• HOME
  • Products
  • L-Glutamate Assay Kit IIL-Glutamate Oxidase (recombinant)

Useful tools for L-Glutamate measurement

L-Glutamate Assay Kit II
L-Glutamate Oxidase (recombinant)


print

L-Glutamate Assay Kit II

• ‚hntended use •

<For the food manufacturing industry>

1. Material control 
2. Manufacturing control 
3. Quality control

<For neuroscience and biology laboratories> 
L-glutamate is a critical neurotransmitter. 
L- glutamate has key roles in cell metabolism and disease.

 

• Characteristics •

1. Accurate quantification
Easy to use kit accurately measures L-glutamate in samples. No special skill is required.

2. Simple handling
  Simple protocol compared to older assays utilizing L-glutamate decarboxylase or L-glutamate dehydrogenase.

3. Low overhead cost
  Room temperature assay reads on standard absorption spectrometer at 600 nm. Does not require expensive specialized instrumentation.

 

• Kit Components •

1. Assay buffer: Good’s buffer, pH 7.1..................................1 bottle (60 mL) 
2. L-glutamate oxidase: lyophilized powder...........................1 vial 
3. Standard solution: L-glutamate solution (100 mg/L)...........1 vial (1.5 mL) 
4. Assay protocol

L-Glutamate Oxidase (recombinant)

Enzyme: L-Glutamate Oxidase (EC 1.4.3.11 )
Appearance: Lyophilized powder
Figure: 25 units/vial
Source: Recombinant E. coli

• Character •

Molecular weight 140,000
Subunit 2β2γ2iα:43,000, β:17,000, γ:10,000j
Thermo stability Stable below 60ºC (30min at pH 6)
pH stability pH 4 - 10 (60min at 50ºC)
Optimum pH pH 7 - 9
Km 0.2 mM
Substrate specificity L-Glu 100%
L-Gln 0%
L-Asp 0%
D-Glu 0%
0% for other 16 kinds of L-amino acids

• Storage •

Keep dry in a refrigerator (After dissolved the oxidase, it is preferable to store at low temperature (- 80°C). Repeated freeze-thaw cycles should be avoided.)

• Note •

This product is not suitable for L-Glutamate Assay Kit II (Cat. No. YMS-80057)

References

> Arima, J. et al., J.Biochem. 134, 805-812 (2003)
> Yamaguchi H. et. al., Nihon Shoyu Kenkyujo Zasshi (Japanese), 13, 8-12, 1987.
> Kusakabe H. et. al., Agric. Biol. Chem., 48, 181-184, 1984.

Product List

Product Name Cat# Quantity Price

L-Glutamate Assay Kit II DataSheet ,

YMS-80057

1 KIT see also
# YMS-80128
Size 1

L-Glutamate Oxidase 25 DataSheet ,

YMS-80049

25 UNIT ¥52,000
$694

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


“L-Glutamate Assay Kit IIL-Glutamate Oxidase (recombinant)” also belongs to the following categories.