記事ID : 18353
研究用

膜タンパク質の単離、精製、構造研究、機能解析に有用ナノディスク

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多くの膜タンパク質は、界面活性剤を含むバッファーを使用することでその安定性と活性を維持することができます。しかし、界面活性剤に感受性な膜タンパク質も存在し、これらには界面活性剤を含むバッファーは適さず、精製できない(精度や収量が低い)、安定性が低い(崩壊しやすい、または沈殿が激しい)、あるいは活性がない(おそらく膜タンパク質が界面活性剤に感受性であるか、その活性にはリン脂質二重層構造が必要)といった複数の問題があります。
この種の膜タンパク質には、ナノディスク技術が有用で、膜タンパク質の単離、精製、構造研究、および機能解析において役に立ちます。ここ稿では、ナノディスクの組成と構造、ナノディスクとリポソームの比較、および膜タンパク質へのナノディスクの適用について記載します。

Cosmo Bio would like to acknowledge and thank the WUHAN HUAMEI BIOTECH Co., Ltd for providing Nanodiscs information presented here.

ナノディスクの組成と構造

ナノディスクの主な成分は合成リン脂質と膜足場タンパク質(MSPs)としても知られる両親媒性らせん状タンパク質です。膜足場タンパク質は血清アポリポタンパク質に付随する一方で、血清アポリポタンパク質は高密度リポタンパク質の主要成分です。2つの膜足場タンパク質は、リボン形状をとってリン脂質を囲んでいます(図1参照)。すなわち、円盤状のリン脂質二重層ナノディスクです。

円盤状HDLの分子ベルトモデルを基にしたナノディスク構造
図.1 円盤状HDLの分子ベルトモデルを基にしたナノディスク構造の (a) 二重層に対して垂直、(b) 二重層平面からみたモデル図
膜足場タンパク質の2つの単量体(青とシアン)はリン脂質二重層(白色)-直径10nm- の区域周辺に両親媒性螺旋ベルトを形成する。

* The model is courtesy of S. C. Harvey[1].

モデル膜

◆ リポソーム

リポソームは、両親媒性の脂質二重層小胞です。リポソームの調製方法は多数ありますが、最もよく知られている簡単な方法は、Bangham法[2]です。標的脂質を揮発性の有機溶剤に溶解し、不活性ガスで乾燥させます。得られた薄層脂質を水溶性緩衝液とともにボルテックスした後、最後に、超音波処理、均質化、およびサイズ既知のフィルター(通常孔径100〜200nm)を通して放出する、などといった様々な手法で単層気泡へと変換します。ただし、これによって不安定性、不均質性、および再現性の低さといったリポソームの明らな欠点が生じます。
リポソームは簡単に凝集や融合を生じ、長期におよぶ操作や流れを止める、激しく混合する、といった特定の物理的処理において不安定になることがあります。押し出し機で成形したリポソームの大きさは均質ではなく、ロットごとの差が大きくなることもあります[3]。リポソーム内に会合された膜タンパク質は、しばしば濁った粘稠性のものになり、特定の生物学的分析技術が使用できない可能性もあります。

◆ ナノディスク

ナノディスクの調製には、リン脂質、膜足場タンパク質、および界面活性剤を含むマスターミックスの調製があり、界面活性剤除去後、残りの成分は直径8〜16nmのナノディスクとよばれる円盤状リン脂質二重層として会合します[4]。ナノディスクの直径はMSPバンドの長さで決まり、リン脂質がMSPと最適なモル比率にある場合は均質なナノディスクが形成されます。
ナノディスクは、リポソームに比べてより均質(1回の調製ごとに)かつ一貫性(異なる調製バッチにおいて)があります。正確な粒子サイズであること、MSP配列長を調整して大きさを変えられること、そして安定であることがリポソームと比較した場合のナノディスクの利点です。

膜タンパク質におけるナノディスクのアプリケーション

ナノディスク技術は、構造研究をはじめ、構造生物学、電子顕微鏡、NMR、EPR、および分光測定検出といった様々な技術分野に適用することができます。

特色とアプリケーション 実験手法 膜タンパク質
可溶化と安定化 X線結晶解析
NMR
共鳴ラマンスペクトル法
光学吸収
EPR
無細胞発現
GPCR
さまざまな膜タンパク質
チトクロムP450
チトクロムP450とロドプシン
トランスポータとロドプシン
多様
足場タンパク質や脂質上の親和性タグを用いた表面固定化 単分子研究、SPR、LSPRおよび感知と検出 チトクロムP450、GPCR、および血液凝固第VII因子
単量体単離、多量体、および複数タンパク質の機能的複合体 構造および機能研究 受容体とロドプシン
CPRとATPaseを含むチトクロムP450
構造的に均質な調製 SAXSとSANS
EM
チトクロムP450と還元酵素
受容体とチャネル
大きさ、電荷、または密度による分画 クロマトグラフィ、電気泳動、および超遠心 多様
タンパク質-膜間結合 SPR K-Rasタンパク質、TF-VIIaおよびPgPトランスポータ
タンパク質の性質への膜効果 酸化還元電位
動態学
チトクロムP450還元酵素
血液凝固第VIII因子
呼吸器複合体

表.1 ナノディスクに抱合された膜タンパク質に使用された手法やツール
SPR: 表面プラズモン共鳴; LSPR: 局在化SPR; CPR: NADPH-チトクロムP450還元酵素[5].

In Vitro E.coliタンパク質発現とナノディスク技術を用いたアポクリン発現

WUHAN HUAMEI BIOTECH社では、in vitro E.coli タンパク質発現とナノディスク技術を組み合わせ、あらかじめ合させたナノディスクをin vitro E.coli タンパク質発現システムに取り入れました。膜タンパク質の翻訳ステップでにこれをナノディスクに会合させ、膜タンパク質 - ナノディスク複合体を形成させました(図2)。この疑似的人工脂質環境は、膜タンパク質に対するさまざまな脂質の影響を分析する新規の手段となり、膜タンパク質の研究に役立ちます。

MSPナノディスクの模式図
図.2 7つの膜貫通タンパク質が包埋されたMSPナノディスクの模式図
直径:およそ10.6 nm。

* Picture from Sligar.

