SUMO Antibodies


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Background

SUMO is Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO1 involves for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2.


Ubiquitin, SUMO & Proteasome Antibodies flyer download [PDF]

Applications

Fig.1. Detection of SUMO-1 by Western blotting with the antibody 4D12. An 80kDa single and other multiple bands were observed in HeLa total cell extract. The 80 kDa band would be SUMO-RanGAP. Anti-SUMO-1 antibody 4D12 was used at 1 µg/ml.

 

    

Fig.2. Immunofluorescence staining of SUMO-1 with the antibody 4D12 in the mouse primary culture neurons. Left: Stained with anti-SUMO-1 antibody 4D12 at 10 µg/ml. Light: DNA was stained with Hoechst

Fig.3. SUMO1 colocalizes with SUMO-2/3 as revealed by indirect immunofluorescence staing of C-33A cells (human cervix carcinoma). Left: SUMO-1 was stained withanti-SUMO1 antibody (4D12) at 10 µg/ml. Middle: SUMO2/3 was stained with anti-SUMO2/3 antibody (3H12) at 10 µg/ml. Right: Merged image

   

Fig4. Immunocytochemistry/ Immunofluorescence - SUMO2/3 antibody (3H12) Mouse primary culture neuron

Fig5.Western blot - SUMO1 antibody (4D12) and SUMO3 antibody (3H12) HeLa cell total extracts

References

> Saitoh et al.,(2006). Exp Cell Res., 312, 1418-1430.
> Ulrich HD "The fast-growing business of SUMO chains.h Review Mol Cell 32: 301?305 (2008) PMID:18995828
> Cheng J et al gRole of desumoylation in the development of prostate cancer.h Review Neoplasia 8: 667-676 (2006) PMID: 16925949
> Uchimura Y et al gInvolvement of SUMO modification in MBD1- and MCAF1-mediated
heterochromatin formation.h J Biol Chem 281: 23180-23190 (2006) PMID: 16757475

Product List

Product Name Cat# Quantity Price

Anti SUMO1 (Rat) Unlabeled DataSheet

BAM-70-653-EX

100 UG ¥23,000
$307

Anti SUMO1, Human (Rat) Biotin DataSheet

BAM-70-654EX

50 UG ¥23,000
$307

Anti SUMO1, Human (Rat) Fluorescein Isothiocyanate DataSheet

BAM-70-655EX

50 UG ¥23,000
$307

Anti SUMO1 Arabidopsis thaliana  (Rabbit) 

AGR-AS08 308

1.5 MG ¥121,000
$1614

Anti SUMO 2/3, Human (Rat Mono)  DataSheet

BAM-70-657-EX

100 UG ¥29,000
$387

Anti SUMO2 and SUMO3, Human (Rat) Unlabeled DataSheet

CAC-CE-042A

100 UL ¥58,000
$774

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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