Dermatan sulfate (DS) is a sulfated glycosaminoglycan composed of repeating disaccharide units of N-acetyl-D-galactosamine (GalNAc) and D-glucuronic acid (GlcUA) or D-iduronic acid (IdoUA). DS is abundant in skin and exists as unbranched polysaccharide chains covalently linked to the protein core of proteoglycans. This product is prepared by the fluorescent labeling of DS derived from porcine skin according to the method of Ogamo et.al.1). Fluoresceinamine molecules are chemically attached to carboxyl groups of the GlcUA or IdoUA of DS. FADS-B1 contains approximately 80 % of disaccharide units with one sulfate groups as 4-O-sulfation of GalNAc. This solution is dissolved in PBS (-) and sterilized by filtration. The endotoxin content is in accordance with the product specifications. The excitation wavelength is 490~500 nm and the emission wavelength is 515~525 nm. The enclosed Certification of Analysis lists actual values for product specifications.
Constitutional formula of Sodium Dermatan Sulfate
Analysis of binding activities with BMP-4 by fluorescence correlation spectroscopy (FCS)
Fluorescence correlation spectroscopy (FCS) is an analytical method of molecular - association or dissociation with one molecular level. The interaction of molecules can be detected as a prolongation of average diffusion time (DT) derived from a fluorescent molecule. The figure shows the effect of recombinant human bone morphogenetic protein-4 (rhBMP-4) on the DT of various fluoresceinamine-labeled GAGs. The result indicates relatively strong interactions between rhBMP-4 and FACS-E1, FACS-P1 or FAHep-N1. (J Cell Physiol, 2008, 217(3):769-777)