SDS-PAGE結果
図.3 ナノディスクとE.coli アクアポリンZ(aqpZ)を良好に会合した後のSDS-PAGE結果
図2に示したように、MSPと標的タンパク質である膜タンパク質aqpZの2つのバンドが確認できる。

参考文献

  1. Applications of Phospholipid Bilayer Nanodiscs in the Study of Membranes and Membrane Proteins. Abhinav Nath, William M. Atkins, and Stephen G. Sligar. Biochemistry, 2007, 46 (8), 2059-2069
  2. Large volume liposomes by an ether vaporization method. D Deamer, AD Bangham. Biochimica et Biophysica Acta, 443 (1976) 629-~34
  3. Applications of Phospholipid Bilayer Nanodiscs in the Study of Membranes and Membrane Proteins. Abhinav Nath, William M. Atkins, and Stephen G. Sligar. Biochemistry, 2007, 46 (8), 2059-2069
  4. Self-assembly of discoidal phospholipid bilayer nanoparticles with membrane scaffold proteins. Timothy H. Bayburt,Yelena V. Grinkova, and Stephen G. Sligar. Nano Letters, 2002, 2 (8), pp 853-856
  5. Nanodiscs for structural and functional studies of membrane proteins.Ilia G Denisov & Stephen G Sligar. Nature Structural & Molecular Biology 23, 481-486 (2016)

