Back Ground

In recent years, influence of Endocrine Disrupting Chemicals (EDC) upon living organisms has been one of the hot topics in the environmental science. However, evaluation methods of chemical compound species that may have EDC effects has not yet been established, and it is absolutely necessary to take action to complete such a task.
Vitellogenin (Vg), a precursor of yolk protein, is a female-specific protein that is present only in the blood of higher oviparous vertebrate organisms. Vitellogenin is normally expressed in the liver under regulation of estrogen (female sex hormone) during an egg formation process, and when it reaches a growing oarian follicle via blood stream it contributes to the egg yolk formation. Since estrogen is known to have an effect upon male species to induce unusual expression of Vg, an ELISA screening method detecting Vg in EDC exposed male animals is the most reliable bio-screening assay system.

Principle

This kit is based on the ELISA method which uses two different specific anti-Japanese Quail Vg antibodies. Anti-Japanese Quail monoclonal antibody is coated on the microplate, so that Vg in the sample and standard solution is captured. Reacting with anti-Japanese Quail polyclonal antibody and HRP-conjugated anti-Rabbit IgG, the complex of coated-antibody-antigen-antibody is formed. Finally, Vg concentrations are estimated according to the coloring intensity developed with enzymatic reaction.

Contents

(1)Antibody-coated microtiter plate (8 wells×12) : 1 plate
(2)The standard, Japanese Quail Vg, lyophilized : 300µl × 2
(3)Dilution solution : 30mL × 2
(4)Anti-Japanese Quail Lipovitellin polyclonal antibody (×100) : 80µl × 1
(5)HRP-conjugated anti-Rabbit IgG (×100) : 80µl × 1
(6)OPD (o-phenylendiamine) tablets : 2 tab
(7)Substrate buffer : 15 mL × 1
(8)Stop solution : 6 mL × 1
(9)Wash solution (×20) : 30 mL × 1

	Product Name	Cat#	Quantity	Price
	Vitellogenin ELISA	KAL-KH049	1 KIT	¥75,000 $790 €577
	Vitellogenin, ELISA	KAL-KH075	1 KIT	¥80,000 $843 €616
	Vitellogenin ELISA	KAL-KH003	1 KIT	¥90,000 $948 €693

	Product Name	Cat#	Quantity	Price
	Anti Vitellogenin : ZV2-1	EBT-ZV2-1-EX	1 ML	¥50,000 $527 €385
	Anti Vitellogenin	EBT-ZV21-HRP-EX	0.5 ML	¥50,000 $527 €385
	Anti Vitellogenin : Z42-7	EBT-Z42-7-EX	1 ML	¥50,000 $527 €385
	Anti Vitellogenin	EBT-MVD33-HRP-EX	0.5 ML	¥50,000 $527 €385
	Anti Vitellogenin : F61-2	EBT-MUMF61-2-EX	1 ML	¥50,000 $527 €385
	Anti Vitellogenin : B6-1	EBT-MUMB6-1-EX	1 ML	¥50,000 $527 €385
	Anti Vitellogenin : D3-3	EBT-AVMD3-3-EX	1 ML	¥50,000 $527 €385
	Anti Vitellogenin : F10-2	EBT-AVMF10-2-EX	1 ML	¥50,000 $527 €385
	Zebrafish Vitellogenin standard for ELISA	EBT-ZV-STD-EX	1 SET	¥10,000 $106 €77
	Medaka Vitellogenin standard for ELISA	EBT-MV-STD-EX	1 SET	¥10,000 $106 €77
	Carp Vitellogenin Standard for ELISA	EBT-CV-STD-EX	1 SET	¥10,000 $106 €77
	Fathead minnow Vitellogenin Standard for ELISA	EBT-FV-STD-EX	1 SET	¥10,000 $106 €77