LexA repressor - Cosmo Bio Co.,Ltd.



LexA repressor

Catalog No.: BAM-01-006-EX
Size: 100UG
Price: ¥126000
Description: Recombinant, E.coli
catalog info: Catalog 2012-p161
Storage: -70C
Immunogen: Escherichia coli
Purity: >90% by SDS-PAGE
component: [Specification]
Purity: Over 90% by SDS-PAGE (CBB staining) Protein concentration: 0.8 mg / ml as measured by BCA method
50% glycerol, 10 mM Tris-HCl (pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol


E. coli LexA protein binds specifically to the SOS-box sequence and represses the genes belonging to the SOS regulon. In response to DNA damage, RecA protein is activated by ss-DNA accumulated in the damaged cells and promotes autocleavage of LexA repressor by its coprotease activity. As the results, DNA repair genes and error prone polymerases are induced, and DNA damage is repaired and mutation is induced (1). The lexA gene is used for yeast two-hybrid experiments as a bate toidentify the protein-protein interaction in vivo (2).
1) Studies on the mechanism of E. coli SOS response.
2) Used as an antigen for positive control in Western blotting to confirm that the Bait construct is expressed stably in the nucleusas protein of the expected size in the yeast two-hybrid method using the lexA gene. See also antibody to LexA protein (#61-001, #61-002).


Fig.1 Polyacrylamide gel electrophoresis of LexA protein


•  Waker GC, Cold Spring Harb. Symp. Quant. Biol. 65:1-10 (2000)
•  Sambrook J & Russell DW, Cold Spring Harber Laboratory Press Chapter 1>17-1>27 (2001)