商品リスト

アクアポリン関連 リコンビナント膜貫通タンパク質

Uniprot ID 品番 Name Gene names Length
Q9NHW7 CSB-CF868261AXQ Recombinant Aedes aegypti Aquaporin AQPAe.a AAEL003512 1-249
Q704E8 CSB-CF761659RA Recombinant Rat ATP-binding cassette sub-family B member 7, mitochondrial Abcb7 1-752
Q9QY44 CSB-CF874120RA Recombinant Rat ATP-binding cassette sub-family D member 2 Abcd2 1-741
Q7PWV1 CSB-CF755893BZL Recombinant Anopheles gambiae Aquaporin AQPAn.G AGAP008842 1-250
Q1M1A0 CSB-CF629818EKI Recombinant Encephalitozoon hellem Aquaporin AQP 1-251
Q8SRK2 CSB-CF844644EKH Recombinant Encephalitozoon cuniculi Aquaporin AQP 1-250
Q1M2P4 CSB-CF631887EIX Recombinant Encephalitozoon intestinalis Aquaporin AQP 1-251
B7XIC4 CSB-CF490233EMA Recombinant Enterocytozoon bieneusi Aquaporin AQP 1-242
Q23808 CSB-CF631526CBAU Recombinant Cicadella viridis Aquaporin AQPcic AQP 1-255
C4VBN2 CSB-CF505319NHO Recombinant Nosema ceranae Aquaporin AQP 1-249
Q02013 CSB-CF586908MO Recombinant Mouse Aquaporin-1 Aqp1 1-269
P29975 CSB-CF001957RA Recombinant Rat Aquaporin-1 Aqp1 1-269
P47865 CSB-CF001957BO Recombinant Bovine Aquaporin-1 AQP1 1-271
Q9N2J4 CSB-CF889026DO Recombinant Dog Aquaporin-1 AQP1 1-271
P56401 CSB-CF001957SH Recombinant Sheep Aquaporin-1 AQP1 1-272
Q6PQZ1 CSB-CF744142PI Recombinant Pig Aquaporin-1 AQP1 1-271
Q5R819 CSB-CF722331PYX Recombinant Pongo abelii Aquaporin-1 AQP1 1-269
G5CTF8 CSB-CF974287ZBP Recombinant Milnesium tardigradum Aquaporin-1 AQP1 1-333
Q9FY14 CSB-CF861897MQP Recombinant Medicago truncatula Probable aquaporin TIP-type AQP1 1-250
P29972 CSB-CF001957HU(A4) Recombinant Human Aquaporin-1 AQP1 2-269
G5CTG7 CSB-CF949719ZBP Recombinant Milnesium tardigradum Aquaporin-10 AQP10 1-409
Q96PS8 CSB-CF853482HU Recombinant Human Aquaporin-10 AQP10 1-301
Q09369 CSB-CF600621CXY Recombinant Caenorhabditis elegansPutative aquaporin-10 aqp-10 1-280
G5CTG8 CSB-CF272240ZBP Recombinant Milnesium tardigradum Aquaporin-11 AQP11 1-270
Q8BHH1 CSB-CF804799MO Recombinant Mouse Aquaporin-11 Aqp11 1-271
Q8NBQ7 CSB-CF847678HU Recombinant Human Aquaporin-11 AQP11 1-271
Q8CHM1 CSB-CF213321RA Recombinant Rat Aquaporin-11 Aqp11 1-271
Q8CHJ2 CSB-CF807515MO Recombinant Mouse Aquaporin-12 Aqp12 1-290
Q8IXF9 CSB-CF816900HU Recombinant Human Aquaporin-12A AQP12A 1-295
A6NM10 CSB-CF001961HU Recombinant Human Aquaporin-12B AQP12B 1-295
P41181 CSB-CF001962HU Recombinant Human Aquaporin-2 AQP2 1-271
P34080 CSB-CF001962RA Recombinant Rat Aquaporin-2 Aqp2 1-271
P56402 CSB-CF001962MO Recombinant Mouse Aquaporin-2 Aqp2 1-271
O77697 CSB-CF001962BYB Recombinant Amblysomus hottentotus Aquaporin-2 AQP2 1-109
P79229 CSB-CF001962EYL Recombinant Procavia capensis habessinica Aquaporin-2 AQP2 1-109
P79164 CSB-CF001962DIO Recombinant Dasypus novemcinctus Aquaporin-2 AQP2 1-109
P79168 CSB-CF001962EKA Recombinant Elephas maximus Aquaporin-2 AQP2 1-109
P79165 CSB-CF001962HO Recombinant Horse Aquaporin-2 AQP2 1-109
O62735 CSB-CF001962SH Recombinant Sheep Aquaporin-2 AQP2 1-271
P79099 CSB-CF001962BO Recombinant Bovine Aquaporin-2 AQP2 1-271
O77714 CSB-CF001962DMV Recombinant Dugong dugon Aquaporin-2 AQP2 1-109
O77722 CSB-CF001962EMQ Recombinant Erinaceus europaeus Aquaporin-2 AQP2 1-109
P79803 CSB-CF001962MPI Recombinant Macroscelides proboscideus Aquaporin-2 AQP2 1-111
P79200 CSB-CF001962OFC Recombinant Orycteropus afer Aquaporin-2 AQP2 1-109
P79213 CSB-CF001962RB Recombinant Rabbit Aquaporin-2 AQP2 1-109
P79144 CSB-CF001962DO Recombinant Dog Aquaporin-2 AQP2 1-109
G5CTF9 CSB-CF260636ZBP Recombinant Milnesium tardigradum Aquaporin-2 AQP2 1-323
O77740 CSB-CF001962TLA Recombinant Talpa europaea Aquaporin-2 AQP2 1-109
Q8R2N1 CSB-CF823166MO Recombinant Mouse Aquaporin-3 Aqp3 1-292
P47862 CSB-CF001963RA Recombinant Rat Aquaporin-3 Aqp3 1-292
Q08DE6 CSB-CF958823BO Recombinant Bovine Aquaporin-3 AQP3 1-292
G5CTG0 CSB-CF135860ZBP Recombinant Milnesium tardigradum Aquaporin-3 AQP3 1-326
A9Y006 CSB-CF001963PI Recombinant Pig Aquaporin-3 AQP3 1-290
Q92482 CSB-CF849752HU Recombinant Human Aquaporin-3 AQP3 1-292
P47863 CSB-CF001964RA Recombinant Rat Aquaporin-4 Aqp4 1-323
P55087 CSB-CF001964HU Recombinant Human Aquaporin-4 AQP4 1-323
O77750 CSB-CF001964BO Recombinant Bovine Aquaporin-4 AQP4 1-323
G5CTG1 CSB-CF931276ZBP Recombinant Milnesium tardigradum Aquaporin-4 AQP4 1-349
P55088 CSB-CF001964MO Recombinant Mouse Aquaporin-4 Aqp4 1-323
Q5I4F9 CSB-CF692207NBAK Recombinant Notomys alexis Aquaporin-4 AQP4 1-326
Q923J4 CSB-CF835655DDAO Recombinant Dipodomys merriami Aquaporin-4 AQP4 1-324
P55064 CSB-CF001965HU Recombinant Human Aquaporin-5 AQP5 1-265
Q9WTY4 CSB-CF896235MO Recombinant Mouse Aquaporin-5 Aqp5 1-265
A8W649 CSB-CF001965PI Recombinant Pig Aquaporin-5 AQP5 1-265
Q866S3 CSB-CF774730SH Recombinant Sheep Aquaporin-5 AQP5 1-265
G5CTG2 CSB-CF268256ZBP Recombinant Milnesium tardigradum Aquaporin-5 AQP5 1-277
P47864 CSB-CF001965RA Recombinant Rat Aquaporin-5 Aqp5 1-265
G5CTG3 CSB-CF294052ZBP Recombinant Milnesium tardigradum Aquaporin-6 AQP6 1-285
Q8C4A0 CSB-CF806019MO Recombinant Mouse Aquaporin-6 Aqp6 1-293
Q13520 CSB-CF618784HU Recombinant Human Aquaporin-6 AQP6 1-282
Q9WTY0 CSB-CF894353RA Recombinant Rat Aquaporin-6 Aqp6 1-276
O14520 CSB-CF001967HU Recombinant Human Aquaporin-7 AQP7 1-342
Q4R691 CSB-CF703115MOV Recombinant Macaca fascicularis Aquaporin-7 AQP7 1-342
G5CTG4 CSB-CF103847ZBP Recombinant Milnesium tardigradum Aquaporin-7 AQP7 1-315
O54794 CSB-CF001967MO Recombinant Mouse Aquaporin-7 Aqp7 1-303
P56403 CSB-CF001967RA Recombinant Rat Aquaporin-7 Aqp7 1-269
A6NL99 CSB-CF001970HU Recombinant Human Putative aquaporin-7-like protein 3 AQP7P3 1-342
O94778 CSB-CF001972HU Recombinant Human Aquaporin-8 AQP8 1-261
Q5I4F8 CSB-CF702429NBAK Recombinant Notomys alexis Aquaporin-8 AQP8 1-261
P56405 CSB-CF001972RA Recombinant Rat Aquaporin-8 Aqp8 1-263
