Can Get Signal® Immunoreaction Enhancer Solution

1: High signal and low background

• This solution greatly enhances antigen-antibody reactions, yielding signals several ten times higher than obtained with conventional methods.
• Two kinds of solution is optimized for primary and secondary antibodies, which can suppress the background and increase the S/N ratio.

2: Wide versatility

• It is applicable to various assay system involving antigen-antibody reactions such as Western blotting, dot blotting and ELISA.
• It is compatible with all of the detection system involving chemical coloration, chemiluminescence and fluorescence. The composition has no affect on the activity of labeled antibodies such as peroxidase and alkaline phosphatase.

3: Ready to use

• It is supplied at a ready-for-use concentration.
• It can be directly used as an antibody diluent instead of the PBS or TBS diluents used with conventional methods.

Detection of phosphorylated protein by Western blotting

Fig 1 shows the result for detecting phosphorylated Akt. Detection of phosphorylated Akt increased as the insulin concentration increased. There was a clear signal enhancement and background suppression in the assay using Can Get Signal compared with the conventional method using TBS-T. This result suggest the use of Can Get Signal yield higher signals and lower backgrounds in Western blotting with antibodies against phosphprylated protein compared with conventional assay methods using TBS-T as a diluent.

(* This data was provided by Dr. Toshihiko Yanagita of Pharmacology, Faculty of Medicine, University of Miyazaki)