記事ID ： 17794

神経変性疾患研究用抗体（Tau, AKAP4, APP) 神経変性疾患研究用抗体（Tau, AKAP4, APP)

メーカー名：SPQI Company

SPQI社は、神経変性疾患研究用抗体（Tau, AKAP4, APP）を提供しています。

商品ラインアップ

Tau抗体；
タウタンパク質は、単一の遺伝子MAPT（Microtubule-Associated Protein Tau）（遺伝子ID：4137）によってコードされており、微小管関連タンパク質のファミリーに属します。
AKAP4抗体；
AKAP4（Aキナーゼアンカータンパク質4）タンパク質は、プロテインキナーゼA（PKA）の調節サブユニットに結合し、またPKAホロ酵素を細胞内の別々の位置に閉じ込めるという機能をもつAキナーゼアンカータンパク質（AKAP）ファミリーに属します。ヒトAKAP4遺伝子はX染色体上に位置し、精子鞭毛の線維鞘に局在する854アミノ酸ポリペプチドをコードし、AKAP4は精子の運動性に重要な役割を果たします。 AKAP4は、AKAP-4、AKAP82（A-キナーゼアンカータンパク質82KDa）、PRKA4（プロテインキナーゼAアンカータンパク質4）、HI、CT99（癌/ Testis抗原99）、FSC1（線維鞘成分1）またはP82とも呼ばれています。
APP抗体；
APP（遺伝子ID：351）とも呼ばれるアミロイドタンパク質前駆体は、1型膜貫通タンパク質であり、その代謝がアミロイド-βペプチドの産生をもたらします。アルツハイマー病では、アミロイドペプチドがアミロイド沈着の主成分であり。アミロイド - ベータペプチド（Ab）は、セクレターゼと呼ばれるプロテアーゼによってAPPの逐次的切断に続いて産生されます。

Tau抗体

Tau [pS422] 抗体 (clone 2H9)
セリン422でのリン酸化は、病理学的エピトープであり、神経原線維変性マーカー、アルツハイマー病の神経病理学的特徴の1つであるである。脳組織切片において、抗タウpS422クローン2H9抗体（4BDX-1501）は、神経原線維変性に関与するニューロンを特異的に検出する。脳組織切片において、抗タウpS422クローン2H9抗体（4BDX-1501）は、神経原線維変性に関与するニューロンを特異的に検出します。
セリン422は、以下のキナーゼによってリン酸化されます：CaMKII（カルシウムカルモジュリンキナーゼII）、PKA（プロテインキナーゼA）、JNK（c-Jun N末端キナーゼ）、ERK1 / 2またはp42 / 44 MAKP（マイトジェン活性化キナーゼ）、p38MAPKおよびMARK（微小管関連タンパク質/微小管親和性調節キナーゼ）。

品番	抗体名	特異性	用途	免疫動物	交差性
4BDX-1501 4BDX-1501S	Anti-Tau [pS422] mAb (clone 2H9)	Anti-Tau phosphorylated at serine 422	WB, IF, IHC, FCM	Mouse	Human, Mouse, Rat

図1：品番：4BDX-1501、ヒト脳組織を
用いたウエスタンブロット

図2：品番：4BDX-1501、神経原線維変性の
トランスジェニックモデルの免疫組織染色図

図3：品番：4BDX-1501、神経原繊維変性の
トランスジェニックモデルの免疫蛍光染色図

Tau [pS199]抗体
セリン199でのリン酸化は、神経変性プロセスの初期に観察される。神経原線維変性は、アルツハイマー病および前頭側頭型痴呆を含むいくつかの神経変性疾患において観察される一般的な神経病理学的病変である。　タウのセリン199は、以下のキナーゼによってリン酸化されます：CDK5（サイクリン依存性キナーゼ5）、CK2（カゼインキナーゼ2）、PKA（プロテインキナーゼA）、GSK3（グリコーゲンシンターゼキナーゼ3）、ERK / MAPK（マイトジェン活性化キナーゼ）およびMARK（微小管関連タンパク質/微小管親和性調節キナーゼ）。

