Ribose-gelatin


print

Gelatin (2 mg/ml) was incubated with 30 mM of ribose in a 0.2 M phosphate buffer (pH 7.4) at 37°C for 1 week, followed by dialysis against PBS.

Fig.1 Immunoreactivity of the CMA(3F5) monoclonal antibody to Ribose-gelatin and Ribose-HSA

ELISA Protocol

ELISA protocol Coating
1) Distribute 100 ul / well of the sample solution (1 ug/mL in PBS) to 96 well microtiter plates (Thermo, MaxiSorp).
2) Incubate the plates 2h at RT or overnight at 4 degrees.
3) Discard the supernatant of sample solution.
4) Wash the plates three times with washing.buf.(PBS/0.05%Tween 20)

Blocking
1) Distribute 200 ul / well of 0.5% gelatin-PBS to 96 well microtiter plates
2) Incubate the plates 1h at RT.
3) Discard the the supernatant of 0.5% gelatin-PBS 4) Wash the plates three times with washing.buf.(PBS/0.05%Tween 20)

Primary antibody
1) Distribute 100 ul / well of Primary antibodies diluted with washing buf. as suggested in the APPLICATIONS to each well.
2) Incubate the plates 1h at RT.
3) Discard the supernatant of Primary antibody solution.
4) Wash the plates three times with washing.buf.(PBS/0.05%Tween 20)

Secondary antibody
1) Distribute 100 ul / well of secondary antibodies (HRP-anti mouse IgG) diluted with washing buf. as suggested in the APPLICATIONS to each well.
2) Incubate the plates 1h at RT.
3) Discard the supernatant of secondary antibody.
4) Wash the plates three times with washing.buf.(PBS/0.05%Tween 20)

OPD color reaction
1) Reaction for 2-10 minutes at RT.
2) Distribute 100 uL / well of 2M H2SO4 to each well and stop enzyme reaction.
3) After gentle mixing, determine the absorbance at 492 nm of each well by a spectrophotometer.

References

> Shimasaki S, Kubota M, Yoshitomi M, Takagi K, Suda K, Mera K, Fujiwara Y, Nagai R. NƒÖ-(carboxymethyl)arginine Accumulates in Glycated Collagen and Klotho-deficient Mouse Skin. Anti-Aging Medicine 8 (6) : 82-87, 2011 ANTI-AGING MEDICINE 8(6), 82-87, 2011

Product List

Product Name Cat# Quantity Price

Ribose-gelatin DataSheet ,

CSR-AGE-GP04

500 UL ¥20,000
$267

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


“Ribose-gelatin” also belongs to the following categories.