useful for quantitative determination of mouse C-Peptide in its plasma, serum & urine samples.

Mouse C - Peptide I EIA Kit


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Background

This enzyme immunoassay (EIA)kit is a stable and convenient assay system for mouse C-peptide I in its plasma, serum and urine.
The processing of proinsulin, which occurs within the B cell, yields insulin and C-peptide and insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of C-peptide in blood reflects the concentration of insulin and also provides valuable information to evaluate the pancreatic B cell function.
The EIA kit is prepared by using synthetic mouse C-Peptide I as standard antigen and biotinylated mouse C-Peptide I as labeled antigen. The kit contains specific polyclonal antibody recognized to the amino acid sequence of mouse C-Peptide.
Now, we have still tried to develop mouse C-peptide II EIA kit and mouse C-peptide I+II kit in our laboratory.


Quantitative determination kits for Biomarker Flyer download [PDF]

Feature and Advantages

This ELISA kit is used for quantitative determination of mouse C-Peptide in its plasma, serum & urine samples. It has a lot of advantage to perform the assay, such as good quantification, no influence with other body fluid factors or physiogical active substances and needlessness of sample pretreatment. Mouse C-Peptide I standard is highly purified synthetic product ( purity: higher than 98% ) and biotinylated peptide is stable because N –biotinylglycylglycyl Mouse C-Peptide I is used for it.

  • The assay kit can measure mouse C-Peptide in the range of 0.617`50 ng/mL
  • The assay completes within 16-18 hr.+ 2.5 hr.
  • With one assay kit, 42 samples can be measured in duplicate
  • Test sample: mouse serum, plasma, and urine Sample. volume: 25 μL
  • The 96-well plate in kit was consisted by 8-wells strips. The kit can be used separately.
  • Precision and reproducibility Intra-assay CV(%) Serum 3.1-4.9 Plasma 5.6-7.5 Urine 3.4-4.6 Inter-assay CV(%) Serum 4.7-8.8 Plasma 4.8-8.1 Urine 5.3-11.3
  • Stability and Storage Store all of the components at 2-8°C. 24 months from the date of manufacturing. The expiry date is described on the label of kit.

<Specificity>
The EIA kit shows each cross reactivity of 6.3% to mouse C-Peptide II, 6.5% to mouse insulin, 156.4% to rat C-Peptide I, 114.6% to rat C-Peptide II and 7.4% to rat insulin and shows no cross reactivity to human and dog C-Peptide.

<Test Principle>
This EIA kit for determination of mouse C-Peptide I in plasma and urine samples is based on a competitive enzyme immunoassay using combination with highly specific antibody to mouse C-Peptide I@and biotin – avidin affinity system. The 96 wells plate is coated with goat anti rabbit IgG and C-Peptide I standard or samples, biotinylated mouse C-Peptide I and rabbit anti mouse C-Peptide I antibody are added to the wells for competitive immunoreaction. After rinsing out excess mouse C-Peptide I, HRP labeled streptoavidins are added to bind to the antigen-antibody complex so that HRP labeled streptoavidin - biotinylated mouse C-Peptide – antibody complexs are formed on the surface on the wells. Finally, excess HRP labeled streptoavidins are rinsed out and HRP enzyme activity is determined and the concentration of mouse C-Peptide I is calculated.

Composition

ComponentFormQuantityMain@Ingredient
1. Antibody coated plate MTP*1 1 plate
(96 wells)
Goat anti rabbit IgG
2. C-Peptide standard lyophilized 1 vial
(50ng)
Synthetic mouse C-Peptide I
3. Labeled  antigen lyophilized 1 vial
(1.5 ng)
Biotinylated mouse C-Peptide I
4. C-Peptide antibody liquid 1 bottle
(6 mL)
Rabbit anti mouse C-Peptide I
5. SA-HRP solution liquid 1 bottle
(12 mL)
HRP labeled streptoavidin
6. Substrate buffer liquid 1 bottle
(26 mL)
0.015% Hydrogen Peroxide
7. OPD tablet tablet 2 tablets o-Phenylenediamine dihydrochloride
8. Stopping solution liquid 1 bottle
(12 mL)
1M H2SO4
9. Buffer solution
(concentrated)
Liquid 1 bottle
(20 mL)
Phosphate buffer
10. Washing solution
(concentrated)
Liquid 1 bottle
(50 mL)
Concentrated saline
11. Adhesive foil   3 sheets  

MTP*1........ Microtitration plat

Product List

Product Name Cat# Quantity Price

C-peptide1 EIA, Mouse DataSheet

YII-YK011-EX

1 KIT ¥76,000
$1014

Species : MS
Assay range : 0.617 ~ 50 ng/mL (Detection Limit 0.206ng/mL)
Assay sample volume : 25 µL (Serum, Plasma, Urine)
Assay time : 16-18 hr.+2.5 hr.