品番	抗体名	特異性	用途	免疫動物	交差性
4BDX-1502 4BDX-1502S	Anti-Tau [pS199]	Anti-Tau phosphorylated at serine 199	WB, IHC, IF	Rabbit	Human, Mouse, Rat

図4：品番：4BDX-1502、Tau発現細胞を用いた
ウエスタンブロット

図5：品番：4BDX-1502、アルツハイマー脳組織の
神経原変性組織の免疫組織染色図

図6：4BDX-1502、マウスにおける高リン酸化型Tauの
免疫蛍光染色図

【製品使用文献】

・品番：4BDX-1501、4BDX-1501S▼

Lam S, Petit F, Hérard AS, Boluda S, Eddarkaoui S, Guillermier M, The Brainbank Neuro-CEB Neuropathology Network, Buée L, Duyckaerts C, Haik S, Picq JL, Dhenain M (2021) Amyloid and tau pathologies, cognitive impairments and cerebral atrophy after Alzheimer brain extracts inoculation in a primate. Acta Neuropathol Commun. in press. Doi: 10.1186/s40478-021-01266-8
Lam S, Boluda S, Herard AS, Petit F, Eddarkaoui S,Cambon K, The Brainbank Neuro-CEB Neuropathology Network, Picq JL, Buee L, Duyckaerts C, Haik S and Dhenain M (2021) Alzheimer’s brain inoculation in As-plaque bearing mice: synaptic loss is linked to tau seeding and low microglial activity. BioRxiv preprint doi: https://doi.org/10.1101/2021.04.06.438654;
Tautou M, Eddarkaoui S, Descamps F, Larchanche P-E, El Bakali J, Goveas LM, Dumoulin M, Lamarre C, Blum D, Buee L, Melnyk P and Sergeant N (2021) A s-Secretase Modulator Decreases Tau Pathology and Preserves Short-Term Memory ina Mouse Model of Neurofibrillary Degeneration. Front. Pharmacol. Vol 12:679335. doi: 10.3389/fphar.2021.679335
Troquier L, Caillierez R, Burnouf S, Fernandez-Gomez FJ, Grosjean ME, Zommer N, Sergeant N, Schraen-Maschke S, Blum D and Buee L (2012) Targeting phospho-Ser422 by active Tau Immunotherapy in the THY-Tau22 mouse model: a suitable therapeutic approach. Curr Alzheimer Res. 9(4):397-405. doi: 10.2174/156720512800492503.
Sergeant N, Vingtdeux V, Eddarkaoui S, Gay M, Evrard C, Le Fur N, Laurent C, Caillierez R, Obriot H, Larchanche PE, Farce A, Coevoet M, Carato P, Kouach M, Descat A, Dallemagne P, Buee-Scherrer V, Blum D, Hamdane M, Buee L, Melnyk P (2019). New piperazine multi-effect drugs prevent neurofibrillary degeneration and amyloid deposition, and preserve memory in animal models of Alzheimer's disease. Neurobiol Dis. Vol. 129:217-233. doi: 10.1016/j.nbd.2019.03.028.
Sergeant N, Bretteville A, Hamdane M, Caillet-Boudin ML, Grognet P, Bombois S, Blum D, Delacourte A, Pasquier F, Vanmechelen E , Schraen-Maschke S, and Buee L (2008) Biochemistry of Tau in Alzheimer’s disease and related neurological disorders. Expert review of proteomics 5:207?224. Doi: 10.1586/14789450.5.2.207

