• HOME
  • Products
  • Purification of IgG - Affinity Gel yAb-Capcher™z, Column yAb-Rapid PuRe™z, Spin Column yAb-Rapid SPiN™z

IgG Purification Tool Beyond Protein A and G

Purification of IgG - Affinity Gel yAb-Capcher™z, Column yAb-Rapid PuRe™z, Spin Column yAb-Rapid SPiN™z


print

Background

Ab-Capcher™ is "Protein A-R28" (Protein A variant) which is developed with ProteNova’s patent technology bound at high density to a supporting gel matrix offering many practical advantages for routine purification of rat and mouse IgG including broader subclass specificity and high capacity binding.

Ab-Rapid PuRE™ is a specially designed syringe-type column unit prepacked with AbCapcher offering maximum convenience for Ig purification.

Ab-Rapid SPiN™ is a convenient spin column prepacked with Ab-Capcher.

 

Protein A-R28 is an alkali-tolerant IgG-binding protein derived from protein A, which is developed with ProteNova’s patent technology. Protein A-R28 strongly binds to various species and subclasses of IgG, compared with Protein A and G. The coupling to resin (Ab-Capcher) provides an alkali-washable unique affinity medium with high binding capacity for immunoglobulin, which is useful for purification of human, rabbit, and mouse IgGs including mouse IgG1.Ab-Aapcher is also useful for immuno-precipitation experiments.

Feature and Advantages

◊ 5 times the binding capacity of Protein G
◊ Labile antibodies can be bound at neutral pH
◊ Low non-specific binding
◊ IgG puirty >95%

Binding Characteristics of Ab-Capcher
Species SubclassAb-CapProtein AProtein G
Mouse IgG1 ++++ + ++
IgG2a +++++ ++++ +
Rat IgG1 +++ - +
IgG2a -* - +
Goat IgGs ++++ - +
Chicken IgY - - -
Human IgG +++++ ++++ ++
Rabbit IgG +++++ ++++ ++

*Connection has not confirmed.

Characteristic

Composition : Highly cross-linked 4% agarose
Particle size : 45-165 um
Ligand : Alkali-+tolerant variant of Protein A (Protein A-R28) (E.coli)
Binding capacity (max.) : > 65mg human IgG / ml gel
Delivery conditions : 20% EtOH

Recommended conditions

< Example of suitable buffers for human IgG (-70 mg / ml gel) >
Binding buffer : 20 mM sodium phosphate buffer, 150 mM NaCl, pH 7.2
Elution buffer : 0.1 M Glycine-HCL, pH 2.8
Neutralization buffer : 1 M Tris ( add 1/20 vol of elution buffer)
Washing buffer : 0.1 N NaOH
< Example of suitable buffers for mouse IgG1 (-40 mg / ml gel) >
Binding buffer : 1.5 M Glycine-HCL, 3 M NaCl, pH 9.0
Elution buffer : 0.1 M Glycine-HCL, pH 2.8
Neutralization buffer : 1 M Tris ( add 1/20 vol of elution buffer)
Washing buffer : 0.1 N NaOH
# Phosphate buffered saline can be used as binding buffer
(in this case, binding capacity is approx 20 mg of mouse IgG1 / ml of the gel)

Application DATA

Binding of Human IgG
Binding capacities of Ab-Capcher and two protein A-resins were tested with human gamma globulin. Phosphate buffered saline (PBS) was used as the binding buffer. Ab-Capcher showed the highest binding capacity in affinity media tested (-70 mg human IgG / ml gel).

Binding of monoclonal mouse IgG1 at pH 9
Mouse ascites were precipitated with 50%-saturated
ammonium sulfate, dissolved and dialyzed with PBS. The fraction diluted 3-fold with 1.5 M Glycine / 3 M NaCl buffer (pH 9.0). was used to test binding capacity of Ab-Capcher For mouse IgG1. Ab-Capcher bound high amounts of mouse IgG1 at pH 9.0 as well as human IgG pH 7.0.

Binding of monoclonal mouse IgG1 with PBS
Fraction of 50%-saturated ammonium sulfate from mouse ascites was used to test binding capacity of Ab-Capcher and Protein G-agarose for mouse IgG1 under physiological conditions. PBS was used as binding buffer. Ab-Capcher bound larger amounts of mouse IgG1 about 5-times than conventional Protein G-agarose under physiplosical conditions ( 18.3 mg / ml gel ).

Alkali-arability
Relative binding capacities for human IgG of Ab-Capcher and conventional Protein A-agarose were measured after treatment with 0.1 N NaOH at 25Ž. Ab-Caocher remains 90 % of binding capacity after treatment for 106 hr at 25Ž.

 

< Experiment example data > EEE@coming soon

<Ab-Capcher FAQ> EEE@coming soon

Product List

Affinity Gel for Purification of IgG yAb-Capcher™z


Product Name Cat# Quantity Price

Ab-CapcherTM DataSheet ,

PTN-P-002-2

2 ML ¥25,000
$334

Ab-CapcherTM DataSheet ,

PTN-P-002-10

10 ML ¥75,000
$1000

Prepacked Column for Purification of IgGyAb-Rapid PuRe™z Basic specification


Product Name Cat# Quantity Price

Ab-Rapid Pure 2 DataSheet ,

PTN-P-012-2

2 COLUMN ¥16,000
$214

Ab-Rapid Pure 10 DataSheet ,

PTN-P-012-10

10 COLUMN ¥64,000
$854

Prepacked Spin Column for Purification of IgG yAb-Rapid SPiN™z


Product Name Cat# Quantity Price

Ab-Rapid SPiN10 DataSheet ,

PTN-P-013-10

10 PC ¥20,800
$278

Ab-Rapid SPiN50 DataSheet ,

PTN-P-013-50

50 PC ¥68,400
$912

Product Name Cat# Quantity Price

Buffer kit for Ab-Rapid Pure ,

PTN-P-011

10 RXN ¥5,000
$67

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



“Purification of IgG - Affinity Gel yAb-Capcher™z, Column yAb-Rapid PuRe™z, Spin Column yAb-Rapid SPiN™z” also belongs to the following categories.