記事ID ： 33580

オリゴヌクレオチド、ペプチドへの標識また各FRETのドナー構築に最適な蛍光試薬オリゴヌクレオチド標識試薬 Tide Fluor™ Dye

メーカー名：AAT Bioquest, Inc. (Former ABD Bioquest, Inc.)

従来の蛍光標識試薬の欠点を克服した、安定した強い蛍光性を持つ色素です。

背景

Tide Fluor™色素は、FRETオリゴヌクレオチドやペプチドを開発するためのビルディングブロックとして最適化されています。Tide Fluor™色素は、クマリン、フルオレセイン、ローダミン、シアニンなどの従来の標識色素よりも強い蛍光と高い光安定性を備え、オリゴヌクレオチドやペプチドを標識するための最も手頃な蛍光色素です。

図1. Tide Fluor™色素の発光スペクトル

図2. TF3、他社蛍光色素AおよびBの各蛍光量子収率

図3. TF3、他社蛍光色素AおよびBの各蛍光量子収率

特長

■**Tide Fluor™ 色素発光色/励起レーザー一覧**
色素	Ex (nm)	Em (nm)	ε¹	φ2	CF at 260 nm³	CF at 280 nm⁴	類似スペクトルを持つ色素
Tide Fluor™ 1	345	442	20000	0.95	0.246	0.187	EDANS
Tide Fluor™ 2WS	502	525	75000	0.9	0.211	0.091	Alexa Fluor®488
Tide Fluor™ 2	500	527	75000	0.9	0.288	0.201	FAM, FITC, Alexa Fluor®488
Tide Fluor™ 3WS	555	565	150000	0.105	0.079	0.079	Cy3®, Alexa Fluor®555
Tide Fluor™ 3	555	584	85000	0.85	0.331	0.201	Cy3®, Alexa Fluor®555
Tide Fluor™ 4	590	618	90000	0.91	0.489	0.436	ROX, Texas Red®, Alexa Fluor®594
Tide Fluor™ 5WS	649	664	250000	0.25	0.023	0.027	Cy5®, Alexa Fluor®647
Tide Fluor™ 6WS	676	695	220000	0.18	0.111	0.009	Cy5.5®, IRDye®700, Alexa Fluor®680
Tide Fluor™ 7WS	749	775	275000	0.12	0.009	0.049	Cy7®, Alexa Fluor®750
Tide Fluor™ 8WS	775	807	250000	0.08	0.103	0.109	IRDye®800

ε = Extinction coefficient (cm^-1M^-1) at their maximum absorption wavelength
φ = Fluorescence quantum yield in aqueous buffer (pH 7.2)
CF at 260 nm is the correction factor used for eliminating the dye contribution to the absorbance at 260 nm (for oligos and nucleic acid labeling)
CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptide and protein labeling)

Tide Fluor™ 1

Ex/Em (nm)= 345/442
EC (cm^-1M^-1) = 20,000
CF®280 nm = 0.187
CF®260 nm = 0.246

品名	メーカー	品番	包装	希望販売価格
Tide Fluor^TM 1 azide [TF1 azide]	ABD	2236	5 MG	￥48,000
Tide Fluor^TM 1 alkyne [TF1 alkyne]	ABD	2237	5 MG	￥48,000
Tide Fluor^TM 1 acid [TF1 acid] Superior replacement for EDANS	ABD	2238	100 MG	￥48,000
Tide Fluor^TM 1 amine [TF1 amine] Superior replacement for EDANS	ABD	2239	5 MG	￥48,000
Tide Fluor^TM 1 maleimide [TF1 maleimide] Superior replacement for EDANS	ABD	2242	5 MG	￥48,000
Tide Fluor^TM 1 succinimidyl ester [TF1 SE]Superior replacement for EDANS	ABD	2244	5 MG	￥48,000

Tide Fluor™ 2

Ex/Em (nm)= 500/527
EC (cm^-1M^-1) = 75,000
CF®280 nm = 0.201
CF®260 nm = 0.288

品名	メーカー	品番	包装	希望販売価格
Tide Fluor^TM 2 acid [TF2 acid] Superior replacement for fluorescein	ABD	2245	25 MG	￥48,000
Tide Fluor^TM 2 amine [TF2 amine] Superior replacement for fluorescein	ABD	2246	1 MG	￥48,000
Tide Fluor^TM 2 maleimide [TF2 maleimide] Superior replacement for fluorescein	ABD	2247	1 MG	￥48,000
Tide Fluor^TM 2, succinimidyl ester [TF2 SE]Superior replacement for fluorescein	ABD	2248	5 MG	￥48,000
Tide Fluor^TM 2 azide [TF2 azide]	ABD	2252	1 MG	￥48,000
Tide Fluor^TM 2 alkyne [TF2 alkyne]	ABD	2253	1 MG	￥48,000