G5CTG5 CSB-CF547161ZBP Recombinant Milnesium tardigradum Aquaporin-8 AQP8 1-342
P56404 CSB-CF001972MO Recombinant Mouse Aquaporin-8 Aqp8 1-261
O43315 CSB-CF001973HU Recombinant Human Aquaporin-9 AQP9 1-295
Q9JJJ3 CSB-CF888326MO Recombinant Mouse Aquaporin-9 Aqp9 1-295
G5CTG6 CSB-CF829574ZBP Recombinant Milnesium tardigradum Aquaporin-9 AQP9 1-281
P56627 CSB-CF001973RA Recombinant Rat Aquaporin-9 Aqp9 1-295
Q9U8P7 CSB-CF866191DKK Recombinant Dictyostelium discoideum Aquaporin A aqpA 1-279
P50501 CSB-CF342296RAW Recombinant Pelophylax esculentus Aquaporin FA-CHIP AQPA 1-272
Q54WT8 CSB-CF706896DKK Recombinant Dictyostelium discoideum Aquaporin-B aqpB 1-294
O28846 CSB-CF521560DOC Recombinant Archaeoglobus fulgidus Probable aquaporin AqpM aqpM 1-246
O26206 CSB-CF521385MSR Recombinant Methanothermobacter thermautotrophicus Aquaporin AqpM aqpM 1-246
Q9C4Z5 CSB-CF863199MSQ Recombinant Methanothermobacter marburgensis Aquaporin AqpM aqpM 1-246
Q7W917 CSB-CF767021BIK Recombinant Bordetella parapertussis Aquaporin Z aqpZ 1-236
P60845 CSB-CF350280EGX Recombinant Escherichia coli O6:H1 Aquaporin Z aqpZ 1-231
Q88F17 CSB-CF771793FGB Recombinant Pseudomonas putida Aquaporin Z aqpZ 1-230
Q8EHC1 CSB-CF815044SGA Recombinant Shewanella oneidensis Aquaporin Z aqpZ 1-231
P73809 CSB-CF111763SSQ Recombinant Synechocystis sp. Aquaporin Z aqpZ 1-247
Q7WKG2 CSB-CF760296BTY Recombinant Bordetella bronchiseptica Aquaporin Z aqpZ 1-236
Q2YR68 CSB-CF652178BMN Recombinant Brucella abortus Aquaporin Z aqpZ 1-228
Q9L772 CSB-CF873227BMO Recombinant Brucella melitensis biotype 1 Aquaporin Z aqpZ 1-228
Q7NU39 CSB-CF742343CKA Recombinant Chromobacterium violaceum Aquaporin Z aqpZ 1-229
Q7NNP3 CSB-CF742312GCI Recombinant Gloeobacter violaceus Aquaporin Z aqpZ 1-248
Q9HWZ3 CSB-CF867359EZX Recombinant Pseudomonas aeruginosa Aquaporin Z aqpZ 1-229
P60925 CSB-CF351570RLU Recombinant Rhodopseudomonas palustris Aquaporin Z aqpZ 1-240
O68874 CSB-CF524789SZB Recombinant Shigella flexneriAquaporin Z aqpZ 1-231
Q8DB17 CSB-CF816087VFI Recombinant Vibrio vulnificus Aquaporin Z aqpZ 1-231
Q89EG9 CSB-CF805106BVW Recombinant Bradyrhizobium diazoefficiens Aquaporin Z aqpZ 1-240
P0C112 CSB-CF314232BWS Recombinant Brucella abortus biovar 1 Aquaporin Z aqpZ 1-228
Q8FY85 CSB-CF810062BMQ Recombinant Brucella suis biovar 1 Aquaporin Z aqpZ 1-228
Q8X6K6 CSB-CF834788EOD Recombinant Escherichia coli O157:H7Aquaporin Z aqpZ 1-231
Q7WVD3 CSB-CF773691FAAX Recombinant Flavobacterium johnsoniae Aquaporin Z aqpZ 1-79
Q7N5C1 CSB-CF766216PIJ Recombinant Photorhabdus luminescens subsp. laumondii Aquaporin Z aqpZ 1-231
Q87MQ5 CSB-CF771617VFE Recombinant Vibrio parahaemolyticus serotype O3:K6 Aquaporin Z aqpZ 1-232
Q7MIV9 CSB-CF752212VCQ Recombinant Vibrio vulnificus Aquaporin Z aqpZ 1-231
P60844 CSB-CF352724ENV Recombinant Escherichia coli Aquaporin Z aqpZ 1-231
Q8UI24 CSB-CF819702AYS Recombinant Agrobacterium fabrum Aquaporin Z 1 aqpZ1 1-241
Q92NM3 CSB-CF821843RKU Recombinant Rhizobium meliloti Aquaporin Z 1 aqpZ1 1-228
Q8UJW4 CSB-CF837674AYS Recombinant Agrobacterium fabrum Aquaporin Z 2 aqpZ2 1-228
Q92ZW9 CSB-CF846291RKU Recombinant Rhizobium meliloti Aquaporin Z 2 aqpZ2 1-232
A6ZX66 CSB-CF001957STA Recombinant Saccharomyces cerevisiae Aquaporin-1 AQY1 1-305
C8ZJM1 CSB-CF001957SVN Recombinant Saccharomyces cerevisiae Aquaporin-1 AQY1 1-327
P0CD92 CSB-CF001957SAC Recombinant Saccharomyces cerevisiae Aquaporin-1 AQY1 1-327
P0CD91 CSB-CF001957SVG Recombinant Saccharomyces cerevisiae Aquaporin-1 AQY1 1-305
P0CD90 CSB-CF315989SVG Recombinant Saccharomyces cerevisiae Aquaporin-like protein 2 AQY2 1-149
P0CD89 CSB-CF001962SAC Recombinant Saccharomyces cerevisiae Aquaporin-2 AQY2 1-289
C7GNE3 CSB-CF513127SVL Recombinant Saccharomyces cerevisiae Aquaporin-like protein 2 AQY2-2 1-149
C8ZCS3 CSB-CF513909SVN Recombinant Saccharomyces cerevisiae Aquaporin-like protein 2 AQY2-2 1-149
B3LTC5 CSB-CF457277SVP Recombinant Saccharomyces cerevisiae Aquaporin-like protein 2 AQY2-2 1-149
A7A0K8 CSB-CF409777STA Recombinant Saccharomyces cerevisiae Aquaporin-like protein 2 AQY2-2 1-149
P30518 CSB-CF002470HU Recombinant Human Vasopressin V2 receptor AVPR2 1-371
O35789 CSB-CF002496RA Recombinant Rat Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 B3gat1 1-334
P23645 CSB-CF326081DLU Recombinant Drosophila melanogaster Neurogenic protein big brain bib 1-696
C7GNE2 CSB-CF509781SVL Recombinant Saccharomyces cerevisiae Putative uncharacterized protein C1Q_01798 C1Q_01798 1-152
P70500 CSB-CF005059RA Recombinant Rat CDP-diacylglycerol--inositol 3-phosphatidyltransferase Cdipt 1-213
P04757 CSB-CF005389RA Recombinant Rat Neuronal acetylcholine receptor subunit alpha-3 Chrna3 26-499
O43169 CSB-CF006310HU Recombinant Human Cytochrome b5 type B CYB5B 12-146
Q9CQX2 CSB-CF863430MO Recombinant Mouse Cytochrome b5 type B Cyb5b 12-146
Q5RDJ5 CSB-CF732778PYX Recombinant Pongo abelii Cytochrome b5 type B CYB5B 12-146
P04166 CSB-CF006310RA Recombinant Rat Cytochrome b5 type B Cyb5b 12-146
P33560 CSB-CF330266DNE Recombinant Antirrhinum majus Probable aquaporin TIP-type DIP 1-250
Q9V5Z7 CSB-CF893235DLU Recombinant Drosophila melanogaster Aquaporin Drip 1-245
C8ZCS2 CSB-CF513181SVN Recombinant Saccharomyces cerevisiae Putative uncharacterized protein EC1118_1L10_0100g EC1118_1L10_0100g 1-152
Q5M871 CSB-CF007972RA Recombinant Rat Fas apoptotic inhibitory molecule 3 Fcmr 18-426
P35839 CSB-CF330494MET Recombinant Methanobacterium formicicumProbable formate transporter fdhC 1-280
Q50568 CSB-CF681394MEV Recombinant Methanothermobacter thermautotrophicus Probable formate transporter fdhC 1-274
P0AC24 CSB-CF359707EGX Recombinant Escherichia coli O6:H1 Probable formate transporter 1 focA 1-285
P0AC23 CSB-CF364738ENV Recombinant Escherichia coli Probable formate transporter 1 focA 1-285
