Reproductive Engineering Manuals


 Cryopreservation of Mouse Spermatozoa
  (Using FERTIUP® Cryoprotectant, HTF, Sperm Straws, Straw connector and Freezing canister)

 In Vitro Fertilization using Cryopreserved Spermatozoa
  (Using FERTIUP® Preincubation medium, CARD MEDIUM®, HTF and Straw connector)

 Collection and Transport at Cold Temperature of Cauda Epididymis
  (Using Card Cold Transport Kit)

 In Vitro Fertilization using Epidydimal Sperm Transported at Cold Temperature
  (Using FERTIUP® Preincubation Medium: PM and HTF)

 Transport of 2-Cell Embryos at Cold Temperature
  (Using Card Cold Transport Kit, M2 and KSOM)

 In Vitro Fertilization (IVF) 
  (Using FERTIUP® Preincubation medium, CARD MEDIUM® and HTF)

 Simple Vitrification of Mouse Embryos
  (Using DAP213 and DMSO)

 Embryo Transfer into the Oviduct

 Rescue In Vitro Fertilization Method for Legacy Stock of Cryopreserved Spermatozoa
  (Using FERTIUP® Preincubation medium: PM, CARD MEDIUM® and HTF)

 Procedure for Warming of Vitrified Embryos
  (Using 0.25M sucrose and KSOM)

 Storage of Media and Solutions in Ampoules Under Nitrogen Gas

 Simple Vitrification of Mouse Oocytes
   (Using HTF)

 Tables of Media Composition
   1M DMSO, DAP213, 0.25M Sucrose, mHTF and KSOM


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To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.

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