useful for quantitative determination of mouse C-Peptide II in its plasma, serum & urine samples

Mouse C-Peptide II EIA Kit


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Background

This enzyme immunoassay (EIA) kit is a stable and convenient assay system for mouse C-peptide II in its plasma, serum and urine. The processing of proinsulin, which occurs within the B cell, yields insulin and C-peptide. The insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of C-peptide in blood reflects the concentration of insulin and also provides valuable information to evaluate the pancreatic B cell function.
The EIA kit is prepared by using synthetic mouse C-Peptide II as standard and biotinylated mouse C-Peptide II as labeled antigen. The kit contains specific polyclonal antibody recognized to the amino acid sequence of mouse C-Peptide II.
We have already developed mouse C-peptide I EIA kit and mouse C-peptide I+II kit is also been developing in our laboratory


Quantitative determination kits for Biomarker Flyer download [PDF]

Feature and Advantages

This ELISA kit is used for quantitative determination of mouse C-Peptide II in its plasma, serum & urine samples. The kit is characterized for sensitive quantification, high specificity and no influences with other components in samples. Mouse C-Peptide II standard is a highly purified synthetic product (purity: higher than 98%).

  • The assay kit can measure mouse C-Peptide II in the range of 0.412-100 ng/mL.
  • The assay completes within 16-18 + 2.5 hours.
  • With one assay kit, 41 samples can be measured in duplicate
  • Test sample: mouse plasma, serum or urine Sample volume: 25 μL
  • The 96-well plate in kit was consisted by 8-wells strips. The kit can be used separately.
  • Precision and reproducibility
      Intra-assay CV(%) Serum 4.12-5.66
      Inter-assay CV(%) Serum 2.72-10.62
  • Stability and Storage Store all of the components at 2-8°C.
      6 months from the date of manufacturing. The expiry date is described on the label of kit.

<Specificity>
The EIA kit shows following cross reactivity of 0.091% to mouse C-Peptide I, 0.34% to rat C-Peptide I, 63.3% to rat C-Peptide II and 1.8% to rat insulin and no cross reactivity to human and dog C-Peptide.

<Test Principle>
This EIA kit for determination of mouse C-Peptide II in serum, plasma and urine samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to mouse C-Peptide II@and biotin-avidin affinity system. The 96 wells plate is coated with goat anti-rabbit IgG. C-Peptide II standard or samples, labeled antigen and rabbit anti mouse C-Peptide II antibody are added to the wells for competitive immunoreaction. After incubation and plate wash, HRP labeled streptoavidin (SA-HRP) are added to form HRP labeled streptoavidin-biotinylated mouse C-Peptide II-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of mouse C-Peptide II is calculated.

Composition

ComponentFormQuantityMain@Ingredient
1. Antibody coated plate MTP*1 1 plate
(96 wells)
Goat anti rabbit IgG
2. C-Peptide standard lyophilized 1 vial
(100 ng)
Synthetic mouse C-Peptide II
3. Labeled  antigen lyophilized 1 vial Biotinylated mouse C-Peptide II
4. C-Peptide II antibody liquid 1 bottle
(6 mL)
Rabbit anti mouse C-Peptide II
5. SA-HRP solution liquid 1 bottle
(12 mL)
HRP labeled streptoavidin
6. Substrate buffer liquid 1 bottle
(26 mL)
0.015% Hydrogen Peroxide
7. OPD tablet tablet 2 tablets o-Phenylenediamine dihydrochloride
8. Stopping solution liquid 1 bottle
(12 mL)
1M H2SO4
9. Buffer solution
(concentrated)
Liquid 1 bottle
(25 mL)
Phosphate buffer
10. Washing solution
(concentrated)
Liquid 1 bottle
(50 mL)
Concentrated saline
11. Adhesive foil   3 sheets  

MTP*1........ Microtitration plat

Product List

Product Name Cat# Quantity Price

C-Peptide II, ELISA, Mouse DataSheet

YII-YK012-EX

1 KIT ¥76,000
$1014

Species : MS
Assay range : 0.412 ~ 100 ng/mL
Assay sample volume : 25 µL(Serum, Plasma, Urine)
Assay time : 16-18 hr.+2.5 hr.