P0AC25 CSB-CF364739EOD Recombinant Escherichia coli O157:H7Probable formate transporter 1 focA 1-285
P43756 CSB-CF337509HTA Recombinant Haemophilus influenzae Probable formate transporter focA 1-284
P77733 CSB-CF301248ENV Recombinant Escherichia coli Probable formate transporter 2 focB 1-282
P23900 CSB-CF326102SVG Recombinant Saccharomyces cerevisiae Glycerol uptake/efflux facilitator protein FPS1 1-669
P63079 CSB-CF009148RA Recombinant Rat Gamma-aminobutyric acid receptor subunit beta-3 Gabrb3 26-473
P29415 CSB-CF009446CH Recombinant Chicken Gap junction alpha-3 protein GJA3 2-511
P36381 CSB-CF009449CH Recombinant Chicken Gap junction alpha-8 protein GJA8 2-400
Q9CE02 CSB-CF874977LNG Recombinant Lactococcus lactis subsp. lactis Glycerol facilitator-aquaporin gla gla 1-289
P22094 CSB-CF325214LNF Recombinant Lactococcus lactis subsp. cremoris Glycerol facilitator-aquaporin gla gla 1-289
P18156 CSB-CF324691BRJ Recombinant Bacillus subtilis Glycerol uptake facilitator protein glpF 1-274
P0AER1 CSB-CF360150EGX Recombinant Escherichia coli O6:H1 Glycerol uptake facilitator protein glpF 1-281
P0AER0 CSB-CF365183ENV Recombinant Escherichia coli Glycerol uptake facilitator protein glpF 1-281
P0A3Q7 CSB-CF363431FMW Recombinant Streptococcus pneumoniae serotype 4 Glycerol uptake facilitator protein glpF 1-234
P47279 CSB-CF343650MLN Recombinant Mycoplasma genitalium Probable glycerol uptake facilitator protein glpF 1-258
P31140 CSB-CF335607SZB Recombinant Shigella flexneriGlycerol uptake facilitator protein glpF 1-281
Q8K9M9 CSB-CF814361BXE Recombinant Buchnera aphidicola subsp. Schizaphis graminum Glycerol uptake facilitator protein glpF 1-262
P52280 CSB-CF346741MWI Recombinant Mycoplasma gallisepticum Probable glycerol uptake facilitator protein glpF 1-243
P19255 CSB-CF324786FOB Recombinant Streptomyces coelicolor Probable glycerol uptake facilitator protein glpF 1-264
P57392 CSB-CF348981BWZ Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Glycerol uptake facilitator protein glpF 1-263
P0AER2 CSB-CF365184EOD Recombinant Escherichia coli O157:H7Glycerol uptake facilitator protein glpF 1-281
P44826 CSB-CF337954HTA Recombinant Haemophilus influenzae Glycerol uptake facilitator protein glpF 1-264
P75071 CSB-CF301897MLW Recombinant Mycoplasma pneumoniae Probable glycerol uptake facilitator protein glpF 1-264
Q51389 CSB-CF706547EZX Recombinant Pseudomonas aeruginosa Glycerol uptake facilitator protein glpF 1-279
P0A3Q8 CSB-CF358405SMP Recombinant Streptococcus pneumoniae Glycerol uptake facilitator protein glpF 1-234
Q9X1E3 CSB-CF894430TNJ Recombinant Thermotoga maritima Probable glycerol uptake facilitator protein glpF 1-234
F9USY3 CSB-CF212756LMS Recombinant Lactobacillus plantarum Glycerol uptake facilitator protein 2 glpF2 1-235
F9UTW9 CSB-CF194580LMS Recombinant Lactobacillus plantarum Glycerol uptake facilitator protein 3 glpF3 1-240
F9UMX3 CSB-CF978635LMS Recombinant Lactobacillus plantarum Glycerol uptake facilitator protein 4 glpF4 1-238
F9UUB3 CSB-CF279962LMS Recombinant Lactobacillus plantarum Glycerol uptake facilitator protein-like 5 glpF5 1-250
F9UUD2 CSB-CF950889LMS Recombinant Lactobacillus plantarum Glycerol uptake facilitator protein-like 6 glpF6 1-216
O86231 CSB-CF530776HTA Recombinant Haemophilus influenzae Uncharacterized aquaporin-like protein HI_1017 HI_1017 1-213
P31388 CSB-CF010898RA Recombinant Rat 5-hydroxytryptamine receptor 6 Htr6 1-436
P70606 CSB-CF012083RA Recombinant Rat Small conductance calcium-activated potassium channel protein 1 Kcnn1 1-536
P70604 CSB-CF012084RA Recombinant Rat Small conductance calcium-activated potassium channel protein 2 Kcnn2 1-580
F9UST3 CSB-CF167360LMS Recombinant Lactobacillus plantarum D/L-lactic acid transporter larD 1-238
P42067 CSB-CF331409MQO Recombinant Medicago sativa Probable aquaporin TIP-type MCP1 1-249
Q08834 CSB-CF147087RA Recombinant Rat Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A Mgat5 1-740
P30301 CSB-CF013834HU Recombinant Human Lens fiber major intrinsic protein MIP 1-263
P06624 CSB-CF013834BO Recombinant Bovine Lens fiber major intrinsic protein MIP 1-263
Q6RZ07 CSB-CF740854GU Recombinant Guinea pig Lens fiber major intrinsic protein MIP 1-263
P28238 CSB-CF013834CH Recombinant Chicken Lens fiber major intrinsic protein MIP 1-262
P09011 CSB-CF013834RA Recombinant Rat Lens fiber major intrinsic protein Mip 1-261
A2IBY8 CSB-CF013834DO Recombinant Dog Lens fiber major intrinsic protein MIP 1-263
A4L9J0 CSB-CF013834RB Recombinant Rabbit Lens fiber major intrinsic protein MIP 1-263
P51180 CSB-CF013834MO Recombinant Mouse Lens fiber major intrinsic protein Mip 1-263
Q6J8I9 CSB-CF764270SH Recombinant Sheep Lens fiber major intrinsic protein MIP 1-263
Q25074 CSB-CF638811HAAO Recombinant Haematobia irritans exigua Aquaporin N/A 1-251
Q06019 CSB-CF281361RJN Recombinant Lithobates pipiens Lens fiber major intrinsic protein N/A 1-263
P08995 CSB-CF362572GGV Recombinant Glycine max Nodulin-26 N/A 1-271
P49173 CSB-CF341802NDF Recombinant Nicotiana alata Probable aquaporin NIP-type N/A 1-270
Q08451 CSB-CF871510SZY Recombinant Solanum lycopersicum Probable aquaporin PIP-type pTOM75 N/A 1-286
P42767 CSB-CF337203AQS Recombinant Atriplex canescens Aquaporin PIP-type N/A 1-282
P21653 CSB-CF322002NHE Recombinant Nicotiana tabacum Probable aquaporin TIP-type RB7-5A N/A 1-250
P24422 CSB-CF328469NHE Recombinant Nicotiana tabacum Probable aquaporin TIP-type RB7-18C N/A 1-250
P23958 CSB-CF329433EUN Recombinant Phaseolus vulgaris Probable aquaporin TIP-type alpha N/A 1-256
Q9ATN4 CSB-CF858339ZAX Recombinant Zea mays Aquaporin NIP1-1 NIP1-1 1-282
Q40746 CSB-CF669214OFG Recombinant Oryza sativa subsp. japonica Aquaporin NIP1-1 NIP1-1 1-284
Q8VZW1 CSB-CF845080DOA Recombinant Arabidopsis thaliana Aquaporin NIP1-1 NIP1-1 1-296
