Anti hnRNP-U / SAF-A - Cosmo Bio Co.,Ltd.



Anti hnRNP-U / SAF-A Antibody

scaffold attachment factor A, SAF-A

Catalog No.: BAM-70-415-EX
Size: 100UL
Price: ¥33000
antigen/source: hnRNP-U / SAF-A
host: Rabbit
Description: [Immunogen]a.a. 614-800
Purity: Serum
Application: Western Blot
Immuno Fluorescence
catalog info: Catalog 2012-p51
Storage: -20C
Immunogen: Mouse
Reacts with: Human
Shape: Liquid


Fig.1 Immunoblotting of hnRNP-U with this antibody (ref.2). Specificity of anti-hnRNP-U antibody, HUT. Cell lysates were prepared from SAOS-2 cells transfected with pRc/CMV vectors (pRc) or pRc/CMV vectors expressing Myc-tagged hnRNP-U (Myc-UF). Exogenous Myc-tagged hnRNP-U (Myc-U) and endogenous hnRNP-U (U) proteins were detected by immunoblotting with anti-Myc antibody (αMyc) or HUT (αU). This antibody recognized exogenous Myc-tagged hnRNP-U and endogenous ~120 kDa hnRNP-U proteins in SAOS-2 cells.


Fig.2 Immunocytochemistry using this antibody, HUT (ref.2) Mouse P19 neurons were labeled with anti-necdin antibody (Ndn) (a) or with HUT for hnRNP-U (U) (c) in combination with anti-neuronal marker, MAP2, antibody for MAP2 (b, d). The nuclear matrix was prepared in situ and labeled for necdin (Ndn) (e), hnRNP-U (U) (f), and a nuclear matrix marker, lamin B (g). Both necdin and hnRNP-U were localized to the nuclei of differentiated neurons, which express the neuronal marker MAP2 (a-d). Necdin was also distributed in the neuronal cytoplasm (a). The immunocytochemical analysis of in situ extracted nuclear matrix revealed that both necdin and hnRNP-U were concentrated in intranuclear speckles throughout the nucleoplasm (e, f). Lamin B, a nuclear matrix marker, was localized to the nuclear lamina (g). These results suggest that both necdin and hnRNP-U are associated with the nuclear matrix of neurons.