記事ID ： 3839

陽イオン性脂質試薬と比べて細胞毒性を抑え、効率の高い遺伝子導入を実現 siRNA トランスフェクション試薬

メーカー名：Santa Cruz Biotechnology, Inc.

Santa Cruz社のsiRNA トランスフェクション試薬は、陽イオン性脂質試薬と比べて細胞毒性を抑え、効率の高いトランスフェクションを実現します。

使用目的

siRNA トランスフェクション試薬のトランスフェクション効率
トランスフェクション効率

**様々なセルラインにおけるChk1 siRNAのトランスフェクション効率**
セルライン	種	細胞タイプ	トランスフェクション効率
A-431 (monolayer)	Human	epidermoid carcinoma	92.63%
ALL-SIL (suspension)	Human	T cell leukemia	97.52%
COLO 320DM (monolayer)	Human	colorectal adenocarcinoma	75.43%
HeLa (monolayer)	Human	epithelioid carcinoma	88.31%
Hep G2 (monolayer)	Human	hepatoblastoma	59.39%
HL-60 (suspension)	Human	acute promyelocytic leukemia	99.75%
Hs68 (monolayer)	Human	foreskin fibroblast	96.98%
Jurkat (suspension)	Human	acute T cell leukemia	99.76%
K-562 (suspension)	Human	chronic myelogenous leukemia	66.97%
Raji (suspension)	Human	B lymphocyte, Burkitt's lym	98.21%
SK-N-MC (monolayer)	Human	neuroepithelioma	98.80%
SW480 (monolayer)	Human	colorectal adenocarcinoma	41.30%
THP-1 (suspension)	Human	monocytic leukemia	50.92%
TT (monolayer)	Human	thyroid, medulla carcinoma	59.79%
WI 38 (monolayer)	Human	normal lung fibroblast	92.87%
C2C12 (monolayer)	Mouse	Muscle myoblast, fibroblast	92.13%
I-11.15 (monolayer)	Mouse	spleen macrophage	96.21%
LADMAC (suspension)	Mouse	bone marrow	99.39%
NIH/3T3 (monolayer)	Mouse	normal embryo fibroblast	63.48%
P19 (monolayer)	Mouse	embryonic carcinoma	48.08%
RAW 264.7 (monolayer)	Mouse	Abelson transformed macrophages	59.76%
Sol 8 (monolayer)	Mouse	soleus muscle myoblast	33.93%
TK-1 (suspension)	Mouse	T lymphocyte lymphoma	99.82%
WEHI-13VAR/L929 (monolayer)	Mouse	fibrosarcoma	67.83%
Mv 1 Lu (monolayer)	Mink	lung	60.09%