・品番：4BDX-1502、4BDX-1502S▼

品名	メーカー	品番	包装	希望販売価格
Anti τ, phospho Ser422, (Mouse) , 2H9	SPQ	4BDX-1501	100 UG	￥76,000
Anti τ, phospho Ser422, (Mouse) , 2H9	SPQ	4BDX-1501S	20 UG	￥48,000
Anti τ, phospho Ser199 (Rabbit)	SPQ	4BDX-1502	80 UL	￥63,000
Anti τ, phospho Ser199 (Rabbit)	SPQ	4BDX-1502S	40 UL	￥38,000

AKAP4抗体

AKAP4抗体
AKAP4（Aキナーゼアンカータンパク質4）タンパク質は、プロテインキナーゼA（PKA）の調節サブユニットに結合し、またPKAホロ酵素を細胞内の別々の位置に閉じ込めるという機能をもつAキナーゼアンカータンパク質（AKAP）ファミリーに属します。ヒトAKAP4遺伝子はX染色体上に位置し、精子鞭毛の線維鞘に局在する854アミノ酸ポリペプチドをコードし、AKAP4は精子の運動性に重要な役割を果たします。 AKAP4は、AKAP-4、AKAP82（A-キナーゼアンカータンパク質82KDa）、PRKA4（プロテインキナーゼAアンカータンパク質4）、HI、CT99（がん/ Testis抗原99）、FSC1（線維鞘成分1）またはP82とも呼ばれています。

品番	抗体名	特異性	用途	免疫動物	交差性
4BDX-1602 4BDX-1602S	Anti-AKAP4 (clone 7E10)	C-ter	WB, IF, EM, IHC, FCM	Mouse	Human, Horse,Bull, Mouse,Ram, Rat
4BDX-1805 4BDX-1805S	Anti-AKAP4 (clone 10F8)	C-ter	WB, IF, IHC, FCM	Mouse	Dog, Rabbit,Pig, Ram, Goat
4BDX-1701 4BDX-1701S	Anti-proAKAP4 (clone 6F12)	AKAP4 Prodomain	WB, IF, IHC, FCM	Mouse	Human, Horse,Bull, Mouse,Ram, Rat
4BDX-1901 4BDX-1901S	Anti-proAKAP4 (clone 5H2)	AKAP4 Prodomain	WB, IF, IHC, FCM	Mouse	Goat, Camel, Cat, Dog, Pig, Rabbit, Human

