PURESYSTEM originally developed by a group of Prof. T. Ueda of the University of Tokyo is a proprietary protein synthesis and purification technology of Post Genome Institute Co., Ltd (PGI). PURESYSTEM is a novel reconstituted cell-free protein synthesis system which consists of purified components necessary for transcription, translation and energy regeneration. In PURESYSTEM, all protein factors for transcription and translation are tagged with hexahistidine: 3 initiation factors (IF1, IF2, IF3), 3 elongation factors (EF-G, EF-Tu, EF-Ts), 3 release factors (RF1, RF2, RF3), ribosome recycling factor, 20 aminoacyl-tRNA synthetases, methionyl-tRNA transformylase and T7 RNA polymerase. The reagents also contain E. coli ribosome, amino acids, NTPs, E. coli tRNA and the energy regeneration system (Figure). More
