濃縮タイプと Ready-to-Use タイプをご用意 免疫病理学用抗体（サイトケラチン／アクチン／クロモグラニンAなど）

品番：ENZ30902 / ENZC34902
クローン：35bH11
抗原：Low MW Cytokeratin [8]
サブタイプ：Mouse IgM
適用：IHC, IB
交差種：Human
免疫原の調製：ヒト肝細胞癌セルライン Hwp3B 由来の Triton/High Salt-不溶性物質

染色図

参考文献
1. Battifora, H. and Kopinski, M. (1986) The influence of protease digestion and duration of fixation on the immunostaining of keratins. A comparison of formalin and ethanol fixation. J. Histochem. Cytochem. 34:1095-1100.
2. Bolen, J. W. and McNutt, M. A. (1987) Cytoskeletal intermediate filaments: practical applications of intermediate filament analysis. Ultrastruct. Pathol. 11:175-189.
3. Gown, A. M. and Vogel, A. M. (1982) Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies. J. Cell Biol. 95:414-424.
4. Gown, A. M. and Vogel, A. M. (1984) Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues. Am. J. Path. 114:309-321.
5. Gown, A. M. and Vogel, A. M. (1985) Monoclonal antibodies to human intermediate filament proteins. III. Analysis of tumors. Am. J. Clin. Path. 84:413-422.

品番：ENZ30903 / ENZC34903
クローン：34bE12
抗原：High MW Cytokeratin-903 [1, 5, 10, 14]
サブタイプ：Mouse IgG1
適用：IHC, IB
交差種：Human
免疫原の調製：ヒト角質由来可溶性ケラチン [足の角質層由来の 8M urea では不溶性だが、8M urea/ 0.1M b-mercaptoethanol/5% SDS では可溶性のタンパク質]

染色図

参考文献
1. Battifora, H. and Kopinski, M. (1986) The influence of protease digestion and duration of fixation on the immunostaining of keratins. A comparison of formalin and ethanol fixation. J. Histochem. Cytochem. 34:1095-1100.
2. Gown, A. M. and Vogel, A. M. (1982) Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies. J. Cell Biol. 95:414-424.
3. Gown, A. M. and Vogel, A. M. (1984) Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues. Am. J. Path. 114:309-321.
4. Gown, A. M. and Vogel, A. M. (1985) Monoclonal antibodies to human intermediate filament proteins. III. Analysis of tumors. Am. J. Clin. Path. 84:413-422.
5. Grignon, D. J., Ro, J. Y., Ordonez, N. G., Ayala, A. G. and Cleary, K. R. (1988) Basal cell hyperplasia, adenoid basal cell tumor and adenoid cystic carcinoma of the prostate gland. Human Pathol. 19:1425-1433.
6. Hedrick, L. and Epstein, J. I. (1989) Use of Keratin 903 as an adjunct in the diagnosis of prostate carcinoma. Am. J. Surg. Pathol. 13:389-396.

品番：ENZ30904 / ENZC34904
クローン：34bB4
抗原：High MW Cytokeratin
サブタイプ：Mouse IgG1
適用：IHC
交差種：Human
免疫原の調製：ヒト角質由来可溶性ケラチン [足の角質層由来の 8M urea では不溶性だが、8M urea/ 0.1M b-mercaptoethanol/5% SDS では可溶性のタンパク質]

染色図

参考文献
1. Battifora, H. and Kopinski, M. (1986) The influence of protease digestion and duration of fixation on the immunostaining of keratins. A comparison of formalin and ethanol fixation. J. Histochem. Cytochem. 34:1095-1100.
2. Gown, A. M. and Vogel, A. M. (1982) Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies. J. Cell Biol. 95:414-424.
3. Gown, A. M. and Vogel, A. M. (1984) Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues. Am. J. Path. 114:309-321.
4. Gown, A. M. and Vogel, A. M. (1985) Monoclonal antibodies to human intermediate filament proteins. III. Analysis of tumors. Am. J. Clin. Path. 84:413-422.

品番：ENZ30930 / ENZC34930
クローン：HMB45
抗原：Melanoma-specific antigen
サブタイプ：Mouse IgG1
適用：IHC
交差種：human
免疫原の調製：色素性黒色腫転移を含むヒト腋窩リンパ節

染色図

参考文献
1. Duray, P. H., Palazzo, J., Gown, A. M. and Ohuchi, N. (1988) Melanoma cell heterogeneity: a study of two monoclonal antibodies compared with S-100 protein in paraffin sections. Cancer 61:2460-2468.
2. Gown, A. M., et al. (1985) Monoclonal antibodies to a melanoma-specific cytoplasmic antigen. Lab. Invest. 52:25A.
3. Gown, A. M., Vogel, A. M., Hoak, D., Goush, F. and McNutt, M. A. (1986) Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes. Am. J. Path. 123:195-203.
4. Ordonez, N. G., Xiaolong, J. and Hickey, R. C. (1988) Comparison of HMB-45 monoclonal antibody and S-100 protein in the immunohistochemical diagnosis of melanoma. Am. J. Clin. Path. 90:385-390.
5. Wick, M. R., Stanley, S. J. and Swanson, P. E. (1988) Immunohistochemical diagnosis of sinonasal melanoma, carcinoma, and neuroblastoma with monoclonal antibodies HMB-45 and anti-synaptophysin. Arch. Path. Lab. Med. 112:616-620.
6. Wick, M. R., Swanson, P. E. and Rocamora, A. (1988) Recognition of malignant melanoma by monoclonal antibody HMB-45. An immunohistochemical study of 200 paraffin-embedded cutaneous tumors. J. Cutan. Path. 15:201-207.

