 |
|
|
|
For Oxidative Stress Research |
| |
| |
Potential Antioxidant(PAO) assay Kit |
Oxidative stress plays on important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging.Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. Recently, antioxidants as functional foods which scavenge ROS attract a great deal of attention.
In the PAO assay kit, an easy and convenient method to measure antioxidant capacity is provided. Utilizing the reduction of cupric ion (Cu++?Cu+), antioxidant capacity of samples can be detected in 5 minutes. Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine) , and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed. PAO can detect not only hydrophilic antioxidants such as Vitamin C, glutathione, but also can detect hydrophobic antioxidants such as Vitamin E. Applicable for assessment of total antioxidants of serum, foods and beverage samples.
  |
Features
| Assay principle: |
Colormetric assay(detection: 480 - 492 nm) |
| Assay range: |
21.9 - 4378 micro mol/L (cupric ion reducing power) |
| Format: |
96 wells |
| Storage: |
Room temperature (10 - 25°C) |
| Applications: |
Human and animal serum samples, foods and beverage samples. |
| Required but not provided: |
A micro plate reader (measuring wavelength 492 nm)
Pipettes and pipette chips
Plastic test tubes
Distilled water
NaOH, HCl solution and pH meter (Not required if standards are
prepared with distilled water only). |
| Source |
Mouse |
| Immunogen |
4-HNE-modified Keyhole Limpet Hemocyanin |
| Subclass |
IgG1(kappa) |
| Clone |
HNEJ-2 |
| Form |
Lyophilized Powder |
| Specificity |
This antibody show almost negligible reactivity with proteins
that were treated with other aldehydes, such as 2-nonenal, 2-hexenal,
1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde. |
| Applications |
Immunohistochemistry and Western blotting. |
| Storage |
Less than -20°C for 5 years |
Content
| Standard (Uric acid powder): |
1 vial |
| Sample diluent: |
1 bottles |
| Cu++ solution: |
1 bottle |
| Stop solution: |
1 bottle |
| Micro titer plate: |
1 plate (96 wells) |
Assay procedure
| 1) |
Prepare 6 levels of standards by diluting 2mM uric acid. |
| 2) |
Please prepare plastic test tubes for 6 levels of standards
and each sample. Pour 390 micro L of Sample Diluent, and add
10 micro L of standards or diluted samples. |
| 3) |
Pour 200micro L of mixture to Micro titer plate. Use 200 microL
of Sample Diluent for blank well. |
| 4) |
Read absorbance at 490 nm (as READ1). |
| 5) |
Add 50 micro L of Cu++solution to each well, mix gently, and
incubate at room temperature for 3 minutes. |
| 6) |
Add 50 micro L of Stop solution, mix gently, and read absorbance
at 490 nm (as READ2). |
| 7) |
Please draw standard curves by plotting the difference of
absorbance readings (READ2 - READ1) as vertical axis, and concentration
of uric acid standards (mM) as horizontal axis. Calculate the
corresponding uric acid concentration of samples. Multiply corresponding
uric acid concentration (mM) of samples by 2189, to estimate
antioxidant power (micro mol/L).
1mM of uric acid = 2189 micro mol/L (copper reducing power) |
<Reference>
| 1) |
Effetcts of the daily administration of a rehydrating supplement
to trotter horses
A Falaschini, G Marangoni, S Rizzi and MF Trombetta
J Equine Sci 16(1),p1-9 (2005) |
| 2) |
Oxidative imbalance and cathepsin D changes as peripheral
blood biomarkers of Alzheimer disease: A pilot study.
E Strafacea, P Matarresea, L Gambardella, R Vona, A Sgadari,MC
Silveri, W Malorni
FEBS Letters 579, p2759-766 (2005) |
| 3) |
Oxidative stress and its association with coronary artery
disease and different atherogenic risk factors.
C. VASSALLE, L. PETROZZI , N. BOTTO, M. G. ANDREASSI and G.
C. ZUCCHELLI
Journal of Internal Medicine 256: 308-315 (2004) |
| 4) |
Antioxidant capacity as a reliable marker of stress in dairy
calves transported by road.
P Pregel, E Bollo, FT Cannizzo, B Biolatti, E Contato, and PG
Biolatti
Veterinary Record 156, p53-54 (2005) |
| 5) |
Vitamin E-coated dialyzers reduce oxidative stress related
proteins and markers in hemodialysis ? a molecular biological
approach.
LA Calo, A Naso, E Pagnin, PA Davis, M Castoro, R Corradin,
P Riegler, C Cascone, W Huber and A Piccoli
Clinical Nephrology, Vol.62(5), p355-361 (2004) |
| 6) |
Oxidative stress-related factors in Bartter's and Gitelman9s
syndrome: relevance for angiotensin II signalling.
Calo LA, Pagnin E, Davis PA, Sartori M, Semplicini A.
Nephrol Dial Transplant ,Vol.18(8) p1518-25 (2003) |
| 7) |
Effect of epoetin on HO-1 mRNA level and plasma antioxidants
in hemodialysis patients.
Calo LA, Stanic L, Davis PA, Pagnin E, Munaretto G, Fusaro M,
Landini S, Semplicini A, Piccoli A.
Int. J Clin. Ther, Vol.41(5), p187-92 (2003) |
| 8) |
Restored Antioxidant Capacity Parallels the Immunologic and
Virologic Improvement in Children with Perinatal Human Immunodeficiency
Virus Infection Receiving Highly Active Antiretroviral Therapy.
M Martino, F Chiarelli, M Moriondo, M Torello, C Azzari, and
L Galli
Clinical Immunology, Vol.100(1),p82-6 (2001) |
Oxidative stress marker product
| Oxidized DNA damage markers |
| Oxidized Protein damage markers |
|
|
View
cart
New Products from Cosmo Bio
