Anti Nestin - Cosmo Bio Co.,Ltd.



Anti Nestin Antibody

Catalog No.: BAM-73-105-EX
Size: 100UL
Price: ¥34500
antigen/source: Nestin
host: Rabbit
Description: [Immunogen]C-terminal, 15 a.a.
Purity: Serum
Application: Western Blot
Immunocytochemistry (cell)
catalog info: Catalog 2012-p69
Storage: -20C
Immunogen: Mouse
Clone: ST1
Reacts with: Mouse
Not Reacts with: Human
Shape: Liquid


Fig.1 Detection of Nestin in whole extracts of mouse neocortex and neural stem cells by western blotting with anti-Nestin antibody (ST1). Lane 1; Mouse neucortex at E14.5 Lane 2; Neural stem cells in culture prepared from E14.5 mouse neocortex. Lane 3; Neurons in culture differentiated from neural stem cells. Nestin is detected only in undifferentiated neural stem cells 1) 10% SDS-PAGE was used. 2) Blocking with 5% skim milk in PBST. 3) Anti-Nestin antibody (ST1) was used at 1/500 dilution. 4) As 2nd antibody, HRP-conjugated polyclonal anti-rabbit IgG antibody was used at 1/20,000. 5) As a loading control, western blot of γ-tubulin was employd. (* Stars indicate non-specific bands.)


Fig.2 Immunofluorescence staining of nestin in mouse neuroepithelial cells by using anti-nestin antibody (ST1). Cells in culture were fixed with paraformaldehyde and reacted with anti-nestin antibody at 1/300 dilution and then with rhodamaine-conjugated donky anti-rabbit antibody as a second antibody.


Fig.3. Section of telencephalon of mouse stained by immunohistochemistry with anti-nestin antibody.(ST1) Mouse telencephalon was fixed with paraformaldehyde, and frozen. Sections were cut on a cryostat. The sections were reacted with anti-nestin antibody (ST1) at 1/500 dilution and FITC-conjugated anti-rabbit Ig antibody as a secondary antibody. Scale bar is 10 µm.