Q0JPT5 CSB-CF603826OFG Recombinant Oryza sativa subsp. japonica Aquaporin NIP1-2 NIP1-2 1-303
Q8LFP7 CSB-CF853929DOA Recombinant Arabidopsis thaliana Aquaporin NIP1-2 NIP1-2 1-294
Q0DK16 CSB-CF609154OFG Recombinant Oryza sativa subsp. japonica Aquaporin NIP1-3 NIP1-3 1-286
Q5Z9E2 CSB-CF726469OFG Recombinant Oryza sativa subsp. japonica Aquaporin NIP1-4 NIP1-4 1-273
Q19KC1 CSB-CF618320ZAX Recombinant Zea mays Aquaporin NIP2-1 NIP2-1 1-295
Q6Z2T3 CSB-CF744376OFG Recombinant Oryza sativa subsp. japonica Aquaporin NIP2-1 NIP2-1 1-298
Q8W037 CSB-CF851901DOA Recombinant Arabidopsis thaliana Aquaporin NIP2-1 NIP2-1 1-288
Q9ATN2 CSB-CF860169ZAX Recombinant Zea mays Aquaporin NIP2-2 NIP2-2 1-294
Q67WJ8 CSB-CF714994OFG Recombinant Oryza sativa subsp. japonica Aquaporin NIP2-2 NIP2-2 1-298
Q9AT74 CSB-CF861006ZAX Recombinant Zea mays Aquaporin NIP2-3 NIP2-3 1-301
Q9C6T0 CSB-CF883695DOA Recombinant Arabidopsis thaliana Aquaporin NIP3-1 NIP3-1 1-323
Q0IWF3 CSB-CF611192OFG Recombinant Oryza sativa subsp. japonica Aquaporin NIP3-1 NIP3-1 1-311
Q9ATN1 CSB-CF857690ZAX Recombinant Zea mays Aquaporin NIP3-1 NIP3-1 1-302
Q7EYH7 CSB-CF752036OFG Recombinant Oryza sativa subsp. japonica Aquaporin NIP3-2 NIP3-2 1-305
Q84S07 CSB-CF772832OFG Recombinant Oryza sativa subsp. japonica Aquaporin NIP3-3 NIP3-3 1-278
Q9ASI1 CSB-CF858332OFG Recombinant Oryza sativa subsp. japonica Aquaporin NIP4-1 NIP4-1 1-286
Q9FIZ9 CSB-CF872246DOA Recombinant Arabidopsis thaliana Putative aquaporin NIP4-1 NIP4-1 1-283
Q8W036 CSB-CF855405DOA Recombinant Arabidopsis thaliana Probable aquaporin NIP4-2 NIP4-2 1-283
Q9SV84 CSB-CF890454DOA Recombinant Arabidopsis thaliana Probable aquaporin NIP5-1 NIP5-1 1-304
Q9SAI4 CSB-CF871020DOA Recombinant Arabidopsis thaliana Aquaporin NIP6-1 NIP6-1 1-305
Q8LAI1 CSB-CF817165DOA Recombinant Arabidopsis thaliana Probable aquaporin NIP7-1 NIP7-1 1-275
P25926 CSB-CF326253SXB Recombinant Salmonella typhimurium Nitrite transporter NirC nirC 1-269
P0AC27 CSB-CF359709SZB Recombinant Shigella flexneriNitrite transporter NirC nirC 1-268
P0AC26 CSB-CF359708ENV Recombinant Escherichia coli Nitrite transporter NirC nirC 1-268
P43322 CSB-CF016077RA Recombinant Rat Pro-neuregulin-1, membrane-bound isoform Nrg1 14-662
Q9EPX4 CSB-CF872110RA Recombinant Rat P2Y purinoceptor 12 P2ry12 1-343
Q62011 CSB-CF017739MO Recombinant Mouse Podoplanin Pdpn 23-172
Q95152 CSB-CF017739DO Recombinant Dog Podoplanin PDPN 23-169
Q86YL7 CSB-CF017739HU Recombinant Human Podoplanin PDPN 23-162
Q64294 CSB-CF017739RA Recombinant Rat Podoplanin Pdpn 23-166
P37451 CSB-CF334266SXB Recombinant Salmonella typhimurium Propanediol diffusion facilitator pduF 1-264
P70580 CSB-CF017876RA Recombinant Rat Membrane-associated progesterone receptor component 1 Pgrmc1 2-195
P61838 CSB-CF351097VFJ Recombinant Vicia faba Aquaporin PIP1.1 PIP1.1 1-286
Q39196 CSB-CF653696DOA Recombinant Arabidopsis thaliana Probable aquaporin PIP1-4 PIP1.4 1-287
Q41870 CSB-CF671453ZAX Recombinant Zea mays Aquaporin PIP1-1 PIP1-1 1-287
P61837 CSB-CF352256DOA Recombinant Arabidopsis thaliana Aquaporin PIP1-1 PIP1-1 1-286
Q6EU94 CSB-CF738633OFG Recombinant Oryza sativa subsp. japonica Aquaporin PIP1-1 PIP1-1 1-289
Q9XF59 CSB-CF895890ZAX Recombinant Zea mays Aquaporin PIP1-2 PIP1-2 1-289
Q7XSQ9 CSB-CF768697OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin PIP1-2 PIP1-2 1-288
Q06611 CSB-CF445307DOA Recombinant Arabidopsis thaliana Aquaporin PIP1-2 PIP1-2 1-286
Q9SXF8 CSB-CF866014OFG Recombinant Oryza sativa subsp. japonica Aquaporin PIP 1-3 PIP1-3 1-288
Q08733 CSB-CF564049DOA Recombinant Arabidopsis thaliana Aquaporin PIP1-3 PIP1-3 1-286
Q9AQU5 CSB-CF857681ZAX Recombinant Zea mays Aquaporin PIP1-3/PIP1-4 PIP1-3; PIP1-4 1-292
Q9AR14 CSB-CF860164ZAX Recombinant Zea mays Aquaporin PIP1-5 PIP1-5 1-288
Q8LAA6 CSB-CF810555DOA Recombinant Arabidopsis thaliana Probable aquaporin PIP1-5 PIP1-5 1-287
Q9ATN0 CSB-CF861009ZAX Recombinant Zea mays Aquaporin PIP1-6 PIP1-6 1-296
P43286 CSB-CF337370DOA Recombinant Arabidopsis thaliana Aquaporin PIP2-1 PIP2-1 2-287
Q8H5N9 CSB-CF808450OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin PIP2-1 PIP2-1 1-290
Q84RL7 CSB-CF769568ZAX Recombinant Zea mays Aquaporin PIP2-1 PIP2-1 1-290
P43287 CSB-CF337371DOA Recombinant Arabidopsis thaliana Aquaporin PIP2-2 PIP2-2 2-285
Q6K215 CSB-CF757132OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin PIP2-2 PIP2-2 1-288
Q9ATM8 CSB-CF859754ZAX Recombinant Zea mays Aquaporin PIP2-2 PIP2-2 1-292
Q7XUA6 CSB-CF767132OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin PIP2-3 PIP2-3 1-290
Q9ATM7 CSB-CF859338ZAX Recombinant Zea mays Aquaporin PIP2-3 PIP2-3 1-289
P30302 CSB-CF329986DOA Recombinant Arabidopsis thaliana Aquaporin PIP2-3 PIP2-3 1-285
Q8GRT8 CSB-CF808400OFG Recombinant Oryza sativa subsp. japonica Aquaporin PIP2-4 PIP2-4 1-286
Q9ATM6 CSB-CF860580ZAX Recombinant Zea mays Aquaporin PIP2-4 PIP2-4 1-288
Q9FF53 CSB-CF863730DOA Recombinant Arabidopsis thaliana Probable aquaporin PIP2-4 PIP2-4 1-291
Q8GRI8 CSB-CF818101OFG Recombinant Oryza sativa subsp. japonica Aquaporin PIP2-5 PIP2-5 1-283
Q9XF58 CSB-CF893561ZAX Recombinant Zea mays Aquaporin PIP2-5 PIP2-5 1-285
Q9SV31 CSB-CF874534DOA Recombinant Arabidopsis thaliana Probable aquaporin PIP2-5 PIP2-5 1-286
Q9ZV07 CSB-CF104237DOA Recombinant Arabidopsis thaliana Probable aquaporin PIP2-6 PIP2-6 1-289
Q9ATM5 CSB-CF858955ZAX Recombinant Zea mays Aquaporin PIP2-6 PIP2-6 1-288
Q7XLR1 CSB-CF773519OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin PIP2-6 PIP2-6 1-282
P93004 CSB-CF310903DOA Recombinant Arabidopsis thaliana Aquaporin PIP2-7 PIP2-7 1-280
Q9ATM4 CSB-CF858338ZAX Recombinant Zea mays Aquaporin PIP2-7 PIP2-7 1-287
Q651D5 CSB-CF723826OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin PIP2-7 PIP2-7 1-290