図7 clone 7E10のヒト精子の免疫蛍光染色、品番：48DX-1602

図8 clone 7E10のヒト睾丸組織の免疫組織染色、品番：48DX-1602

図9 clone 6F12のヒト精子の免疫蛍光染色、品番：4BDX-1701

【製品使用文献】

・品番：4BDX-1602、4BDX-1602S▼

Blommaert D, Franck T, Lejeune JF, Selleslagh M, Jouy N, Sergeant N, Serteyn D and Delehedde M (2019b) The Horse 4MIDR Kit as a promising tool to compare different freezing media to preserve stallion semen. Rev. Bras. Reprod. Anim. Vol 43(2):585.
Benderradji H, Anne-Laure Barbotin, Maryse Leroy-Billiard, Julie Prasivoravong, François Marcelli, Christine Decanter, Geoffroy Robin, Valérie Mitchell, Jean-Marc Rigot, Antonino Bongiovanni, Florent Sauve, Luc Buée, Claude-Alain Maurage, Maryse Cartigny, Arnauld Villers, Vincent Prevot, Sophie Catteau-Jonard, Nicolas Sergeant , Paolo Giacobini, Pascal Pigny, Clara Leroy(2022) Defining Reference Ranges for Serum Anti-Müllerian Hormone on a Large Cohort of Normozoospermic Adult Men Highlights New Potential Physiological Functions of AMH on FSH Secretion and Sperm Motility. J Clin Endocrinol Metab.16;107(7):1878-1887. doi: 10.1210/clinem/dgac218.
Blommaert D, Sergeant N, Delehedde M, Jouy N, Mitchell V, Franck T, Donnay I, Lejeune JP and Serteyn D (2019b) Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology. Vol. 131:52-60. Doi: 10.1016/j.theriogenology.2019.03.011
Carracedo S, Lamia Briand-Amirat, Marta Dordas-Perpinyà, Yolanda Ramos Escuredo, Romain Delcombel, Nicolas Sergeant, Maryse Delehedde (2022)ProAKAP4 protein marker: Towards a functional approach to male fertility.Anim Reprod Sci.247:107074. doi: 10.1016/j.anireprosci.2022.107074.
Carracedo S, Loyens A, Eddarkaoui S, Serteyn D, Malo C, Skidmore L, Briand-Amirat L, Barbotin AL, Maurage CA, Delehedde M and Sergeant N (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations, and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448 https://www.sciencedirect.com/science/article/abs/pii/S0378432020303201
Delehedde M, Carracedo S, Duchene B, Demouveaux B, Remy G, Kervoaze G, Aubry S, Gevaert MH, Maurage CA, Jonckheere N, Van Seuningen I, Philippe Gosset G, Desseyn JL, Sergeant N and Pichavant M (2020) Cigarette smoking affects the sperm-specific proAKAP4 concentrations and impairs both spermatogenesis and sperm quality. Andrology. Vol. 8 (Suppl. 1):45-46. Doi: 10.1111/andr.12906
Delehedde M, Carracedo S, Selleslagh M, Eddarkaoui S, Amirat-Briand L and Sergeant N (2019) ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. Vol. 2(1):13-19. https://ologyjournals.com/ijgrs/ijgrs_00007.pdf
Delehedde M, Demouveaux B, Remy G, Selleslagh M, Dewulf Q, Desseyn JL, Moreau M, Gosset P, Muriel Pichavant M and Sergeant N (2019a) ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. Vol. 7(1):86. Doi: 10.1111/andr.12624
Delehedde M, Didier Bloomaert, Nathalie Jouy, Julie Scabello, Hortense Miersman, Thierry Franck, Didier Serteyn, Valérie Mitchell, Nicolas Sergeant(2018) Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. Animal Reproduction ScienceVolume 194, July 2018, Page e24
Dordas-Perpinya M(2022) ProAKAP4 correlation with motion parameters and spermatozoa subpopulations in stallion.Reproduction in Domestic Animals. Vol57,4:60-61.
Dordas-Perpinya M(2022)ProAKAP4 concentrations in stallion and donkey: Comparison with kinetic parameters, motility and concentration. Reproduction in Domestic Animals. Vol57;1,61.
Dordas-Perpinya M, N. Sergeant, I. Yanez-Ortiz, J. Catalan, M. Delehedde, J. Bruyas, L. Briand-Amirat and J. Miro(2022)Characterization of proAKAP4 expression, metabolism and localization in Equus asinus spermatozoa.Vol35; 2, 215-216.
Dordas-Perpinyà M, Nicolas Sergeant, Isabelle Ruelle, Jean-Francois Bruyas, Frédéric Charreaux, Sandrine Michaud, Sara Carracedo, Jaime Catalán, Jordi Miró, Maryse Delehedde, Lamia Briand-Amirat(2022) ProAKAP4 Semen Concentrations as a Valuable Marker Protein of Post-Thawed Semen Quality and Bull Fertility: A Retrospective Study. Vet Sci. 2022 May 6;9(5):224. doi: 10.3390/vetsci9050224.
