Anti Dnmt3b - Cosmo Bio Co.,Ltd.



Anti Dnmt3b Antibody

Catalog No.: BAM-70-206-EX
Size: 100UG
Price: ¥35000
antigen/source: Dnmt3b
host: Rabbit
Label: Unlabeled
Purity: Affinity Purified
Application: Western Blot
Chromatin Immunoprecipitation
Immuno Fluorescence
catalog info: Catalog 2012-p35
Storage: -20C
Immunogen: Mouse
Reacts with: Human
Accession No: A068626
Purity: Affinity Purified
Shape: Liquid


Fig.1 Western blotting of Dnmt3b. The amounts of Dnmt3b in mouse embryos at the stages of E7.0-E16.5 were examined by Western blotting. The embryos were solubilized by sonication or homogenization in the presence of 0.1% SDS. The dissected embryo (10 ug protein) at each stage was subjected to Western blotting with this antibody. Dnmt3b was highly expressed in E7.0 embryo but decreased thereafter and was below the detection level after E10.5. cDNAs of Dnmt3b isoforms (3b1, 3b2, and 3b3) were transiently expressed in 293T cells and these isoforms were also detectable with this antibody.


Fig.2 Expression of Dnmt3b in mouse embyos was examined by immuno-fluorescence staining. Mouse embryos at E4.5, 5.5 and 7.0 were fixed in cold acetone and stained with this antibody or DAPI (blue). The antibody was detected with anti-rabbit IgG conjugated with ALEXA568 (red). The ICM (the inner cell mass) and trophectoderm (T) are indicated by arrows and arrowheads, respectively. The epiblast (E), and the embryonic ectoderm (EE) and extraembryonic region (EX) are indicated by square brackets. Dnmt3b existed at E4.5-7.0, in ICM at E4.5, the epiblast at E5.5, and the embryonic ectoderm at E7.0.