Philippe Gabant, PhD.
Founder, Head of Business Development, Delphi Genetics S.A.

Since the '90s, bacterial poison/antidote genes have been used in tools for molecular biology. Today, the ccdA, ccdB gene loci of the F plasmid are present in vectors for protein expression and DNA cloning. These genes are either used for selection of recombinant molecules (Gateway vectors, TOPO vectors, Zero Background vectors) and for the maintenance of expression vectors in E.coli. Since 2004 these systems are also used in the StabyExpress™ technology allowing to eliminate antibiotics in biopharmaceutical production.

Nowadays most of the scientists use those genes as tools for their daily research works. Most of the time scientists use those genes ignoring their origin and properties. This lack of information could limit the efficiency of their DNA cloning and protein expression experiments. The aim of this scientific presentation is to present the natural origins antidote poison genes, their properties and open a discussion on their current and future applications.

細菌の毒性/解毒遺伝子は、分子生物学研究のツールとしてよく用いられています。これらの遺伝子の起源や特性を深く知ることで、DNAクローニングやタンパク質発現実験の効率を上げることができます。本セミナーでは、それら遺伝子の背景や特性をご説明するとともに、抗生物質を使わないユニークなタンパク質発現システムStabyExpress™ などをご紹介させていただきます。