Minimize damage to your cells! 
in vitro/in vivo Transfection Reagent

SAFETRANS


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Background

SAFETRANS is an unique gene carrier composed of dendrimers (cationic polymers) and cyclic oligosaccharides, capable of in vitro and in vivo delivery of pDNA, shRNA, siRNA, hRNA and miRNA to a variety of cells with minimal cell toxicity.

[This reagent was developed by Dr. Hidetoshi Arima from Kumamoto University]

Principle of SAFETRANS

Transfection is carried out by the principle of polyfection as the target gene and dendrimer (cationic polymer) complex is introduced into cells by endocytosis. Dendrimers with many primary amino groups interact electrostatically with negatively charged nucleic acids and cell surface leading to efficient release of nucleic acid from endosomes due to the proton sponge effect. High gene induction is possible by complex formation with the cyclic oligosaccharides, resulting in a stronger interaction between dendrimers and cell/endosome membrane.  

Feature and Advantages

• Capable of transfection of pDNA, shRNA, siRNA, shRNA, and miRNA
• Capable of in vivo transfection by intravenous and subcutaneous injection
• Compatible for reverse transfection (α-CDE only)
• Successful results with many cell lines
• Low Cell Cytotoxity
• Uniform Gene Induction Rate
• Protocol requires simple adjustments
• Hepatocytes can be selectively transfected.

Compatible Cell Lines

• A549
• NIH3T3
• Colon-26
• HepG2
• NR8383
• RAW264.7
• KB

References

1. Kihara F, Arima H, Tsutsumi T, Hirayama F, Uekama K. In Vitro and In Vivo Gene Transfer by an Optimized ƒ¿-Cyclodextrin Conjugate with Polyamidoamine Dendrimer. Bioconjug. Chem., 14, 342-350, 2003.
2. Kihara F, Arima H, Tsutsumi T, Hirayama F, Uekama K. Effects of Structure of Polyamidoamine Dendrimer on Gene Transfer Efficiency of the Dendrimer Conjugate with ƒ¿-Cyclodextrin. Bioconjug. Chem., 13, 211-1219, 2002.
3. Arima H, Kihara F, Hirayama F, Uekama K. Enhancement of Gene Expression by Polyamidoamine Dendrimer Conjugates with ƒ¿-, ƒÀ-, ƒÁ-Cyclodextrins. Bioconjug. Chem., 12, 476-484, 2001.
4. Tsutsumi T, Hirayama F, Uekama K, Arima H. Potential use of Polyamidoamine Dendrimer/ƒ¿-Cyclodextrin Conjugate (generation 3, G3) as a Novel Carrier for Short Hairpin RNA-Expressing Plasmid DNA. J. Pharm. Sci., 97, 3022-3034, 2008.
5. Tsutsumi T, Hirayama F, Uekama K, Arima H. Evaluation of polyamidoamine dendrimer/ƒ¿-cyclodextrin conjugate (generation 3, G3) as a novel carrier for small interfering RNA (siRNA). J. Control. Release, 119, 349-359, 2007.
6. Tsutsumi T, Hirayama F, Uekama K, Arima H. Potential use of polyamidoamine dendrimer/ƒ¿-cyclodextrin conjugate (generation 3, G3) as a novel carrier for short hairpin RNA-expressing plasmid DNA, J. Pharm. Sci., 97, 3022-3034, 2008.
7. Anno T, Motoyama K, Higashi T, Hirayama F, Uekama K, Arima H. Preparation and evaluation of polyamidoamine dendrimer (G2)/branched-ƒÀ-cyclodextrin conjugate as a novel gene transfer carrier. J. Incl. Phenom. Macrocycl. Chem., in press, 2010.

Product List

Product Name Cat# Quantity Price

SAFETRANS ((α)-CDE) DataSheet ,

CSR-KMU-T01

2 MG N/A

SAFETRANS (GUG-(β)-CDE) DataSheet ,

CSR-KMU-T02

2 MG N/A

SAFETRANS (Lac-(α)-CDE) DataSheet ,

CSR-KMU-T03

2 MG N/A

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.