Related Products [ KIT ]


Product Name Cat# Quantity Price

C-Peptide EIA, Rat DataSheet

YII-YK010-EX

1 KIT ¥76,000
$1014

C-Peptide II, ELISA, Mouse DataSheet

YII-YK012-EX

1 KIT ¥76,000
$1014

C-Peptide EIA, Mouse DataSheet

YII-YK013-EX

1 KIT ¥76,000
$1014

YII-YK010-EX
Species : RAT
Assay range : 1.56 ~ 50 ng/mL
Assay sample volume : 50 µL (Culture Supernatant, Plasma, Serum, Urine)
Assay time : 5.5 hr.

YII-YK012-EX
Species :MS
Assay range : 0.412 ~ 100 ng/mL
Assay sample volume : 25 µL (Serum, Plasma, Urine)
Assay time : 16-18 hr.+2.5 hr.

YII-YK013-EX
Species : MS
Assay range : 0.412 ~ 100ng/mL
Assay sample volume : 25µL (Serum, Plasma)
Assay time : 18-20 hr.+1.5 h

Related Products [ antibody ]


Product Name Cat# Quantity Price

Anti C Peptide II, Rat (Rabbit) Unlabeled DataSheet

YII-Y220-EX

50 UL ¥35,000
$467

Antigen : C-Peptide I (Rat)
EIA : 1: 3,000
IHC : 1:500-1,000
RIA : 1:10,500
Specificity(Based on EIA) :
C-peptide I (rat) 100%, C-peptide II (rat) 52.9%, C-peptide I (mouse) 28.8% 
C-peptide II (mouse) 2.95%, C-peptide (human) 0%


Product Name Cat# Quantity Price

Anti C Peptide I, Rat (Rabbit) Unlabeled DataSheet

YII-Y221-EX

50 UL ¥35,000
$467

Antigen : C-Peptide II (Rat)
IHC : 1:1,000-10,000
RIA : 1:10,000
Specificity :
C-peptide I (rat) 100%, C-peptide II (rat) 100%,
C-peptide I (7-31) (rat) 100%, C-peptide II (7-31) (rat) 100%
C-peptide I (15-31) (rat) 100%, C-peptide II (15-31) (rat) 100%,C-peptide I (20-31) (rat) 80 %,
C-peptide I (1-23) (rat) <0.001%, C-peptide II (1-23) (rat) <0.001%, C-peptide (human) 0.01%


Product Name Cat# Quantity Price

Anti C Peptide I, Mouse (Rabbit) Unlabeled DataSheet

YII-Y222-EX

50 UL ¥35,000
$467

Antigen : C-Peptide I (Mouse)
IHC : 1:1,000- 5,000
Specificity (Based on non-competitive EIA) :
C-Peptide I (mouse) 100%, C-Peptide II (mouse) 15.2%, C-Peptide I (rat) 20.9% 
C-Peptide II (rat) 85%, C-Peptide (human) < 0.01%, C-Peptide (dog) < 0.01% 
C-Peptide (porcine) < 0.1%, Glucagon < 0.01%


Product Name Cat# Quantity Price

Anti C Peptide II, Mouse (Rabbit) Unlabeled DataSheet

YII-Y223-EX

50 UL ¥35,000
$467

Antigen : C-Peptide II (Mouse)
IHC : 1:2,000- 10,000
Specificity(Based on non-competitive EIA) :
C-Peptide II (mouse) 100%, C-Peptide I (mouse) 5.2%, C-Peptide I (rat) 3.7%, C-Peptide II (rat) 72.3%
C-Peptide (human) < 1.5%, C-Peptide (dog) < 0.01%, Glucagon < 0.1%, C-peptide (porcine) < 0.01%


“Mouse C - Peptide I EIA Kit” also belongs to the following categories.