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Calcium-Binding Chaperones for Sperm Fertility


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Background

A large amount of cases of unexplained infertility in human males results from poor sperm quality. Calcium-binding chaperones such as Calsperin, Calmegin, PDILT and Calnexin are secondary messengers in the signal transduction pathway and play a major role in quality control by retaining incompletely folded or misfolded proteins in the endoplasmic reticulum (ER).

Calsperin/CALR3
-lectin chaperone located in the endoplasmic reticulum
-defect in Calsperin gene leads to defective sperm migration and defective binding to the egg extracellular matrix (zona pellucida) 2

Calnexin/ CANX
-an integral membrane protein
-lectin chaperone located in the endoplasmic reticulum

Calmegin
-A testis-specific ER protein that is homologous to calnexin
-Specifically expressed during male meiotic germ cell development.
-Defect in Calmegin leads to a defect where sperm cannot adhere to egg extracellular matrix (zona pellucida)
-Functions as a chaperone for one or more sperm surface proteins that mediate the interactions between sperm and egg1

PDILT
-Cooperates with the testis-specific calreticulin-like chaperone, calsperin (CALR3), in the endoplasmic reticulum
-Plays an indispensable role in the disulfide-bond formation and folding of ADAM3 (a gene which codes for a cysteine-rich transmembrane protein expressed in the testis).
-Pdilt(-/-) mice were male infertile because ADAM3 could not be folded properly and transported to the sperm surface without the PDILT/CALR3 complex.

 

 

1Masahito Ikawa et al., The putative chaperone calmegin is required for sperm fertility Nature 387, 607-611
2Masahito Ikawa et al., Calsperin Is a Testis-specific Chaperone Required for Sperm Fertility J Biol Chem. Feb 18, 2011; 286(7): 5639–5646

References

# Ikawa M., et al. Calmegin is required for fertilin alpha/beta heterodimerization and
sperm fertility. Dev Biol. 2001 Dec 1;240(1):254-61. PMID: 11784061
# Ikawa M., et al. Calsperin is a testis-specific chaperone required for sperm fertility.
J Biol Chem. 2011 Feb 18;286(7):5639-46. PMID: 21131354
> Okiyoneda T, Wada I, Jono H, Shuto T, Yoshitake K, Nakano N, Nagayama S,
Harada K, Isohama Y, Miyata T, Kai H. Calnexin Delta 185-520 partially reverses the misprocessing of the Delta F508 cystic fibrosis transmembrane conductance regulator.
FEBS Lett. 2002 Aug 28;526(1-3):87-92.
> Okiyoneda T, Kai H. Introduction of the conventional method of DNA transfection by adenovirus vector Nippon Yakurigaku Zasshi. 2002 Jun;119(6):337-44. Japanese.

Product List

Product Name Cat# Quantity Price

Anti Calreticulin-3/CALR3/Calsperin (Rabbit)  DataSheet

BAM-73-022-EX

100 UL ¥34,500
$460

Anti Calreticulin/CALR (Rabbit)  DataSheet

BAM-73-018-EX

100 UL ¥34,500
$460

Anti Calmegin, Mouse (Rabbit)  DataSheet

BAM-73-034-EX

100 UL ¥34,500
$460

Anti PDILT, Mouse (Rabbit)  DataSheet

BAM-73-051-EX

100 UL ¥34,500
$460

Anti Calnexin, Human (Mouse) Unlabeled DataSheet

KAL-KK054

50 UG ¥55,000
$734

Anti CNX1/2 Arabidopsis thaliana  (Rabbit) 

AGR-AS12 2365

50 UG ¥86,000
$1147

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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