培養時間・コストを削減 HeLa細胞抽出物

HeLa細胞は，1952年に樹立されたヒト由来の最初の細胞株で、in vitroでの細胞を用いる試験や研究に幅広く用いられています。一般的にウエスタンブロットのコントロールとして広く使用されており、細胞培養から細胞抽出物の調製までを行う研究者も多数おられます。

しかしHeLa細胞は、継続培養による形質の変化、増殖力が極めて高いことによるラボ内の他細胞へのコンタミネーションのリスク等、その管理に時間や手間を要します。

CILBIOTECH（シルバイオテック）社では、自社開発した完全に自動化された独自の培養技術により、安定した品質のHeLa細胞抽出物を安価に提供しております。HeLa細胞はGLP（優良試験所基準）に準拠した設備で、5×10⁶cells/5（細胞生存率93%〜97%）の一定濃度で培養されています。CILBIOTECH社の製品をご使用頂く事で、培養にかかる時間・コストの削減が可能となります。是非お試し下さい。

1. Barabino, S.M.L., Sproat, B.S. and Lamond, A.I. (1992). Antisense probes targeted to an internal domain in U2 snRNP specifically inhibit the second step of pre-mRNA splicing. Nucl. Ac. Res. 20, 4457-4464.

2. Damier, L., Domenjoud, L. and Branlant, C. (1997). The D1-A2 and D2-A2 pairs of splice sites from human immunodeficiency virus Type 1 are highly efficient in vitro, in spite of an unusual branch site. Biochem. Biophys. Res. Comm. 237, 182-187.

3. Hackel, W.., Fischer, V. and Lührmann, R. (1994). A 69 kD protein that associates reversibly with the Sm core domain of several spliceosomal SnRNP species. J. Cell Biol. 124, 261-272.

4. Kleren, M., Blank, V., Logest, F., Vandekerckhove, J., Lottspeich, F., Le Ball, O., Urban, M.B., Kourilsky, Ph., Bauerle, P.A. and Israel, A; (1990). The DNA inding subunit of NF-KB is identical to factor KBF, and homologous to the rel oncogene product. Cell 62, 1007-1018.

5. Lamm, G.M., Blencowe, B.J., Sproat, B.S., Iribarren, A.M., Ryder, U. and Lamond, A.I. (1991). Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts. Nucl. Ac. Res. 19, 3193-3198.

6. Lauber, J., Plessel, G., Prehn, S., Will, C.L., Fabrizio, P., Grôning, K., Lane, W.S. and Lührmann, R. (1997). The human U4/U6 SnRNP contains 60 and 90 kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p. RNA. 3, 926-941.

7. Plessel, G., Fischer, U. and Lührmann, R, (1994). m3G cap hypermethylation of U1 small nuclear ribonucleoprotein (SnRNP) in vitro : evidence that the U1 small nuclear RNA-(guanosine-N2)methyltransferase is a non-SnRNP cytoplasmic protein that requires a binding site on the Sm core domain. Molecular and Cellular Biology 14, 4160-4172.

8. Ryder, U., Sproat, B.S. and Lamond, A.I. (1990). Sequence-specific affinity selection of mammalian splicing complexes. Nucl. Ac. Res. 18, 7373-7379.

9. Will, C.L., Rümpler, S., Gunnewiek, J.K., Van Venrooij, W. and Lührmann, R. (1996). In vitro reconstitution of mammalian U1 SnRNPs active in splicing : the U1:C protein enhances the formation of early (E) spliceosomal complexes. Nucl. Ac. Res. 24, 4614-4623.

10. Brehm, A., Miska, E.A., MacCance, D.J., Reid, J.L., Bannister, A.J., Kouzarides, T. (1998). Retinoblastoma protein recruits histone deacetylase to repress transcription. Nature 391, 591-601.
11. Cairns, C.A. and White, R.J. (1998) p53 is a general represor of RNA polymerase III transcription. EMBO Journal 17, 3112-3123.

12. Izzard, R.A., Jackson, S.P. and Smith, G.C.M. (1999). Competitive and noncompetitive inhibition of the DNA-dependent protein kinase. Cancer Res. 59, 2581- 2586.

13. Smith, G.C., Cary, R.B., Lakin, N.D., Hann, B.C., Teo, S._H., Chen, D.J. and Jackson, S.P. (1999) Purification and DNA binding properties of the ataxiatelangiectasia gene product ATM. Proc. Natl. Acad. Sci. USA 96, 11134-11139.

14. Hughes, P., Tratner, I., Ducoux, M., Piard, K., Baldacci, G. (1999) Isolation and identification of the third subunit of mammalian DNA polymerase d by PCNAaffinity chromatography of Mouse FM3A cell extracts. Nucleic Acids Res. 27, 2108- 2114.

15. Pethe, K., Aumercier, M., Fort, E., Gatot, C. and Menozzi, F.D. (2000) Characterization of the heparin binding site of the mycobacterial Heparin-binding hemagglutinin adhesin. J.Biol.Chem 275,1.