Fatet (2022) The sperm specific protein proAKAP4 as a marker to evaluate sperm quality and fertility in Bucks. Reproduction in Domestic Animals. Vol57; 4, 79.
Griffin R, Swegen A, Baker M, John Aitken R, A Skerrett-Byrne D, Silva Rodriguez A, E Martin-Cano F, Nixon B, J Pena F, Delehedde M, Sergeant N and Gibb Z (2020) Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa. Reproduction. 160(5):695-707. Doi: 10.1530/REP-20-0284.
Langlade C, Buff S, Dias C, Commin L (2020) Assessment of Optimized Frozen/Thawed Semen Samples in Canines with the New A-Kinase Anchor Protein 4 Precursor Biomarker. Biopreserv Biobank. 18(5):409-414. doi: 10.1089/bio.2020.0019.
Le Couazer (2019) Expression of proAKAP4 in dog semen as promising marker of sperm quality. Reproduction in Domestic Animal. Vol54; S3, 73.
Le Couazer, M Delehedde, I Ruelle, N Sergeant, S Michaud, L Briand, D Bencharif(2019) ProAKAP4 as a valuable marker to assess sperm quality in dogs. Reproduction in Domestic Animals. Vol54; 2, 91-92.
Malo C, Sara Carracedo, Maryse Delehedde, Nicolas Sergeant, Julian Alexandra Skidmore (2021) Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters. Reproduction and Fertility Vol2; 4, 268-279.
Nixon B, Ilana R Bernstein, Shenae L Cafe, Maryse Delehedde, Nicolas Sergeant, Amanda L Anderson, Natalie A Trigg, Andrew L Eamens, Tessa Lord, Matthew D Dun, Geoffry N De Iuliis, Elizabeth G Bromfield (2019) A Kinase Anchor Protein 4 Is Vulnerable to Oxidative Adduction in Male Germ Cells. Frontiers in Cell and Developmental Biology Vol7, 319.
Riesco M, Anel-Lopez L, Neila-Montero M, Palacin-Martinez C, Montes-Garrido R, Alvarez M, de Paz P, Anel L (2020) ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules. 10(7):1046. doi: 10.3390/biom10071046.
Ruelle I (2019) Assessment of the sperm specific protein proAKAP4 as a marker to evaluate sperm quality and fertility in Holstein bulls. Rev. Bras. Reprod. Anim. Vol43; 2, 472.
Ruelle I, Sergeant N, Bencharif D, Charreaux F, Thorin C, Michaud S, Dordas-Perpinya M, Jouy N, Audry S, Maurage CA, Delehedde M and Briand-Amirat L (2020) ProAKAP4 concentrations in semen as a predictive tool of bull fertility: A preliminary study. Reproduction, Fertility and Development. 32(1):145. https://www.bvs-vet.org.br/vetindex/periodicos/revista-brasileira-de-reproducao-animal/43-(2019)-2/
Scovell JM, Bournat JC, Szafran AT, Solis M, Moore J, Rivera A, Chen CH, Zhang J, Wilken N, Seth A, Jorgez CJ (2021) PRSS50 is a testis protease responsible for proper sperm tail formation and function. Development Vol.148 (8): dev197558. Doi: 10.1242/dev.197558
Sergeant N, Blommaert D, Carracedo S, Franck T, Lejeune JP, Gevaert MH, Aubry S, Maurage CA, Serteyn D and Delehedde M (2020) Assessment of the proAKAP4 concentrations as a pertinent sperm parameter to select extenders and preservatives of stallion semen. Animal Reproduction Science. Vol. 220 :106449. P89. https://www.sciencedirect.com/science/article/abs/pii/S0378432020303213#!
Sergeant N, Carracedo S, Blommaert D, Aubry S, Jouy N, Lejeune JP, Franck T, Maurage CA, Serteyn D, Buee L and Delehedde M (2020) Proteolysis of proAKAP4 in semen as a regulatory sensor of sperm quality and functionality. Andrology. Vol. 8 (Suppl. 1):44-45. Doi: 10.1111/andr.12906
Sergeant N, F. Jumeau, S. Eddarkaoui, J. Sigala, Gbete Florian Dossou, M. Delehedde, L. Buee, Jean Francois Yvoz, V. Mitchell (2016) Investigating proteomic methods and tools to assess sperm quality. Animal Reproduction Science Vol169, 99-135.
Sergeant N, Lamia Briand-Amirat, Djemil Bencharif and Maryse Delehedde (2019) The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol11, 43466.