Q9ZVX8 CSB-CF116052DOA Recombinant Arabidopsis thaliana Probable aquaporin PIP2-8 PIP2-8 1-278
Q7Y1E6 CSB-CF770295OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin PIP2-8 PIP2-8 1-280
Q7TNK7 CSB-CF756017RA Recombinant Rat Ammonium transporter Rh type A Rhag 1-450
O64425 CSB-CF526481DOA Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase RMA1 RMA1 1-249
Q6R567 CSB-CF761398DQD Recombinant Capsicum annuum E3 ubiquitin-protein ligase RMA1H1 RMA1H1 1-252
P93030 CSB-CF308618DOA Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase RMA2 RMA2 1-193
Q8GUK7 CSB-CF815421DOA Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase RMA3 RMA3 1-243
B3LTC4 CSB-CF455375SVP Recombinant Saccharomyces cerevisiae Putative uncharacterized protein SCRG_04940 SCRG_04940 1-152
A7A0K7 CSB-CF408175STA Recombinant Saccharomyces cerevisiae Putative uncharacterized protein SCY_3531 SCY_3531 1-152
Q5VR89 CSB-CF726109OFG Recombinant Oryza sativa subsp. japonica Aquaporin SIP1-1 SIP1-1 1-246
Q9M8W5 CSB-CF864961DOA Recombinant Arabidopsis thaliana Aquaporin SIP1-1 SIP1-1 1-240
Q9ATM3 CSB-CF860168ZAX Recombinant Zea mays Aquaporin SIP1-1 SIP1-1 1-245
Q9ATM2 CSB-CF861008ZAX Recombinant Zea mays Aquaporin SIP1-2 SIP1-2 1-243
Q9FK43 CSB-CF884269DOA Recombinant Arabidopsis thaliana Probable aquaporin SIP1-2 SIP1-2 1-243
Q9M1K3 CSB-CF862965DOA Recombinant Arabidopsis thaliana Probable aquaporin SIP2-1 SIP2-1 1-237
Q9ATM1 CSB-CF857689ZAX Recombinant Zea mays Aquaporin SIP2-1 SIP2-1 1-249
Q10M80 CSB-CF607470OFG Recombinant Oryza sativa subsp. japonica Aquaporin SIP2-1 SIP2-1 1-250
Q13336 CSB-CF615678HU Recombinant Human Urea transporter 1 SLC14A1 1-389
Q8VHL0 CSB-CF848706MO Recombinant Mouse Urea transporter 1 Slc14a1 1-384
Q99808 CSB-CF857875HU Recombinant Human Equilibrative nucleoside transporter 1 SLC29A1 2-456
P11166 CSB-CF021546HU Recombinant Human Solute carrier family 2, facilitated glucose transporter member 1 SLC2A1 1-492
Q9NP59 CSB-CF865089HU Recombinant Human Solute carrier family 40 member 1 SLC40A1 1-571
P28573 CSB-CF021706RA Recombinant Rat Sodium-dependent proline transporter Slc6a7 1-637
Q9P7T9 CSB-CF868457SXV Recombinant Schizosaccharomyces pombe Uncharacterized membrane protein C977.17 SPAC977.17 1-598
P25818 CSB-CF328569DOA Recombinant Arabidopsis thaliana Aquaporin TIP1-1 TIP1-1 1-251
P50156 CSB-CF342272OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin TIP1-1 TIP1-1 1-250
O64964 CSB-CF530171ZAX Recombinant Zea mays Aquaporin TIP1-1 TIP1-1 1-250
Q41963 CSB-CF670353DOA Recombinant Arabidopsis thaliana Aquaporin TIP1-2 TIP1-2 1-253
Q94CS9 CSB-CF853224OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin TIP1-2 TIP1-2 1-252
Q9ATM0 CSB-CF859753ZAX Recombinant Zea mays Aquaporin TIP1-2 TIP1-2 1-254
O82598 CSB-CF526874DOA Recombinant Arabidopsis thaliana Aquaporin TIP1-3 TIP1-3 1-252
Q41951 CSB-CF671454DOA Recombinant Arabidopsis thaliana Aquaporin TIP2-1 TIP2-1 1-250
Q7XA61 CSB-CF773505OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin TIP2-1 TIP2-1 1-248
Q9ATL9 CSB-CF860579ZAX Recombinant Zea mays Aquaporin TIP2-1 TIP2-1 1-249
Q5Z6F0 CSB-CF733508OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin TIP2-2 TIP2-2 1-248
Q41975 CSB-CF673709DOA Recombinant Arabidopsis thaliana Probable aquaporin TIP2-2 TIP2-2 1-250
Q9ATL8 CSB-CF859337ZAX Recombinant Zea mays Aquaporin TIP2-2 TIP2-2 1-250
Q84RL6 CSB-CF768011ZAX Recombinant Zea mays Aquaporin TIP2-3 TIP2-3 1-248
Q9FGL2 CSB-CF863746DOA Recombinant Arabidopsis thaliana Aquaporin TIP2-3 TIP2-3 1-250
P26587 CSB-CF326646DOA Recombinant Arabidopsis thaliana Aquaporin TIP3-1 TIP3-1 1-268
Q9FWV6 CSB-CF872332OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin TIP3-1 TIP3-1 1-264
Q9ATL7 CSB-CF858954ZAX Recombinant Zea mays Aquaporin TIP3-1 TIP3-1 1-262
Q9AT75 CSB-CF860166ZAX Recombinant Zea mays Aquaporin TIP3-2 TIP3-2 1-266
O22588 CSB-CF522785DOA Recombinant Arabidopsis thaliana Probable aquaporin TIP3-2 TIP3-2 1-267
Q7XKI5 CSB-CF801726OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin TIP3-2 TIP3-2 1-265
O82316 CSB-CF530555DOA Recombinant Arabidopsis thaliana Aquaporin TIP4-1 TIP4-1 1-249
Q9ATL6 CSB-CF858337ZAX Recombinant Zea mays Aquaporin TIP4-1 TIP4-1 1-255
Q75GA5 CSB-CF745044OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin TIP4-1 TIP4-1 1-251
Q9LWR0 CSB-CF864875OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin TIP4-2 TIP4-2 1-256
Q9ATL5 CSB-CF860167ZAX Recombinant Zea mays Aquaporin TIP4-2 TIP4-2 1-257
Q9ATL4 CSB-CF861007ZAX Recombinant Zea mays Aquaporin TIP4-3 TIP4-3 1-249
Q9LWR2 CSB-CF868056OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin TIP4-3 TIP4-3 1-251
Q9ATL3 CSB-CF857688ZAX Recombinant Zea mays Aquaporin TIP4-4 TIP4-4 1-252
Q7XU31 CSB-CF773728OFG Recombinant Oryza sativa subsp. japonica Probable aquaporin TIP5-1 TIP5;1 1-269
Q9STX9 CSB-CF874520DOA Recombinant Arabidopsis thaliana Probable aquaporin TIP5-1 TIP5-1 1-256
Q9ATL2 CSB-CF859752ZAX Recombinant Zea mays Aquaporin TIP5-1 TIP5-1 1-260
P25794 CSB-CF335149EWE Recombinant Pisum sativum Probable aquaporin PIP-type 7a TRG-31 1-289
Q54V53 CSB-CF690723DKK Recombinant Dictyostelium discoideum Aquaporin C wacA 1-274
P37327 CSB-CF330698ENV Recombinant Escherichia coli Inner membrane protein YfdC yfdC 1-310
P43549 CSB-CF337439SVG Recombinant Saccharomyces cerevisiae Uncharacterized membrane protein YFL054C YFL054C 1-646
P38750 CSB-CF327884SVG Recombinant Saccharomyces cerevisiae Uncharacterized transporter YHL008C YHL008C 1-627
P0CD98 CSB-CF315990SVG Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YLL053C YLL053C 1-152
O05399 CSB-CF515381BRJ Recombinant Bacillus subtilis Uncharacterized transporter YrhG yrhG 1-266
P39608 CSB-CF331049BRJ Recombinant Bacillus subtilis Uncharacterized transporter YwcJ ywcJ 1-256

