 Reconstituted Cell-free Protein Synthesis System@<PURESYSTEM®> Flyer PDF |
Kit of in vitro protein synthesis reconstituted with minimum numbers of factors and enzymes necessary for transcription and translation
[ PURESYSTEM classic II ] Basic PURESYSTEM kit
Background
PURESYSTEM is a novel reconstituted in vitro translation system which consists of about 30 purified enzymes necessary for transcription, translation and energy recycling.
In PURESYSTEM, all the factors for transcription and translation are tagged with hexahistidine; 3 release factors (RF1, RF2, RF3), ribosome recycling factor, 20aminoacyl-tRNA synthetases, methionyl-tRNA formyltransferase and T7 RNA polymerase.
The reagents also contains E.coli 70S ribosome, amino acids, NTPs, E.coli tRNA and energy recycling system, so the target protein can be synthesized just by addition of the template DNA to the reaction.
Yield UP
Contamination DOWN