Related Products [ KIT ]


Product Name Cat# Quantity Price

C-Peptide EIA, Rat DataSheet

YII-YK010-EX

1 KIT ¥76,000
$1014

C-peptide1 EIA, Mouse DataSheet

YII-YK011-EX

1 KIT ¥76,000
$1014

C-Peptide EIA, Mouse DataSheet

YII-YK013-EX

1 KIT ¥76,000
$1014

YII-YK010-EX
Species : RAT
Assay range : 1.56 ~ 50 ng/mL
Assay sample volume : 50 µL (Culture Supernatant, Plasma, Serum, Urine)
Assay time : 5.5 hr.

YII-YK011-EX
Species :MS
Assay range : 0.617 ~ 50 ng/mL(Detection Limit 0.206ng/mL)
Assay sample volume : 25 µL(Serum, Plasma, Urine)
Assay time : 16-18 hr.+2.5 hr.

YII-YK013-EX
Species : MS
Assay range : 0.412 ~ 100ng/mL
Assay sample volume : 25µL (Serum, Plasma)
Assay time : 18-20 hr.+1.5 h

Related Products [ antibody ]


Product Name Cat# Quantity Price

Anti C Peptide II, Rat (Rabbit) Unlabeled DataSheet

YII-Y220-EX

50 UL ¥35,000
$467

Antigen : C-Peptide I (Rat)
EIA : 1: 3,000
IHC : 1:500-1,000
RIA : 1:10,500
Specificity(Based on EIA) :
C-peptide I (rat) 100%, C-peptide II (rat) 52.9%, C-peptide I (mouse) 28.8% 
C-peptide II (mouse) 2.95%, C-peptide (human) 0%


Product Name Cat# Quantity Price

Anti C Peptide I, Rat (Rabbit) Unlabeled DataSheet

YII-Y221-EX

50 UL ¥35,000
$467

Antigen : C-Peptide II (Rat)
IHC : 1:1,000-10,000
RIA : 1:10,000
Specificity :
C-peptide I (rat) 100%, C-peptide II (rat) 100%,
C-peptide I (7-31) (rat) 100%, C-peptide II (7-31) (rat) 100%
C-peptide I (15-31) (rat) 100%, C-peptide II (15-31) (rat) 100%,C-peptide I (20-31) (rat) 80 %,
C-peptide I (1-23) (rat) <0.001%, C-peptide II (1-23) (rat) <0.001%, C-peptide (human) 0.01%


Product Name Cat# Quantity Price

Anti C Peptide I, Mouse (Rabbit) Unlabeled DataSheet

YII-Y222-EX

50 UL ¥35,000
$467

Antigen : C-Peptide I (Mouse)
IHC : 1:1,000- 5,000
Specificity
(Based on non-competitive EIA) :
C-Peptide I (mouse) 100%, C-Peptide II (mouse) 15.2%, C-Peptide I (rat) 20.9% 
C-Peptide II (rat) 85%, C-Peptide (human) < 0.01%, C-Peptide (dog) < 0.01% 
C-Peptide (porcine) < 0.1%, Glucagon < 0.01%


Product Name Cat# Quantity Price

Anti C Peptide II, Mouse (Rabbit) Unlabeled DataSheet

YII-Y223-EX

50 UL ¥35,000
$467

Antigen : C-Peptide II (Mouse)
IHC : 1:2,000- 10,000
Specificity(Based on non-competitive EIA) :
C-Peptide II (mouse) 100%, C-Peptide I (mouse) 5.2%, C-Peptide I (rat) 3.7%, C-Peptide II (rat) 72.3%
C-Peptide (human) < 1.5%, C-Peptide (dog) < 0.01%, Glucagon < 0.1%, C-peptide (porcine) < 0.01%


“Mouse C-Peptide II EIA Kit” also belongs to the following categories.