Price

Leptin, ELISA (High Sensitivity), Rat DataSheet

YII-YK051-EX

1 KIT ¥60,000
$800

Leptin, ELISA Kit, Mouse DataSheet

YII-YK052-EX

1 KIT ¥60,000
$800

Related Products [ antibody ]


Product Name Cat# Quantity Price

Anti Leptin, Rat (Rabbit) Unlabeled DataSheet

YII-YC040-EX

50 UL ¥60,000
$800

Antigen : Leptin (Rat)
IHC : 1:4,000
RIA(final dilution) : 1:21,000
Specificity : Leptin(rat) 100%, leptin(human) 10%


Product Name Cat# Quantity Price

Anti TNF α, Rat (Rabbit) Unlabeled DataSheet

YII-YC030-EX

50 UL ¥60,000
$800

Antigen : TNF-α(Rat)
IHC : 1:10,000
RIA(final dilution) : 1:1,000
Specificity :
TNF-α(rat) 100%, TNF-α(human) 26.2%, IL-1α(rat) <0.01%,, IL-1α(human) < 0.01%,
IL-1β(rat) <0.01%, IL-1β(human) <0.01%, other cytokines <0.01%


Product Name Cat# Quantity Price

Anti TNF α, Rat (Goat) Unlabeled DataSheet

YII-YC031-EX

50 UL ¥60,000
$800

Antigen : TNF-α(Rat)
IHC : 1:1,000
RIA(final dilution) : 1:1,000
Specificity :
TNF-α(rat) 100%, TNF-α(human) 35.7%, IL-1α(rat) <0.01%, IL-1α(human) < 0.01%,
IL-1β(rat) <0.01%, IL-1β(human) <0.01%, other cytokines <0.01%


Product Name Cat# Quantity Price

Anti TNF α, Rat (Mouse) Unlabeled DataSheet

YII-YC032-EX

50 UL ¥60,000
$800

Antigen : TNF-α(Rat)
IHC : 1:1,000
RIA(final dilution) : 1:1,000
Specificity :
TNF-α(rat) 100%, TNF-α(human) <0.05%, IL-1α(rat) <0.05%, IL-1α(human) < 0.05%,
IL-1β(rat) <0.05%, IL-1β(human) <0.05%, other cytokines <0.05%


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