16. Deplus, R., Brenner, C., Burgers, W.A., Putmans, P., Kouzarides, T., de Launoit, Y., Fuks, F. (2002) Dnmt3L is a transcriptional repressor that recruits histone deacetylase. Nucleic Acid Res. 30, n°17, 3831-3838.

17. Brenner, C., Deplus, R., Didelot, C., Loriot, A., Viré, E., De Smet, C., Gutierrez, A., Danovi, D., Bernard, D., Boon, T., Pelicci, P.G., Amati, B., Kouzarides, T., de Launoit, Y., Di Croce, L. and Fuks, F. (2005) Myc represses transcription through recruitment of DNA methyltransferase corepressor. EMBO Journal 24, 336-346.

18. Burgers, W.A., Blanchon, L., Pradhan, S., de Launoit, Y., Kouzarides, T., Fuks, F. (2007) Viral oncoproteins target the DNA methyltransferases. Oncogene 26, 1650-1655

19. Viré, E., Brenner, C., Deplus, R., Blanchon, L., Fraga, M., Didelot, C., Morey, L., Van Eynde, A., Bernard, D., Vanderwinden, J-M., Bollen, M., Esteller, M., Di Croce, L., de Launoit, Y., and Fuks, F. (2006) The Polycomb group protein EZH2 directly controls DNA methylation. Nature 439, 871-874.

20. Jacquenet, S., Méreau, A., Bilodeau, P.S., Damier, L., Martin Stoltzfus, C. and Branlant, C. (2001) A Second Exon Splicing Silencer within Human Immunodeficiency Virus Type 1 tat Exon 2 Represses Splicing of Tat mRNA and Binds Protein hnRNP H*. J. Biol. Chem., 276, No 44, Issue of November 2, 40464-40475

21. Hoffmann,M.H., Tuncel,J., Shriner,K., Tohidast-Akrad, M.Türk;B., Pinol-Roma,S., Serre, G., Schett,G., Smolen,J ;S., Holmdahl,R. and Steiner,G. (2007) The Rheumatoid Arthritis- Associated Autoantigen hnRNP-A2 (RA33) is a Major Stimulation of Autoimmunity in Rats with Pristane-induced Arthritis. The Journal of Immunology 179, 7568-7576

22. Ropers, D., Ayadi, L., Gattoni, R., Jacquenet, S., Damier, L., Branlant, C. and Stévenin, J. (2004) Differential effects of the SR proteins 9G8, SC35, ASF/SF2 and SRp40 on the utilization of the A1 to A5 splicing sites of HIV-1 RNA. J. Biol. Chem., 279, 29963-29973

23. Gehring, N.H., Lamprinaki, S., Kulozik, A.E. and Hentze, M. (2009) Dissassembly of exon junction complexes by PYM. Cell 137, 536-548 55.

24. Agranat, L., Sperling, J. and Sperling, R. (2010) A novel tissue-specific alternatively spliced form of the A-to-I RNA editing enzyme ADAR2. RNA Biology 7, 1-10

25. Bala, K. (2010) Healing the Achilles Heel of Proteomics. Genetic Engng & Biotechnol. News, Vol.30, N°3, February 1st 58. Spagnolo, L., Rivera-Calzada, A., Pearl, L.H. and Llorca, O. (2006) Threedimensional structure of the human DNAPKcs/Ku70/Ku80 complex assembled on DNA and its implications for DNA DSB repair. Molecular Cell 22 (4) 511-519

26. Rivera-Calzada, A., Spagnolo, L., Pearl, L.H. and Llorca, O. (2007) Structural model of full-length human Ku70-Ku80 heterodimer and its recognition of DNA and DNAPKcs. EMBO Reports 8, 56-62

27. Renz, A. and Fackelmayer, F.O. (1996) Purification and Molecular cloning of the Scaffold Attachment Factor B (SAF-B), a novel human nuclear protein that specifically binds to S/MAR-DNA. Nucleic Acids Res. 24 (5) 843-849

28. Ajuh, P., Sleeman, J., Chusainow, J. and Lamond, A.I. (2001) A direct interaction between the carboxyl-terminal region of CDC 5L and the WD40 domain of PRLG1 is essential for pre-mRNA splicing. J.Biol.Chem 276 (45) 42370-42381

29. Taylor, E.M., Copsey, A.C., Hudson, J.J.R., Vidot, S. and Lehmann, A.R. (2008) Identification of the proteins, including MAGE-G1, that make up the human SMC5-6 protein complex. Molecular and Cellular Biology 28 (4) 1197-1206

30. Taylor, E.M., Copsey, A.C., Hudson, J.J.R., Vidot, S. and Lehmann, A.R. (2008) Identification of the proteins, including MAGE-G1, that make up the human SMC5-6 protein complex. Molecular and Cellular Biology 28 (4) 1197-1206

※  表示価格について