Taq DNA polymerase (-dNTPs), with Standard buffer - Cosmo Bio Co.,Ltd.



Taq DNA polymerase (-dNTPs), with Standard buffer

Catalog No.: BAM-02-011-EX
Size: 200UNIT
Price: ¥3000
catalog info: Catalog 2012-p210
Storage: -20C
Purity: >98% by SDS-PAGE
Purpose: For PCR reactions
component: [Concentration]
5 units/ ul , where one unit is defined as the amount of enzyme that can incorporate 10 nmols of total dNTPs into an acid-insoluble material in 30 minutes at 74C when activated salmon sperm DNA was used as template/primer.
20mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% glycerol, 0.5% Tween20, 0.5% Igepal CA-630


Thermus aquaticus DNA polymerase (Taq DNA polymerase) gene was expressed in E. Coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and the MW is 94 kDa, same as that of the natural enzyme.
This enzyme is suitable for PCR reactions; capable of amplifying DNA with various primers.
High-throughput PCR
Colony PCR
Incorporation of dUTP, dITP, and fluorescence-labeled nucleotides
Primer extension
Addition of asingle nucleotide (adenosine) at the 3'-blunt ends


Fig.1SDS-PAGE of Taq DNA polymerase


Fig.2 Amplification of λDNA