Anti Dnmt1 (1-248) - Cosmo Bio Co.,Ltd.



Anti Dnmt1 (1-248) Antibody

Catalog No.: BAM-70-201-EX
Size: 50UG
Price: ¥26300
antigen/source: Dnmt1 (1-248)
host: Rabbit
Description: Recombinant
Label: Unlabeled
Purity: Affinity Purified
Application: Western Blot
Immuno Fluorescence
catalog info: Catalog 2012-p35
Storage: -20C
Immunogen: Mouse
Reacts with: Mouse
Purity: Affinity Purified
Shape: Liquid


Figure Use of anti-Dnmt1 (1-248) antibody for Western blotting and immuno-fluorescence staining a, Subnuclear localization of Dnmt1 (green), Np95 (red) and Pcna (blue) in mouse embryonic stem cells (E14) during cell-cycle progression (bottom). Merged images for Np95 and Dnmt1 are shown. Profile of DNA content in exponentially growing ECSs is shown (top). b, Association of Np95 with Dnmt1 and Pcna in HeLa cell nuclear extracts. Human NP95 was tagged by biotin-binding domain and stably expressed in HeLa cells together with the E. coli BirA biotin ligase gene. Biotylated Np95 was captured by streptoavidin beads. The captured proteins were eluted from the beads and analyzed by Western bloting using antibodies against Dnmt1 and Pcna. c, Catalytic activity of NP95 associated Dnmt1 in HeLa cells. The presence of Dnmt1 in the NP95 complexes was confirmed by Western blot and the DNA methyltransferase activity of the extracts was measured. (The data were kindly provided by Dr. M. Muto of Riken Research Center for Allergy and Immunology.)