||4C & -20C
||5 pg / mL
||The Microwell Estradiol ELISA is an enzyme immunoassay system for quantitative determination of Estradiol levels in rodent serum. The test is intended for professional use as research tool in monitoring of conditions related to serum/plasma levels of estradiol. The test kit is designed to be used by a trained, skilled laboratory professional only.
1. Microtiter wells 96, coated with second antibody.
2. Rabbit anti E2 antibody 7.0 mL
3. Enzyme Conjugate solution, 12 mL.
4. TMB Color Reagent, 12 mL
5. Stopping Solution (2N HCL), 6 mL
6. 20 X Wash Buffer, 20 mL.
7. Sample diluent, 20 mL
8. E2 Standard set: 0,10, 30,100, 300, 1200, 5000pg/mL. (+ QC1 & QC2)
The E2 quantitative test is based on a solid-phase enzyme immunoassay based on competitive binding method. A sample (serum/ plasma/urine) containing an unknown amount of E2 to beassayed (unlabeled antigen) is added to a standard amount of a conjugated E2 (labeled antigen). The labeled and unlabeled antigens are then allowed to compete for high affinity binding sites of E2 antibody on a limited number of secondary antibodiescoated on to the plate. After washing away the free antigen, the amount of labeled antigen in the sample is reversibly proportional to the concentration of the unlabeled antigen. The actual concentrations in unknown samples are obtained by means ofastandard curve based on known concentrations of unlabeled antigen analyzed in parallel with the unknowns. After washing, substrate solution is added and the enzyme is allowed to react for a fixed time before the reaction is terminated. Absorbenciesare measured at 450 nm using ELISA plate reader. A standard curve is produced using values from 5 standards from which absorbency values for blank tubes have been subtracted. Results for unknown may be read directly from this standard curve using either manual calculation or by a suitable computer program. This kit is suitable for the direct measurement of E2 in serum samples