Anti Necdin - Cosmo Bio Co.,Ltd.



Anti Necdin Antibody

Neurally differentiated embryonal carcinoma-derived protein

Catalog No.: BAM-74-100-EX
Size: 100UL
Price: ¥34500
antigen/source: Necdin
host: Rabbit
Label: Unlabeled
Purity: Serum
Application: Western Blot
Immunocytochemistry (cell)
catalog info: Catalog 2012-p69
Storage: -20C
Immunogen: Mouse
Clone: NC243
Reacts with: Human
Shape: Liquid


Fig.1 Western blotting using this antibody. Western blot analysis of necdin and MAGE-D1 in mouse embryonal tissues (ref.3). Tissue lysates (20 ug) from E18.5 embryos were separated by SDS-PAGE and immunoblotted with antibodies against necdin, MAGE-D1 (D1) and tubulin. Endogenous ~43-kDa necdin protein was detected almost exclusively in the brain and skeletal muscle whereas endogenous ~85-kDa MAGE-D1 protein was expressed in a ubiquitous manner.


Fig.2 Immunohistochemistry using this antibody (ref.3). Fluorescence immunohistochemistry for necdin and MAGE-D1 in mouse embryos. Adjacent sections of the forebrain at E12.5 embryo (upper panels) and the hind limb at E14.5 (lower panels) were stained with antibodies against necdin and MAGE-D1. The arrows point to the preplate (upper panels) and the skeletal muscles (lower panels). The arrowheads indicate the ventricular proliferative zone; V, ventricle; C, bone cavity. Necdin and MAGE-D1 were concentrated in the preplate of the forebrain at E12.5 and skeletal muscle tissues in the hind limb at E14.5. In developing neural tube, MAGE-D1 immunoreactivity was distributed in the ventricular zone as well as the marginal zone.


Fig.3 Immunocytochemistry using this antibody (ref.4). Primary cerebellar granule neurons (CGNs) were fixed and double stained for necdin and postmitotic neuron marker, NeuN. Chromosomal DNA was stained with Hoechst 33342. Arrowheads point to CGNs, and arrows point to CGN progenitors or non-neuronal cells. Necdin-immunoreactive cells overlapped with NeuN-immunoreactive cells. Necdin immunoreactivity was distributed in both the nucleus and the cytosol.