Anti eEF1a elongation factor - Cosmo Bio Co.,Ltd.



Anti eEF1a elongation factor Antibody

Catalog No.: AGR-AS10 934
Size: 200UL
Price: ¥115000
antigen/source: eEF1a
host: Rabbit
Purity: Serum
Application: Western Blot
Storage: -20C
Immunogen: Arabidopsis thaliana
Reacts with: Arabidopsis thaliana
Shape: Lyophilized
Molecular Weight: 49.5 kDa
Purpose: Western blot: 1 : 1000
Optimal dilutions/concentrations should be determined by the end user.


5 g of total protein from Arabidopsis thaliana (A), Hordeum vulgare (B), Zea mays (C), Physcomitrella patens (P) extracted with Agrisera PEB buffer were separated on 4-12 % SDS-PAGE and blotted 70 min to PVDF. Blots were blocked with ECL Advance Blocking Reagent (GE Healthcare) for 1h at room temperature (RT) with agitation. Blot was incubated for 1 h with either rabbit pre-serum (1: 10 000), rabbit anti-eEF1alpha antibody (1: 10 000) and anti-eEF1 alpha serum incubated with eEF1alpha synthetic peptide (in 100 mM excess to saturate a signal from specific antibody) for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera ) diluted to 1:50 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 2 minutes.