・品番：4BDX-1701、4BDX-1701S▼

Blommaert D, Franck T, Lejeune JF, Selleslagh M, Jouy N, Sergeant N, Serteyn D and Delehedde M (2019b) The Horse 4MIDR Kit as a promising tool to compare different freezing media to preserve stallion semen. Rev. Bras. Reprod. Anim. Vol 43(2):585. https://www.febrasgo.org.br/media/k2/attachments/RBGOZ43Z5.pdf
Blommaert D, N. Sergeant, M. Delehedde, T. Franck, J. Lejeune, D. Serteyn (2018) Significant Correlation Between the proAKAP4 Concentration and the Total and Progressive Motility in Stallion Sperm After Thawing. Journal of Equine Veterinary Science Vol.66, 43.
Blommaert D, Sergeant N, Delehedde M, Jouy N, Mitchell V, Franck T, Donnay I, Lejeune JP and Serteyn D (2019) Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology. Vol. 131:52-60. Doi: 10.1016/j.theriogenology.2019.03.011.
Carracedo S, Lamia Briand-Amirat, Marta Dordas-Perpinyá, Yolanda Ramos Escuredo, Romain Delcombel, Nicolas Sergeant, Maryse Delehedde (2022)ProAKAP4 protein marker: Towards a functional approach to male fertility.Anim Reprod Sci.247:107074. doi: 10.1016/j.anireprosci.2022.107074.
Carracedo S, Loyens A, Eddarkaoui S, Serteyn D, Malo C, Skidmore L, Briand-Amirat L, Barbotin AL, Maurage CA, Delehedde M and Sergeant N (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448 https://www.sciencedirect.com/science/article/abs/pii/S0378432020303201
Delehedde M, Carracedo S, Duchene B, Demouveaux B, Remy G, Kervoaze G, Aubry S, Gevaert MH, Maurage CA, Jonckheere N, Van Seuningen I, Philippe Gosset G, Desseyn JL, Sergeant N and Pichavant M (2020) Cigarette smoking affects the sperm-specific proAKAP4 concentrations and impairs both spermatogenesis and sperm quality. Andrology. Vol. 8 (Suppl. 1):45-46. https://doi.org/10.1111/andr.13044
Delehedde M, Carracedo S, Selleslagh M, Eddarkaoui S, Amirat-Briand L and Sergeant N (2019) ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. Vol. 2(1):13-19. Doi: 10.1111/andr.12906
Delehedde M, Demouveaux B, Remy G, Selleslagh M, Dewulf Q, Desseyn JL, Moreau M, Gosset P, Muriel Pichavant M and Sergeant N (2019) ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. Vol. 7(1):86. https://doi.org/10.1111/andr.12624
Delehedde M, Didier Bloomaert, Nathalie Jouy, Julie Scabello, Hortense Miersman, Thierry Franck, Didier Serteyn, Valerie Mitchell, Nicolas Sergeant(2018) Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. Animal Reproduction ScienceVolume 194, July 2018, Page e24
Dordas-Perpinya M(2022) ProAKAP4 correlation with motion parameters and spermatozoa subpopulations in stallion.Reproduction in Domestic Animals. Vol57,4:60-61.
Dordas-Perpinya M(2022)ProAKAP4 concentrations in stallion and donkey: Comparison with kinetic parameters, motility and concentration. Reproduction in Domestic Animals. Vol57;1,61.
