Anti KDM5A/RBP2/JARID1A - Cosmo Bio Co.,Ltd.



Anti KDM5A/RBP2/JARID1A Antibody

Catalog No.: BAM-71-177-EX
Size: 50UG
Price: see also
Size 100
antigen/source: RBP2/JARID1A
host: Mouse
Label: Unlabeled
Purity: IgG fraction
Application: Western Blot
Flow Cytometry
catalog info: Catalog 2012-p83
Storage: -20C
Immunogen: Human
Isotype: IgG2a
Clone: 18E8
Reacts with: Human
Shape: Steri. and Liq.


Fig.1@ Identification of RBP2 in crude cell extracts by Western blotting with antibody 18E8. Samples: 1. HeLa control siRNA @ 2. HeLa RBP2 siRNA @ 3. MCF7@ 4. U2OS @5. NIH3T3 6. J1 (mouse ES)


Fig.2 Immunofluorescence staining of HeLa cell with anti-RBPh antibody. 1. HeLa cells were fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min. 2. Incubate cells with 1.5% BSA in PBS for 30 min to block non-specific binding of the antibodies. Incubate the cells with 1/2,000 diluted anti-RBP2 antibody (18E8) in 1% BSA in PBS at 4°C overnight. 3. Incubate cells with a secondary antibody, goat anti-mouse IgG conjugated with Alex 488, at 1/1,000 dilution in 1% BSA for 1 hr at room temperature. 4. Nucleus (DNA) was stained with DAPI