Tide Fluor™ 2WS

Ex/Em (nm)= 502/525
EC (cm^-1M^-1) = 75,000
CF®280 nm = 0.091
CF®260 nm = 0.211

品名	メーカー	品番	包装	希望販売価格
Tide Fluor^TM 2WS acid [TF2WS acid] Superior replacement for FITC	ABD	2348	10 MG	￥96,000
Tide Fluor^TM 2WS succinimidyl ester [TF2WS SE] Superior replacement for FITC	ABD	2349	5 MG	￥96,000
Tide Fluor^TM 2WS maleimide [TF2WS Maleimide] Superior replacement for FITC	ABD	2350	1 MG	￥48,000
Tide Fluor^TM 2WS Amine [TF2WS amine] Superior replacement for FITC	ABD	2351	1 MG	￥48,000

Tide Fluor™ 3

Ex/Em (nm)= 555/584
EC (cm^-1M^-1) = 85,000
CF®280 nm = 0.201
CF®260 nm = 0.331

品名	メーカー	品番	包装	希望販売価格
Tide Fluor^TM 3 azide [TF3 azide]	ABD	2254	1 MG	￥48,000
Tide Fluor^TM 3 alkyne [TF3 alkyne]	ABD	2255	1 MG	￥48,000
Tide Fluor^TM 3 acid [TF3 acid] Superior replacement for Cy3	ABD	2268	25 MG	￥48,000
Tide Fluor^TM 3 amine [TF3 amine] Superior replacement for Cy3	ABD	2269	1 MG	￥48,000
Tide Fluor^TM 3 maleimide [TF3 maleimide] Superior replacement for Cy3	ABD	2270	1 MG	￥48,000
Tide Fluor^TM 3 succinimidyl ester [TF3 SE]Superior replacement for Cy3	ABD	2271	5 MG	￥48,000

Tide Fluor™ 3WS

Ex/Em (nm)= 555/565
EC (cm^-1M^-1) = 150,000
CF®280 nm = 0.079
CF®260 nm = 0.079

品名	メーカー	品番	包装	希望販売価格
Tide Fluor^TM 3WS maleimide [TF3WS maleimide] Superior replacement for Cy3	ABD	2344	1 MG	￥48,000
Tide Fluor^TM 3WS acid [TF3WS acid] Superior replacement for Cy3	ABD	2345	10 MG	￥72,000
Tide Fluor^TM 3WS succinimidyl ester [TF3WS SE] Superior replacement for Cy3	ABD	2346	5 MG	￥72,000
Tide Fluor^TM 3WS amine [TF3WS amine] Superior replacement for Cy3	ABD	2347	1 MG	￥48,000

Tide Fluor™ 4

Ex/Em (nm)= 590/618
EC (cm^-1M^-1) = 90,000
CF®280 nm = 0.436
CF®260 nm = 0.489

品名	メーカー	品番	包装	希望販売価格
Tide Fluor^TM 4 acid [TF4 acid] Superior replacement for ROX and Texas Red	ABD	2285	10 MG	￥48,000
Tide Fluor^TM 4 amine [TF4 amine] Superior replacement for ROX and Texas Red	ABD	2286	1 MG	￥48,000
Tide Fluor^TM 4 maleimide [TF4 maleimide] Superior replacement for ROX and Texas Red	ABD	2287	1 MG	￥48,000
Tide Fluor^TM 4, succinimidyl ester [TF4 SE]Superior replacement for ROX and Texas Red	ABD	2289	5 MG	￥48,000
Tide Fluor^TM 4 azide [TF4 azide]	ABD	2300	1 MG	￥48,000
Tide Fluor^TM 4 alkyne [TF4 alkyne]	ABD	2301	1 MG	￥48,000