Dordas-Perpinya M, N. Sergeant, I. Yanez-Ortiz, J. Catalan, M. Delehedde, J. Bruyas, L. Briand-Amirat and J. Miro(2022)Characterization of proAKAP4 expression, metabolism and localization in Equus asinus spermatozoa.Vol35; 2, 215-216.
Dordas-Perpinyà M, Nicolas Sergeant, Isabelle Ruelle, Jean-Francois Bruyas, Frédéric Charreaux, Sandrine Michaud, Sara Carracedo, Jaime Catalán, Jordi Miró, Maryse Delehedde, Lamia Briand-Amirat(2022) ProAKAP4 Semen Concentrations as a Valuable Marker Protein of Post-Thawed Semen Quality and Bull Fertility: A Retrospective Study. Vet Sci. 2022 May 6;9(5):224. doi: 10.3390/vetsci9050224.
Fatet (2022) The sperm specific protein proAKAP4 as a marker to evaluate sperm quality and fertility in Bucks. Reproduction in Domestic Animals. Vol57; 4, 79.
Griffin R, Swegen A, Baker M, John Aitken R, A Skerrett-Byrne D, Silva Rodriguez A, E Martin-Cano F, Nixon B, J Pena F, Delehedde M, Sergeant N and Gibb Z (2020) Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa. Reproduction. 160(5):695-707. doi: 10.1530/REP-20-0284.
Langlade C, Buff S, Dias C, Commin L (2020) Assessment of Optimized Frozen/Thawed Semen Samples in Canines with the New A-Kinase Anchor Protein 4 Precursor Biomarker. Biopreserv Biobank. 18(5):409-414. doi: 10.1089/bio.2020.0019.
Le Couazer (2019) Expression of proAKAP4 in dog semen as promising marker of sperm quality. Reproduction in Domestic Animal. Vol54; S3, 73.
Le Couazer, M Delehedde, I Ruelle, N Sergeant, S Michaud, L Briand, D Bencharif(2019) ProAKAP4 as a valuable marker to assess sperm quality in dogs. Reproduction in Domestic Animals. Vol54; 2, 91-92.
Malo C, Sara Carracedo, Maryse Delehedde, Nicolas Sergeant, Julian Alexandra Skidmore (2021) Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters. Reproduction and Fertility Vol2; 4, 268-279.
Nixon B, Ilana R Bernstein, Shenae L Cafe, Maryse Delehedde, Nicolas Sergeant, Amanda L Anderson, Natalie A Trigg, Andrew L Eamens, Tessa Lord, Matthew D Dun, Geoffry N De Iuliis, Elizabeth G Bromfield (2019) A Kinase Anchor Protein 4 Is Vulnerable to Oxidative Adduction in Male Germ Cells. Frontiers in Cell and Developmental Biology Vol7, 319.
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品名	メーカー	品番	包装	希望販売価格
Anti AKAP4, (Mouse) , 7E10	SPQ	4BDX-1602S	20 UG	￥64,000
Anti AKAP4, (Mouse) , 7E10	SPQ	4BDX-1602	100 UG	￥101,000
Anti AKAP4, (Mouse) , 10F8	SPQ	4BDX-1805S	20 UG	￥48,000
Anti AKAP4, (Mouse) , 10F8	SPQ	4BDX-1805	100 UG	￥76,000
Anti proAKAP4, (Mouse) , 6F12	SPQ	4BDX-1701S	20 UG	￥64,000
Anti proAKAP4, (Mouse) , 6F12	SPQ	4BDX-1701	100 UG	￥101,000
Anti proAKAP4, (Mouse) , 5H2	SPQ	4BDX-1901S	20 UG	￥48,000
Anti proAKAP4, (Mouse) , 5H2	SPQ	4BDX-1901	100 UG	￥76,000

APP抗体

APP [pT668] 抗体
スレオニン668（APP695アイソフォームに対応する位置）でのリン酸化は、アルツハイマー病において増加し、アミロイド - ベータペプチド産生を促進します。　スレオニン668は、in vitro、in vivo のどちらにおいても以下のキナーゼによってリン酸化されます：グリコーゲンシンターゼキナーゼ3β（GSK3β）、Jun N末端キナーゼ-3（JNK3）、Jun N末端キナーゼ1（JNK1）、細胞分裂サイクルタンパク質（Cdc2）、サイクリン依存性キナーゼ5（Cdk5）、およびDYRK1A（二重特異性チロシン（Y）リン酸化調節キナーゼ1A)。