Rad52 Protein - Cosmo Bio Co.,Ltd.



Rad52 Protein

Catalog No.: BAM-10-003-EX
Size: 20UG
Price: ¥42000
Description: Recombinant, E.coli
catalog info: Catalog 2012-p162
Storage: -70C
Immunogen: Human
Purity: >98% by SDS-PAGE
Purpose: [Applications]
> Studies on homologous recombination in mammals including human.
> Studies on the interaction of Rad52 protein with various proteins.
> To be used as a standard for western blotting.
component: [Form]
>0 mg / ml in 20 mM potassium phosphate pH 7.5 200 mM KCl, 2 mM 2ME, 0.5 mM EDTA, 20% glycerol
> 90% as judged from SDS-PAGE analysis (Fig.1)


Human Rad52 protein plays a major role in genetic recombination and recombination repair by mediating strand-annealing reaction between complementary DNA strands (1). Rad52 functionally and physically interacts with Rad51 Dmc1 and RPA in recombination processes. In radiation damaged cell, Rad52 is phosphorylated at Tyr104 by c-Abl tyrosine kinase and Forms nuclear foci (2). The product was highly purified from E. coli over-expressing human Rad52 protein as a recombinant protein (3


Fig.1 Analysis of purified human Rad52 protein by SDS-PAGE


•  Friedberg EC, et al., DNA Repair and Mutagenesis 2, ASM Press
•  Kitao H & Yuan Z M, J. Biol. Chem. 277:48944 (2002)
•  Kurumizaka H et al., J Biol Chem 276: 35201 (2001)