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useful for quantitative determination of total mouse C-peptide, i.e. C-peptide I+II, in its serum and plasma samples.

Mouse C-Peptide EIA Kit and related products


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Background

This enzyme immunoassay (EIA) kit is a stable and convenient assay system for mouse C-peptide (mouse C-peptide I+II) in its serum and plasma.
The processing of proinsulin, which occurs within the@pancreatic B cell, yields insulin and C-peptide. The insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of C-peptide in blood reflects the concentration of insulin and also provides valuable information to evaluate the pancreatic B cell function.
This EIA kit includes synthetic mouse C-peptide II as standard antigen and biotinylated mouse C-peptide II as labeled antigen. The kit also contains specific polyclonal antibody recognizing mouse C-peptide I and mouse C-peptide II equivalently.
We have already developed EIA kits which can measure specifically mouse C-peptide I (YK011) and C-peptide II (YK012), respectively and now mouse C-peptide (I+II) kit (YK013) is developed for measuring C-peptide I and C-peptide II together. Namely this kit is useful for measuring total C-peptide level in mouse blood


Quantitative determination kits for Biomarker Flyer download [PDF]

Feature and Advantages

This EIA kit is used for quantitative determination of total mouse C-peptide, i.e. C-peptide I+II, in its serum and plasma samples. The kit is characterized by its sensitive quantification and high specificity. In addition, it is not influenced by other constituents in samples. Standard antigen, mouse C-peptide II of this kit is a highly purified synthetic product (purity: higher than 98%).

  • This assay kit can measure mouse C-peptide (I+II) within the range of 0.412-100 ng/mL
  • The assay is completed within 18-20 hr + 1.5 hr.
  • With one assay kit, 41 samples can be measured in duplicate ETest sample: mouse plasma and serum Sample volume: 25 μL
  • The 96-well plate of this kit consists of 128-wells strips, so that divided use by the strips is possible at user's option.
  • Precision and reproducibility Intra-assay CVi“j: Serum 1.4-3.1 Inter-assay CVi“j: Serum 4.2-8.1
  • Stability and Storage Store all of the components at 2-8°C. 6 months from the date of manufacturing. The expiry date is described on the label of kit.

<Specificity>
This EIA kit shows 93.9% crossreactivity to mouse C-peptide I, 100% to mouse C-peptide II, 22.9% to rat C-peptide and 48.2% to rat C-peptide II. It shows below 2% crossreactivity to mouse insulin and no crossreactivity to human, dog and pig C-peptide.

<Principle>
This EIA kit for determination of mouse C-peptide in serum and plasma is based on a competitive enzyme immunoassay using combination of highly specific antibody to mouse C-peptide (I+II) and biotin-avidin affinity system. To the wells of the plate coated with goat anti rabbit IgG, labeled antigen, standard antigen or samples and rabbit anti mouse C-peptide antibody are added for competitive immunoreaction. After incubation and plate washing, horse radish peroxidase (HRP) labeled streptoavidin (SA) is added to form HRP labeled SA-biotinylated antigen-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by 3,3’5,5’-tetramethyl benzidine (TMB) and the concentration of mouse C-peptide is calculated.

Composition

QuantityMain@Ingredient  
1. Antibody coated plate MTP*1 1 plate
(96 wells)
Goat anti rabbit IgG antibody
2. Standard antigen lyophilized 1 vial
(50 ng)
Synthetic mouse C-peptide II
3. Labeled antigen lyophilized 1 vial Biotinylated mouse C-peptide II
4. Specific antibody liquid 1 bottle
(6 mL)
Rabbit anti mouse C-peptide antibody
5. SA-HRP solution liquid 1 bottle
(12 mL)
HRP labeled streptoavidin
6. Enzyme substrate solution liquid 1 bottle
(12 mL)
3,3’,5,5’-tetramethyl benzidine (TMB)
7. Reaction stopping solution Liquid 1 bottle
(12 mL)
1M H2SO4
8. Buffer solution liquid 1 bottle
(25 mL)
Tris-HCl/saline buffer
9. Washing solution
(concentrated)
Liquid 1 bottle
(50 mL)
Concentrated saline
10. Adhesive foil   3 sheets  

MTP*1........ Microtitration plat

Product List

Product Name Cat# Quantity Price

C-Peptide EIA, Mouse DataSheet

YII-YK013-EX

1 KIT ¥76,000
$1014

Related Products [ antibody ]


Product Name Cat# Quantity Price

Anti C Peptide I, Mouse (Rabbit) Unlabeled DataSheet

YII-Y222-EX

50 UL ¥35,000
$467

Anti C Peptide II, Mouse (Rabbit) Unlabeled DataSheet

YII-Y223-EX

50 UL ¥35,000
$467

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