Anti Nup153 - Cosmo Bio Co.,Ltd.



Anti Nup153 Antibody

Catalog No.: BAM-70-315-EX
Size: 200UG
Price: ¥34500
antigen/source: Nup153
host: Rat
Purity: IgG fraction
Application: Western Blot
Enzyme Linked Immunosorbent Assay
catalog info: Catalog 2012-p71
Storage: -20C
Immunogen: Rat
Isotype: IgG2a
Clone: R4C8
Reacts with: Human
Shape: Steri. and Liq.


Fig.1@Detection of Nup153 by Western blotting with antibody R4C8. Sample is the nuclear membrane fraction of HeLa cells (Left) and NIH3T3 cells (Right)


Fig.2@Immunofluorescent staining of HeLa cells with antibody R4C8.


Fig.3@Immunofluorescent staining of rat neuron with antibody R4C8. The dots are Nup153.


Fig 4.Immunofluorescenct staining of MEF cells from E14.5 mouse embryo with antibody R4C8. Cells fixed with 10% formalin at room temperature for 10 min and permeabilized with ice-cold methanol on ice for 10 min. Cells were blocked with 3% BSA/PBS at room temperature for 30 min and incubated with Nup153 (1:200) antibodies at 4°C overnight, and treated with Alexa-488-cojugated rat IgG (1:1000) at room temperature for 1hr. Chromosomal DNA was detected by staining with 3.3 µM Hoechst 33342. Nuclear peripheries were stained with anti-Nup153 antibody R4C8.