品番：ENZ30931 / ENZC34931
クローン：HHF35
抗原：Muscle Actin (actin-a and actin-g)
サブタイプ：Mouse IgG1
適用：IHC, IB
交差種：human, monkey, rabbit
免疫原の調製：ヒト心筋 [2%SDS で抽出されたタンパク質]

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参考文献
1. Charbord, P., Gown, A. M., Keating, A. and Singer, J. W. (1985) CGA-7 and HHF, two monoclonal antibodies that recognize muscle actin and react with adherent cells in human long-term bone marrow cultures. Blood 66:1138-1142.
2. Gown, A. M., Tsukada, T. and Ross, R. (1986) Human atherosclerosis. II. Immunocytochemical analysis of the cellular composition of human atherosclerotic lesions. Am. J. Pathol. 125:191-207.
3. Schmidt, R. A. Cone, R., Haas, J. E. and Gown, A. M. (1987) Diagnosis of Rhabdomyosarcomas using HHF35, an anti-muscle actin monoclonal antibody. Lab. Invest. 56:70A.
4. Schmidt, R. A. Cone, R., Haas, J. E. and Gown, A. M. (1988) Diagnosis of Rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins. Am. J. Path. 131:19-28.
5. Tsukada, T., et al. (1986) HHF35, a muscle actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization. Am. J. Path. 126:51-60.
6. Tsukada, T., et al. (1987) HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive and neoplastic human tissues. Am. J. Path. 127:389-402.

品番：ENZ30932 / ENZC34932
クローン：PHE
抗原：Chromogranin A in neuroendocrine cells
サブタイプ：Mouse IgG1
適用：IHC
交差種：Human
免疫原の調製：ヒト褐色細胞腫

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参考文献
1. Riddell, K., Tippens, D. and Gown, A. M. (1987) PHE5, a new monoclonal antibody to a unique neuroendocrine granule protein. Lab. Invest. 56:64A.
2. Lloyd, R. V., et al. (1988) Distribution of Chromogranin A and Secretogranin I (Chromogranin B) in Neuroendocrine Cells and Tumors. Am. J. Pathol. 130:296-304.
3. Bordi, C., Pilato, E. P. and D’Adda, T. (1988) Comparative study of seven neuroendocrine markers in pancreatic endocrine tumours. Virchows Archiv. A. Path. Anat. 413:387-398.
4. Papotti, M., et al. (1989) Neuroendocrine differentiation in carcinomas of the breast: a study of 51 cases. Sem. in Diag. Path. 6:174-188.

品番：ENZ30933 / ENZC34933
クローン：CGA7
抗原：Smooth Muscle Actin (actin-a and actin-g)
サブタイプ：Mouse IgG2b
適用：IHC, IB
交差種：chicken, rabbit, monkey, human
免疫原の調製：部分的に精製したニワトリ砂嚢のアクチン

染色図

参考文献
1. Charbord, P., Gown, A. M., Keating, A. and Singer, J. W. (1985) CGA-7 and HHF, two monoclonal antibodies that recognize muscle actin and react with adherent cells in human long-term bone marrow cultures. Blood 66:1138-1142.
2. Gown, A. M., Vogel, A. M., Gordon, D. and Lu, P. L. (1985) A smooth muscle-specific monoclonal antibody recognizes smooth muscle actin isozymes. J. Cell Biol. 100:807-813.
3. Tsukada, T., et al., (1985) Smooth muscle cell and macrophage specific monoclonal antibodies in the immunocytochemical analysis of human and rabbit atherosclerotic lesions. Circulation 72 (Suppl. III):34.

品番：ENZ30935 / ENZC34935
クローン：HAM56
抗原：Macrophage
サブタイプ：Mouse IgM
適用：IHC
交差種：human, monkey
免疫原の調製：ソニケートしたヒト肺胞マクロファージ

染色図

参考文献
1. Gown, A. M., Tsukada, T. and Ross, R. (1986) Human atherosclerosis. II. Immunocytochemical analysis of the cellular composition of human atherosclerotic lesions. Am. J. Pathol. 125:191-207.
2. Alpers, C. E., Magil, A. B. and Gown, A. M. (1989) Macrophage origin of the multinucleated cells of myeloma cast nephropathy. Am. J. Clin. Pathol. 92:662-665.