アクアポリン研究用抗体

Uniprot No. 品番 Name Species Reactivity Tested Applications
P29972 CSB-PA001957GA01HU AQP1 Antibody Human, Mouse, Rat ELISA, WB, IHC
P29972 CSB-PA009568 AQP1 Antibody Human, Mouse, Rat ELISA, WB
P29972 CSB-PA076907 AQP1 Antibody Human, Mouse, Rat ELISA, IHC
P29972 CSB-PA118586 AQP1 Antibody Human ELISA, WB
P29972 CSB-PA16085A0Rb AQP1 Antibody Human, Mouse, Rat ELISA, WB, IF
P29972 CSB-PA16087A0Rb AQP1 Antibody Human ELISA, IHC, IF
P29972 CSB-PA990867 AQP1 Antibody Human, Mouse, Rat ELISA, WB, IHC
P29972 CSB-PA16085D0Rb AQP1 Antibody,Biotin conjugated Human ELISA
P29972 CSB-PA16087D0Rb AQP1 Antibody,Biotin conjugated Human ELISA
P29972 CSB-PA16085C0Rb AQP1 Antibody,FITC conjugated Human ELISA
P29972 CSB-PA16087C0Rb AQP1 Antibody,FITC conjugated Human ELISA
P29972 CSB-PA16085B0Rb AQP1 Antibody,HRP conjugated Human ELISA
P29972 CSB-PA16087B0Rb AQP1 Antibody,HRP conjugated Human ELISA
Q96PS8 CSB-PA693604 AQP10 Antibody Human ELISA, WB
Q96PS8 CSB-PA853482LA01HU AQP10 Antibody Human ELISA, IF
Q96PS8 CSB-PA853482LD01HU AQP10 Antibody,Biotin conjugated Human ELISA
Q96PS8 CSB-PA853482LC01HU AQP10 Antibody,FITC conjugated Human ELISA
Q96PS8 CSB-PA853482LB01HU AQP10 Antibody,HRP conjugated Human ELISA
Q8NBQ7 CSB-PA008895 AQP11 Antibody Human, Mouse, Rat ELISA, WB
Q8NBQ7 CSB-PA781210 AQP11 Antibody Human ELISA, IHC
Q8IXF9 CSB-PA972136 AQP12A Antibody Human ELISA, WB
Q8IXF9/A6NM10 CSB-PA000912 AQP12A/AQP12B Antibody Human ELISA, WB
P41181 CSB-PA000913 AQP2 Antibody Human, Mouse, Rat, Monkey ELISA, WB, IHC, IF
P41181 CSB-PA16069A0Rb AQP2 Antibody Human ELISA, IF
P41181 CSB-PA411017 AQP2 Antibody Human, Mouse, Rat ELISA, IHC
P41181 CSB-PA597642 AQP2 Antibody Human, Mouse, Rat ELISA, IHC
P41181 CSB-PA16069D0Rb AQP2 Antibody,Biotin conjugated Human ELISA
P41181 CSB-PA16069C0Rb AQP2 Antibody,FITC conjugated Human ELISA
P41181 CSB-PA16069B0Rb AQP2 Antibody,HRP conjugated Human ELISA
P41181 CSB-PA009580 Phospho-AQP2 (S256) Antibody Human, Mouse, Rat ELISA, IHC
Q92482 CSB-PA009603 AQP3 Antibody Human, Mouse, Rat ELISA, IHC
Q92482 CSB-PA13909A0Rb AQP3 Antibody Human ELISA, IHC, IF
Q92482 CSB-PA203676 AQP3 Antibody Human, Mouse, Rat ELISA, IHC
Q92482 CSB-PA531552 AQP3 Antibody Human, Mouse, Rat ELISA, WB, IHC
Q92482 CSB-PA13909D0Rb AQP3 Antibody,Biotin conjugated Human ELISA
Q92482 CSB-PA13909C0Rb AQP3 Antibody,FITC conjugated Human ELISA
Q92482 CSB-PA13909B0Rb AQP3 Antibody,HRP conjugated Human ELISA
P55087 CSB-PA000914 AQP4 Antibody Human, Mouse, Rat ELISA, WB, IHC
P55087 CSB-PA001964GA01HU AQP4 Antibody Human, Mouse, Rat ELISA, WB, IHC
P55087 CSB-PA579941 AQP4 Antibody Human, Mouse, Rat ELISA, WB, IHC
P55087 CSB-PA905782 AQP4 Antibody Human, Mouse, Rat ELISA, WB, IHC
P55064 CSB-PA000915 AQP5 Antibody Human ELISA, WB, IHC
P55064 CSB-PA191314 AQP5 Antibody Human ELISA, IHC
P55064 CSB-PA978373 AQP5 Antibody Human ELISA, IHC
Q13520 CSB-PA16139A0Rb AQP6 Antibody Human ELISA, IHC
Q13520 CSB-PA16139D0Rb AQP6 Antibody,Biotin conjugated Human ELISA
Q13520 CSB-PA16139C0Rb AQP6 Antibody,FITC conjugated Human ELISA
Q13520 CSB-PA16139B0Rb AQP6 Antibody,HRP conjugated Human ELISA
O14520 CSB-PA276755 AQP7 Antibody Human ELISA, IHC
O14520 CSB-PA390632 AQP7 Antibody Human ELISA, IHC
O94778 CSB-PA186529 AQP8 Antibody Human ELISA, IHC
O94778 CSB-PA905788 AQP8 Antibody Human ELISA, IHC
P30301 CSB-PA000910 MIP Antibody Human, Mouse, Rat ELISA, WB, IHC
P30301 CSB-PA013834LA01HU MIP Antibody Human ELISA, IHC, IF
P30301 CSB-PA013834LD01HU MIP Antibody,Biotin conjugated Human ELISA
P30301 CSB-PA013834LC01HU MIP Antibody,FITC conjugated Human ELISA
P30301 CSB-PA013834LB01HU MIP Antibody,HRP conjugated Human ELISA

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