Tide Fluor™ 5WS

Ex/Em (nm)= 649/664
EC (cm^-1M^-1) = 250,000
CF®280 nm = 0.027
CF®260 nm = 0.023

品名	メーカー	品番	包装	希望販売価格
Tide Fluor^TM 5WS azide [TF5WS azide]	ABD	2275	1 MG	￥48,000
Tide Fluor^TM 5WS alkyne [TF5WS alkyne]	ABD	2276	1 MG	￥48,000
Tide Fluor^TM 5WS acid [TF5WS acid] Superior replacement for Cy5	ABD	2278	10 MG	￥72,000
Tide Fluor^TM 5WS amine [TF5WS amine] Superior replacement for Cy5	ABD	2279	1 MG	￥48,000
Tide Fluor^TM 5WS maleimide [TF5WS maleimide] Superior replacement for Cy5	ABD	2280	1 MG	￥48,000
Tide Fluor^TM 5WS succinimidyl ester [TF5WS SE]Superior replacement for Cy5	ABD	2281	5 MG	￥72,000

Tide Fluor™ 6WS

Ex/Em (nm)= 676/695
EC (cm^-1M^-1) = 220,000
CF®280 nm = 0.009
CF®260 nm = 0.111

品名	メーカー	品番	包装	希望販売価格
Tide Fluor^TM 6WS acid [TF6WS acid] Superior replacement for Cy5.5	ABD	2291	10 MG	￥48,000
Tide Fluor^TM 6WS amine [TF6WS amine] Superior replacement for Cy5.5	ABD	2292	1 MG	￥48,000
Tide Fluor^TM 6WS maleimide [TF6WS maleimide] Superior replacement for Cy5.5	ABD	2293	1 MG	￥48,000
Tide Fluor^TM 6WS succinimidyl ester [TF6WS SE]Superior replacement for Cy5.5	ABD	2294	1 MG	￥24,000
Tide Fluor^TM 6WS azide [TF6WS azide]	ABD	2302	1 MG	￥48,000
Tide Fluor^TM 6WS alkyne [TF6WS alkyne]	ABD	2303	1 MG	￥48,000

Tide Fluor™ 7WS

Ex/Em (nm)= 749/775
EC (cm^-1M^-1) = 275,000
CF®280 nm = 0.049
CF®260 nm = 0.009

品名	メーカー	品番	包装	希望販売価格
Tide Fluor^TM 7WS azide [TF7WS azide]	ABD	2304	1 MG	￥48,000
Tide Fluor^TM 7WS alkyne [TF7WS alkyne]	ABD	2305	1 MG	￥48,000
Tide Fluor^TM 7WS acid [TF7WS acid] Superior replacement for Cy7	ABD	2330	10 MG	￥48,000
Tide Fluor^TM 7WS amine [TF7WS amine] Superior replacement for Cy7	ABD	2331	1 MG	￥48,000
Tide Fluor^TM 7WS maleimide [TF7WS maleimide] Superior replacement for Cy7	ABD	2332	1 MG	￥48,000
Tide Fluor^TM 7WS, succinimidyl ester [TF7WS SE]Superior replacement for Cy7	ABD	2333	1 MG	￥24,000

Tide Fluor™ 8WS

Ex/Em (nm)= 775/807
EC (cm^-1M^-1) = 250,000
CF®280 nm = 0.109
CF®260 nm = 0.103

品名	メーカー	品番	包装	希望販売価格
Tide Fluor^TM 8WS azide [TF8WS azide] Near Infrared Emission	ABD	2306	1 MG	￥48,000
Tide Fluor^TM 8WS alkyne [TF8WS alkyne] Near Infrared Emission	ABD	2307	1 MG	￥48,000
Tide Fluor^TM 8WS acid [TF8WS acid] Near Infrared Emission	ABD	2335	10 MG	￥48,000
Tide Fluor^TM 8WS amine [TF8WS amine] Near Infrared Emission	ABD	2336	1 MG	￥48,000
Tide Fluor^TM 8WS maleimide [TF8WS maleimide] Near Infrared Emission	ABD	2337	1 MG	￥48,000
Tide Fluor^TM 8WS, succinimidyl ester [TF8WS SE]Near Infrared Emission	ABD	2338	1 MG	￥24,000

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※ 表示価格について

「オリゴヌクレオチド標識試薬 Tide Fluor™ Dye」は、下記のカテゴリーに属しています。

オリゴヌクレオチド、ペプチドへの標識また各FRETのドナー構築に最適な蛍光試薬オリゴヌクレオチド標識試薬 Tide Fluor™ Dye

背景