HIV-1 Gag p15 - Cosmo Bio Co.,Ltd.



HIV-1 Gag p15

Catalog No.: BAM-05-007-EX
Size: 20UG
Price: ¥29000
Application: Western Blot
Enzyme Linked Immunosorbent Assay
Dot Blot
catalog info: Catalog 2012-p166
Storage: -20C
Immunogen: Human Immunodeficiency Virus
Purity: >98% by SDS-PAGE
Purpose: [Application]
> It can be used as a substrate for HIV-1 protease in the presence of HIV-1 genomic RNA.
> It can be used in studies of structure and function of AIDS virus as precursor of nucleocapsid p7 protein that binds to HIV-1 genome RNA.
> It can be used as p15 antigen in detection of anti-HIV-1 p15 antibody in Western blotting or ELISA.
> It can be used as standard for the quantitative analysis of HIV-1 p15 antigen.
component: [Purity]
Over 90% by SDS-PAGE (CBB staining)
[Protein concentration]
0.42 mg / ml as measured by BCA method
50% glycerol, 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol


HIV-1 Gag p15 is processed by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is further digested into nuclocapsid protein p7 and into p6 and p1 of unknown function. This digestion is promoted by the bindingof HIV-1 genome RNA and the two Zn finger motifs that exist in the p7 region. The produced nucleocapsid protein p7 regulates the RNA function by directly binding to HIV-1 genome RNA (1). The product is over-expressed as a recombinant protein in E.coli with a plasmid carrying the Gag p15 coding region of HIV-1 virus, subtype B (2), and highly purified by several steps of chromatography (3). Its molecular size is 15 kD, same as that of p15 purified from AIDS virus particles (Fig 1).


Fig. SDS-PAGE of HIV-1 p15


•  Freed EO, Virology 251:1-15 (1998)
•  Adachi A. et al., J. Virol. 59, 284 (1986)
•  Saito A. et al., Microbiol. Immunol. 39:473-483 (1995)